Search results for "ELISA"

Assessment of the Optimum Linker Tethering Site of Alternariol Haptens for Antibody Generation and Immunoassay Development

2021

Immunochemical methods for mycotoxin analysis require antigens with well-defined structures and antibodies with outstanding binding properties. Immunoreagents for the mycotoxins alternariol and/or alternariol monomethyl ether have typically been obtained with chemically uncharacterized haptens, and antigen conjugates have most likely been prepared with mixtures of functionalized molecules. For the first time, total synthesis was performed, in the present study, to obtain two haptens with opposite linker attachment locations. The functionalized synthetic haptens were purified and deeply characterized by different spectrometric methods, allowing the preparation of bioconjugates with unequivoc…

La Riforma in Inghilterra: Elisabetta e i 39 Articoli di religione della Chiesa Anglicana

2019

The aim of this article is to underline the doctrinal peculiarities of Anglicanism through examination of the 39 Articles of religion. Unlike other coeval protestant confessions of faith, the genesis of the 39 Articles was eminently dictated by political reasons, that is to say the reinforcement of the English monarchy through the pursuit of religious unity in the Country

A multilight approach for documenting and modeling the appearance of large cultural heritage objects

2022

In recent years, Computer Vision has played a major role in the investigation of sur-face appearance, particularly with the introduction of image-based rendering algorithms. Reflectance transformation Imaging (RTI) is one of these approaches. The RTI is a multi-light imaging technique that involves taking a series of images of an item using a stationary camera, while the location of the incident light varies in each photograph. Thanks to the information gathered from this type of acquisition, which is also defined as a Multi-light Image Collection (MLIC), it is possible to model the angular reflectance of a surface. The RTI is frequently employed in the documentation and study of architectu…

Monoclonal antibody-based immunoassays for cyprodinil residue analysis in QuEChERS-based fruit extracts

2015

Cyprodinil is among the most common agrochemical residues found in highly perishable fruits, such as strawberries. In the present study, high-affinity monoclonal antibodies to this anilinopyrimidine fungicide were raised for the first time with the aim to produce valuable immunochemical analytical assays. Cyprodinil bioconjugates and the generated novel monoclonal antibodies were employed for sensitive competitive immunoassay development in two different formats. The limits of detection of the optimized assays were 20 and 30 ng L-1 for the indirect and direct assay, respectively. Influence over assay parameters of different physicochemical factors was studied. Strawberry samples were extrac…

Desarrollo de métodos inmunoquímicos para el análisis de los fungicidas estrobilurínicos Kresoxim-methyl y Trifloxystrobin

2012

La gran variedad de plaguicidas, su amplio uso y la preocupación que genera la presencia de residuos en muestras agroalimentarias, conlleva la necesidad de disponer de metodologías analíticas fiables que permitan la determinación eficaz de estos residuos en alimentos. En este sentido, los procedimientos analíticos basados en técnicas inmunológicas y obtención de anticuerpos para el análisis de plaguicidas constituyen una alternativa en constante desarrollo. Es precisamente en esta línea en la que se enmarca el objetivo principal de este estudio, consistente en el desarrollo y aplicación de nuevos métodos basados en anticuerpos monoclonales que permitan el análisis rápido y sencillo de los f…

Elisabet Järnefeltin kirjallinen salonki Kauppis-Heikin kirjailijauran käännekohtana

2017

Immunochemical method for penthiopyrad detection through thermodynamic and kinetic characterization of monoclonal antibodies.

2021

Immunoassays are nowadays being employed for rapid contaminant analysis in clinical, environmental, and agrochemical samples. A thorough characterization of the antibody‒antigen interaction can bring light to the immunoreagent selection process in order to develop sensitive and robust tests. Thus, determination of equilibrium and reaction rate constants is usually recommendable. However, this can be quite tricky for low molecular weight compounds, and competitive strategies are commonly followed to estimate apparent affinity values. In the present study, a collection of monoclonal antibodies to penthiopyrad was raised for the first time, and apparent equilibrium constants were assessed by t…

L'impact des formes urbaines dans la mise en place des politiques de transition énergétique : une approche par la modélisation

2019

Rencontres Francophones Transport Mobilité 2019, Montréal, CANADA, 11-/06/2019 - 13/06/2019

Stabilising selection on immune response in male black grouse Lyrurus tetrix

2017

Illnesses caused by a variety of micro- and macro- organisms can negatively affect individuals’ fitness, leading to the expectation that immunity is under positive selection. However, immune responses are costly and individuals must trade-off their immune response with other fitness components (e.g. survival or reproductive success) meaning that individuals with intermediate response may have the greatest overall fitness. Such a process might be particularly acute in species with strong sexual selection because the condition-dependence of male secondary sexual-traits might lead to striking phenotypic differences amongst males of different immune response levels. We tested whether there is s…

Cow's milk–protein allergy as a cause of anal fistula and fissures: A case report

1998

The amplified ELISA assay results correlate well with the standard ELISA results reported previously from our laboratory. Previously it has been necessary to use high flow samplers or long sampling times to measure airborne Fel d 1 concentrations in the range reported here.5 The amplified assay might allow measurements with very short sampling periods to better describe dose-response relationships, and may also be applied to measure airborne concentrations of allergens such as cockroach or dust mite that are airborne at very low concentrations. Our finding that Fel d 1 allergen is measurable in all samples from homes without cats confirms the ubiquitous nature of cat allergen and may help t…