Search results for "Eels"
showing 10 items of 90 documents
Protocol for Specific Isolation of Virulent Strains of Vibrio vulnificus Serovar E (Biotype 2) from Environmental Samples
2004
ABSTRACT The eel pathogen Vibrio vulnificus biotype 2 comprises at least three serovars, with serovar E being the only one involved in both epizootics of eel vibriosis and sporadic cases of human infections. The virulent strains of this serovar (VSE) have only been recovered from clinical (mainly eel tissue) sources. The main objective of this work was to design and validate a new protocol for VSE-specific isolation from environmental samples. The key element of the new protocol is the broth used for the first step (saline eel serum broth [SEB]), which contains eel serum as a nutritive and selective component. This approach takes advantage of the ability of VSE cells to grow in eel serum an…
Field testing of a vaccine against eel diseases caused by Vibrio vulnificus
2001
The field results of a vaccination programme against Vibrio vulnificus serovar E (biotype 2) in a Spanish eel farm are reported. A total of 9.5 million glass eels were vaccinated from January 1998 to March 2000 by prolonged immersion followed by 2 subsequent reimmunisations after 12 to 14 and 24 to 28 d, respectively. The acquired protection and the immune response against serovar E were estimated over a period of 6 mo after vaccination. A similar vaccination schedule was conducted with elvers in a Danish eel farm. In this case, the acquired protection and the immune response against serovar E and the new eel-pathogenic serovars, recently described in Denmark, were evaluated over a short te…
Vibrio vulnificus serovar A: an emerging pathogen in European anguilliculture.
2006
The spread of the emerging pathogen Vibrio vulnificus biotype 2 serovar A in Danish anguilliculture is reported. Serovar A was originally isolated in a Spanish eel farm in 2000 and occurred in Denmark in the summer of 2004, affecting eels of 5-10 g body weight cultured in fresh water. The Danish eels showed clinical signs different from those reported for Spanish eels, such as severe haemorrhages in the head and gill region with necrosis of the soft tissues. Danish isolates were biochemically and serologically identical to Spanish serovar A strains and also highly virulent for eels by both intraperitoneal injection and immersion challenges. Vaccination with Vulnivaccine, a vaccine against V…
Analysis of stamping performances of dual phase steels: A multi-objective approach to reduce springback and thinning failure
2009
The industrial interest on light weight components has contributed in the last years to a significant research effort on new materials able to guarantee high mechanical properties, good formability and reasonable costs together with reduced weights when compared to traditional mild steels. Among such materials advanced high strength steels (AHSS) such as transformations induced plasticity (TRIP) and dual phase (DP), and light weight alloys proved their usefulness in stamping of automotive components. As AHSS are concerned, one of the main drawbacks is related to springback occurrence. Many aspects have to be taken into account when springback reduction is investigated: material behavior iss…
Siderophores and related outer membrane proteins produced by pseudomonads isolated from eels and freshwater.
1992
A total of 46 environmental pseudomonads, together with six type strains, were examined for their siderophore-producing activity. All strains were able to grow under iron-limiting conditions, gave orange halos in the CAS agar assay, and produced hydroxamates, and some of them also produced phenolate-type compounds. Bioassays showed that all strains, except Pseudomonas aeruginosa, promoted growth of mutant strain Arthrobacter flavescens JG-9, deficient in hydroxamate production, and some of them promoted growth of Salmonella typhimurium enb-1, which requires enterobactin for growth. The presence of iron-regulated outer membrane proteins was observed, the molecular size of the main induced pr…
Siderophore-mediated iron acquisition mechanisms in Vibrio vulnificus biotype 2
1996
Vibrio vulnificus biotype 2 is a primary pathogen for eels and, as has recently been suggested, an opportunistic pathogen for humans. In this study we have investigated the ability of V. vulnificus biotype 2 to obtain iron by siderophore-mediated mechanisms and evaluated the importance of free iron in vibriosis. The virulence degree for eels was dependent on iron availability from host fluids, as was revealed by a reduction in the 50% lethal dose for iron-overloaded eels. This biotype produced both phenolate- and hydroxamate-type siderophores of an unknown nature and two new outer membrane proteins of around 84 and 72 kDa in response to iron starvation. No alterations in lipopolysaccharide …
L'intonaco Li Vigni nell'architettura del XX secolo a Palermo. Nuove conoscenze e applicazioni diagnostiche per la manutenzione
2013
Il saggio presenta lo studio degli intonaci formulati per imitare le pietre e i marmi naturali che si diffondono in Sicilia all’inizio del XX secolo ed evidenzia che le prime applicazioni e la diffusione delle nuove malte, anche nel resto dell’Italia, risentono delle sperimentazioni condotte in tutta l’Europa. Il contributo riassume i risultati di una rigorosa e capillare ricognizione tra i brevetti conservati presso l’Archivio Centrale dello Stato a Roma inerenti alle malte per il confezionamento dell’intonaco a “finta pietra”, ne analizza i contenuti in modo critico e pone in risalto i contatti culturali con le sperimentazioni dei materiali in atto nell’ambito dei grandi cantieri di resta…
Effects of salinity and temperature on long-term survival of the eel pathogen Vibrio vulnificus biotype 2 (serovar E)
1999
ABSTRACT Vibrio vulnificus biotype 2 (serovar E) is a primary eel pathogen. In this study, we performed long-term survival experiments to investigate whether the aquatic ecosystem can be a reservoir for this bacterium. We have used microcosms containing water of different salinities (ranging from 0.3 to 3.8%) maintained at three temperatures (12, 25, and 30°C). Temperature and salinity significantly affected long-term survival: (i) the optimal salinity for survival was 1.5%; (ii) lower salinities reduced survival, although they were nonlethal; and (ii) the optimal temperature for survival was dependent on the salinity (25°C for microcosms at 0.3 and 0.5% and 12°C for microcosms at 1.5 to 3.…
L'image documentaire au temps du franquisme
1998
Quelle que soit l'indifférence de NO-DO a l'égard de l'actualité nationale et internationale, il n'en reste pas moins pertinent de déterminer le rôle que les autorités du régime lui conféraient en ce qui concerne la création d'une image (peu importe qu'elle eût été presque intemporelle) de la vie idéale du pays, en accord avec l'idéologie franquiste. 11 n'est pas dû au hasard si NO-DO contribue à bâtir pendant ces années une nouvelle image de Franco et, par là, une nouvelle définition implicite de son régime. Aussi paradoxal que cela puisse paraître, la présence matérielle de Franco en tant que chef d'état augmente â l'intérieur des actualités. Cependant, elle manifeste un virage consistant…
Film Clubs, Festivals, Archives, and Magazines
2012
This chapter deals with the cinema culture as conceived as all the phenomena surrounding cinema but different from the content of the film, namely: festivals, archives and magazines. Its corpus is the Spanish cinema.