Search results for "Embryogenesis"

showing 7 items of 187 documents

Cardiac pacemaker function of HCN4 channels in mice is confined to embryonic development and requires cyclic AMP.

2008

Important targets for cAMP signalling in the heart are hyperpolarization-activated and cyclic nucleotide-gated (HCN) channels that underlie the depolarizing 'pacemaker' current, I(f). We studied the role of I(f) in mice, in which binding of cAMP to HCN4 channels was abolished by a single amino-acid exchange (R669Q). Homozygous HCN4(R669Q/R669Q) mice die during embryonic development. Prior to E12, homozygous and heterozygous embryos display reduced heart rates and show no or attenuated responses to catecholaminergic stimulation. Adult heterozygous mice display normal heart rates at rest and during exercise. However, following beta-adrenergic stimulation, hearts exhibit pauses and sino-atrial…

medicine.medical_specialtymedicine.medical_treatmentCyclic Nucleotide-Gated Cation ChannelsEmbryonic DevelopmentStimulationMice TransgenicBiologyIn Vitro TechniquesGeneral Biochemistry Genetics and Molecular BiologyCardiac pacemakerArticleMiceHeart RatePregnancyInternal medicineHeart ratemedicineCyclic AMPHyperpolarization-Activated Cyclic Nucleotide-Gated ChannelsAnimalsMyocytes CardiacMolecular BiologyIon channelCells CulturedCatecholaminergicGeneral Immunology and MicrobiologyGeneral NeuroscienceEmbryogenesisDepolarizationEmbryoHeartMice Inbred C57BLEndocrinologyMutationFemaleThe EMBO journal
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A time to look back: analysis of morphokinetic characteristics of human embryo development.

2013

Objective To describe the times associated with the morphological changes that occur in the embryo during preimplantation development based on the largest sample size described with time lapse. Design Cohort study. Setting University-affiliated private center. Patient(s) A total of 9,530 embryos from 1,806 intracytoplasmic sperm injection (ICSI) cycles. Intervention(s) None. Main Outcome Measure(s) Using a time-lapse system, embryo images were acquired for at least 68 hours, in some cases reaching 120–130 hours. Embryo cleavage time points up to 8-cell-stage (t2–t8) as well as morulae (tM) and blastocyst formation (tB) were registered in hours after ICSI. Additionally, duration of the cell …

medicine.medical_specialtymedicine.medical_treatmentPopulationEmbryonic DevelopmentBiologyCleavage (embryo)Time-Lapse ImagingIntracytoplasmic sperm injectionBack analysismedicineHumansBlastocystSperm Injections IntracytoplasmiceducationCells CulturedGynecologyeducation.field_of_studyEmbryogenesisCell CycleObstetrics and GynecologyEmbryoEmbryo Mammalianmedicine.anatomical_structureBlastocystReproductive MedicineEmbryologyembryonic structuresFertility and sterility
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Consequences of analgesics use in early pregnancy: Results of tests on mice.

2019

International audience; Self-medication during pregnancy continues to be an issue in developing countries due topoor medical education. The most commonly used drugs are analgesics, mainlyacetaminophen (paracetamol, APAP) and, to a lesser extent, ketoprofen (KPF). The aim of thestudy was to establish whether there are consequences of accidental use of these two drugsduring early embryogenesis. The experimental study was performed on 30 pregnant whitemice, divided into three groups: a first group to which APAP was administered, a secondgroup to which KPF was administered, and group 3 as a control group. At delivery, the babymice were examined, and after their first parturition, they were take…

mice embryogenesisEnvironmental EngineeringNecrosisketoprofen010504 meteorology & atmospheric sciencesparacetamolUrinary systemPhysiology010501 environmental sciencesKidney01 natural sciencesliver.MiceNecrosisPregnancyEctasiamedicineEnvironmental ChemistryAnimalsWaste Management and Disposal0105 earth and related environmental sciencesAcetaminophenPregnancyKidneybusiness.industrydigestive oral and skin physiologyAnalgesics Non-Narcoticmedicine.diseasePollution3. Good healthAcetaminophenmedicine.anatomical_structureLiverHemosiderinToxicity[SDE]Environmental SciencesFemalemicroscopic changesmedicine.symptomChemical and Drug Induced Liver Injurybusinessmedicine.drugThe Science of the total environment
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First stages of microspore reprogramming to embryogenesis through anther culture in Prunus armeniaca L

2009

microspore embryogenesis apricot cell structure cytochemistry
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Molecular Network Establishing Dorsal-Ventral polarity during sea urchin embryogenesis

2015

molecular network dorsal ventral polarity sea urchin embryogenesisSettore BIO/11 - Biologia Molecolare
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Somatic Embryogenesis and Plant Regeneration from Hypocotyl Cultures of Digitalis obscura L.

1986

Summary Hypocotyl sections from 20-day-old seedlings of Digitalis obscura L. were used to examine the effect of IAA on somatic embryogenesis. Although embryogenesis was obtained with all IAA concentrations (0.1, 0.5 and 1 ppm) and under both illumination conditions tested (16 h photoperiod and darkness), incubation in photoperiod increased yield of embryos and shortened the embryo induction time. Optimum results were obtained with 1 ppm IAA. About 30 % of the embryos obtained developed into complete plantlets when transferred to basal medium without growth regulators.

photoperiodismbiologySomatic embryogenesisPhysiologyEmbryogenesisDigitalis obscurafood and beveragesEmbryoPlant Sciencebiology.organism_classificationHypocotylTissue cultureBotanyAgronomy and Crop ScienceIncubationJournal of Plant Physiology
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Morphological and Anatomical Observations of Abnormal Somatic Embryos from Anther Cultures of Citrus reticulata Blanco.

2010

somatic embryogenesis mandarinanatomy in vitro cultureCitrus in vitro culture
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