Search results for "Erich"

'Viva Alemania': internados alemanes de Camerún en España durante la Primera Guerra Mundial

2019

espanolUn episodio historico poco conocido en el ambito de las relaciones interculturales germano-espanolas y de los procesos migratorios, pero cuyas huellas son todavia palpables en la actualidad, es el de la internacion de la llamada Schutztruppe (fuerza militar colonial alemana) de Camerun en Espana durante la Primera Guerra Mundial. Lo sorprendente en esta situacion de crisis politica se reflejo en el entusiasmo y la admiracion con que fueron recibidos en un primer momento los llamados “internados” alemanes en un pais supuestamente neutral y que se encontraba oficiosamente dividido entre aliadofilos y germanofilos. El ensayo es el resultado de una minuciosa busqueda de las huellas liter…

UV-induced cross-linking of proteins to plasmid pBR322 containing 8-azidoadenine 2′-deoxyribonucleotides

1988

Abstract An efficient method of cross-linking DNA to protein is described. The method is based on the incorporation of photoactive 8-azidoadenine 2′-deoxyribonucleotides into DNA. We have found that 8-N 3 dATP is a substrate for E. coli DNA polymerase I and that 8-N 3 dATP can be incorporated into plasmid pBR322 by nick-translation. Subsequently we were able to cross-link a set of different proteins to 8-azido-2′-deoxyadenosine-containing pBR322 by UV irradiation (366 nm). No DNA-protein photocross-linking was observed under the same conditions when the non-photoactive plasmid pBR322 was used.

Cloning and characterization of CSP37, a novel gene encoding a putative membrane protein of Candida albicans.

1997

In the course of an analysis of the functions and assembly of the cell wall of Candida albicans, we have cloned and characterized a gene, which we designated CSP37 (cell surface protein), encoding a 37-kDa polypeptide which is a membrane-associated protein. The gene was isolated by immunological screening of a DNA library constructed from mycelial cells with a polyclonal serum raised against cell walls of this morphology. Analysis of the nucleotide sequence of a corresponding genomic DNA fragment revealed a single open reading frame which encodes a predicted protein of 321 amino acids with no significant homology to others in the databases. Disruption of the CSP37 gene by the method describ…

Insertion of a malE B-Galactosidase fusion protein into the envelope of Escherichia coli disrupts biogenesis of outer membrane proteins and processin…

1982

The synthesis of a membrane-bound MalE ,B-galactosidase hybrid protein, when induced by growth of Escherichia coli on maltose, leads to inhibition of cell division and eventually a reduced rate of mass increase. In addition, the relative rate of synthesis of outer membrane proteins, but not that of inner membrane proteins, was reduced by about 50%o. Kinetic experiments demonstrated that this reduction coincided with the period of maximum synthesis of the hybrid protein (and another maltose-inducible protein, LamB). The accumulation of this abnormal protein in the envelope therefore appeared specifically to inhibit the synthesis, the assembly of outer membrane proteins, or both, indicating t…

Prevalence and Antimicrobial Resistance of Escherichia coli, Enterococcus spp. and the Major Foodborne Pathogens in Calves in Latvia

2018

The aim of the present study was to detect the prevalence and antimicrobial resistance of fecal indicators and major foodborne pathogens in feces of calves and to identify the factors associated with increased prevalence of resistant bacteria on farms. Altogether, 180 rectal swabs were collected from 18 farms in Latvia. Samples were investigated to detect the prevalence and antimicrobial resistance of Escherichia coli, Enterococcus spp., Listeria monocytogenes, Yersinia enterocolitica, Salmonella spp., Staphylococcus aureus, and Campylobacter spp. Among all, 64% (74/110) of commensal E. coli, 100% (78/78) Enterococcus faecalis and 96% (22/23) Enterococcus faecium isolates were resistant at …

Extended-Spectrum ß-Lactamase, AmpC-Producing, and Fluoroquinolone-Resistant Escherichia coli in Retail Broiler Chicken Meat, Italy.

2015

Background: Globally, antimicrobial drug-resistant Escherichia coli is among the most common etiological agents of invasive disease in humans. In Europe, increasing proportions of infections due to third-generation cephalosporins and/or fluoroquinolone-resistant extraintestinal pathogenic E. coli (ExPEC) strains are reported. E. coli from poultry are those more closely linked to human E. coli, but lack of reliable data makes it difficult to assess the attributable risk of different food sources. In the present study, our objective was to investigate the antimicrobial resistance profile, phylogenetic background, and virulence factors of E. coli isolates from broiler chicken meat sold at reta…

Relative Frequencies and Significance of Faecal Coliforms as Indicators Related to Water Temperature

1983

Summary The faecal coliforms at different sites of a hypereutrophic lake near Valencia (Albufera) were identified and their relative amounts established along an annual cycle. Using lauryl tryptose broth at 35°C, followed by incubation at 44.4°C in 2% brilliant green bile, Escherichia coli and Klebsiella pneumoniae are practically the only coliforms present. A positive correlation was found between the water temperature and the relative amount of these two coliforms: K. pneumoniae predominates at high water temperatures, whereas E. coli shows preponderance during the cold period. The role of K. pneumoniae as the only faecal indicator under the circumstances described in the work is emphasiz…

GENETICS AND BIOFILM ANALYSIS ON STRAINS OF E.COLI ISOLATED FROM ANIMALS WITH DIARRHOEIC PROBLEMS

2015

Visiting the King of the World

2009

Apsilankymas pas Pasaulio karalių – tai tema, kuri bent paviršutiniškai supažindina su egzistuojančiais daugiau ar mažiau ezoteriniais pasakojimais, kartais net gana fantastiškais, kuriuose kalbama apie viršžmogiškosiomis galiomis apdovanotas būtybes, gyvenančias, atrodo, žemės gelmėse, Azijos žemių platybėse, Himalajų regione, Mongolijoje ar Tibete. „Pasaulio karalius“ – pavadinimas 1927 m. parašyto veikalo, kurio autorius René Guénonas – prancūzų ezoterikas ir mistikas, gimęs 1886 m. Blois, miręs 1951 m. Kaire. Iš literatūros kūrinių – ir kelionių aprašymų, ir fantastinių romanų – be Jules‘io „Kelionės į žemės centrą“ galima paminėti Ferdinando Ossendowskio knygą „Gyvuliai, žmonės ir diev…

Signal sequences modulate the immunogenic performance of human hepatitis C virus E2 gene

2005

Abstract Envelope protein E2 of human hepatitis C virus (HCV) is an attractive component of a prototype HCV vaccine. Delivered by DNA immunogens, E2 evokes specific immune response of Th1-type, failing to induce either considerable antibody production, or T-helper cell proliferation. We aimed at modulating the immunogenic performance of E2 gene by changing the mode of protein expression in eukaryotic cells. Plasmids were constructed encoding full-length E2 and nonstructural protein 1 (p7) fused to either 13 or 38 C-terminal amino acids (aa) of HCV E1 that contain second hydrophobic segment of E1 stop-transfer signal, or a complete E1 stop-transfer signal with duplicated second hydrophobic s…