Search results for "Excitation"

Optimizing MRI contrast with B1 pulses using optimal control theory

2016

The variety of achievable contrasts by MRI makes it a highly flexible and valuable diagnostic tool. Contrast results from relaxation time differences, which are intrinsic properties of each tissue. Using optimal control theory, one can control the obtained contrast by applying excitation pulses that bring the magnetization in a user-defined target state. Simulation results are presented to illustrate the feasibility and the flexibility of using optimal contrast pulses. The robustness to experimental variable parameters such as field inhomogeneities is also studied. Finally, an in-vitro contrast experiment is performed on a small-animal MRI showing a reasonable match with the simulation resu…

Energy harvesting using piezoelectric transducers for suspension systems

2020

Abstract Energy harvesting by using functional materials in suspension systems bear potential to win-back certain (even if low) amounts of vibrational energy, otherwise dissipated via the conventional (passive) dampers. Piezoelectric (PE) ceramics are functional materials that can be used for transforming mechanical energy into electrical and vice versa. In this paper, we study the capabilities and efficiency of energy harvesting (EH) with PE transducers under two different kinds of external excitation: i) Periodic and ii) stochastic. An appropriate nonlinear lumped parameter electromechanical model (LPEM) is brought into the two-port network notation. Laboratory experiments were conducted …

Evaluation of Coded Excitations for Autonomous Airborne Ultrasonic Inspection

2019

Unmanned Aerial Vehicles (UAVs) are receiving increasing attention for use in Non-Destructive Testing due to their ability to access areas where manual inspection is not practical. Contact-based UAV ultrasonic inspections grant the opportunity to remotely monitor the structural health of an industrial asset with enhanced internal integrity information. Ultrasonic inspection is a Non-Destructive Testing (NDT) method conventionally used in corrosion mapping. Surface contacting ultrasonic transducers provide enhanced structural integrity information. However, due to near-surface aerodynamic effects, angular sensitivity of the ultrasound probe and alignment error during autonomous inspections, …

Photoexcitation of the P4480 State Induces a Secondary Photocycle That Potentially Desensitizes Channelrhodopsin-2

2018

Channelrhodopsins (ChRs) are light-gated cation channels. In spite of their wide use to activate neurons with light, the photocurrents of ChRs rapidly decay in intensity under both continuous illum...

2020

Phytochrome proteins control the growth, reproduction, and photosynthesis of plants, fungi, and bacteria. Light is detected by a bilin cofactor, but it remains elusive how this leads to activation of the protein through structural changes. We present serial femtosecond X-ray crystallographic data of the chromophore-binding domains of a bacterial phytochrome at delay times of 1 ps and 10 ps after photoexcitation. The data reveal a twist of the D-ring, which leads to partial detachment of the chromophore from the protein. Unexpectedly, the conserved so-called pyrrole water is photodissociated from the chromophore, concomitant with movement of the A-ring and a key signaling aspartate. The chan…

Fast Regulation of GABAAR Diffusion Dynamics by Nogo-A Signaling.

2019

Summary: Precisely controlling the excitatory and inhibitory balance is crucial for the stability and information-processing ability of neuronal networks. However, the molecular mechanisms maintaining this balance during ongoing sensory experiences are largely unclear. We show that Nogo-A signaling reciprocally regulates excitatory and inhibitory transmission. Loss of function for Nogo-A signaling through S1PR2 rapidly increases GABAAR diffusion, thereby decreasing their number at synaptic sites and the amplitude of GABAergic mIPSCs at CA3 hippocampal neurons. This increase in GABAAR diffusion rate is correlated with an increase in Ca2+ influx and requires the calcineurin-mediated dephospho…

Activation of Corticothalamic Layer 6 Cells Decreases Angular Tuning in Mouse Barrel Cortex.

2019

In the mouse whisker system, the contribution of L6 corticothalamic cells (L6 CT) to cortical and thalamic processing of the whisker deflection direction was investigated. A genetically defined population of L6 CT cells project to infragranular GABAergic interneurons that hyperpolarize neurons in somatosensory barrel cortex (BC). Optogenetic activation of these neurons switched BC to an adapted mode in which excitatory cells lost their angular tuning. In contrast, however, this was not the case with a general activation of inhibitory interneurons via optogenetic activation of Gad2-expressing cells. The decrease in angular tuning, when L6 CT cells were activated, was due to changes in cortic…

4D (x-y-z-t) imaging of thick biological samples by means of Two-Photon inverted Selective Plane Illumination Microscopy (2PE-iSPIM)

2015

AbstractIn the last decade light sheet fluorescence microscopy techniques, such as selective plane illumination microscopy (SPIM), has become a well established method for developmental biology. However, conventional SPIM architectures hardly permit imaging of certain tissues since the common sample mounting procedure, based on gel embedding, could interfere with the sample morphology. In this work we propose an inverted selective plane microscopy system (iSPIM), based on non-linear excitation, suitable for 3D tissue imaging. First, the iSPIM architecture provides flexibility on the sample mounting, getting rid of the gel-based mounting typical of conventional SPIM, permitting 3D imaging of…

Auxiliary α2δ1 and α2δ3 Subunits of Calcium Channels Drive Excitatory and Inhibitory Neuronal Network Development

2020

VGCCs are multisubunit complexes that play a crucial role in neuronal signaling. Auxiliary α2δ subunits of VGCCs modulate trafficking and biophysical properties of the pore-forming α1 subunit and trigger excitatory synaptogenesis. Alterations in the expression level of α2δ subunits were implicated in several syndromes and diseases, including chronic neuropathic pain, autism, and epilepsy. However, the contribution of distinct α2δ subunits to excitatory/inhibitory imbalance and aberrant network connectivity characteristic for these pathologic conditions remains unclear. Here, we show that α2δ1 overexpression enhances spontaneous neuronal network activity in developing and mature cultures of …

Precision and accuracy of single-molecule FRET measurements-a multi-laboratory benchmark study

2018

Single-molecule Forster resonance energy transfer (smFRET) is increasingly being used to determine distances, structures, and dynamics of biomolecules in vitro and in vivo. However, generalized protocols and FRET standards to ensure the reproducibility and accuracy of measurements of FRET efficiencies are currently lacking. Here we report the results of a comparative blind study in which 20 labs determined the FRET efficiencies (E) of several dye-labeled DNA duplexes. Using a unified, straightforward method, we obtained FRET efficiencies with s.d. between +/- 0.02 and +/- 0.05. We suggest experimental and computational procedures for converting FRET efficiencies into accurate distances, and…