Search results for "FISICA APPLICATA"
showing 10 items of 1318 documents
Integrated geophysical surveys in the tarragona cathedral
2009
An integrated geophysical survey has been conducted at the Tarragona’s Cathedral (Catalonia, NE Spain) with the aim to detect the existence of archaeological remains of the Roman’s temple devoted to August. Many hypotheses have been proposed about its possible location, the last ones regarding the inner part of the Cathedral, which is one of the most famous temple of Spain (12th century) evolving from Romanesque to Gothic styles. A project including electrical resistivity tomography (ERT), ground probing radar (GPR) and frequency domain electromagnetics (FDEM) has been planned over one year and conducted during a week of intensive field survey. From all the methods applied, both ERT and GPR…
The New "Maximum Yield Grid" (MYG) Array in Full 3D Resistivity Tomography
2008
Over the past few years geoelectrical prospecting has experienced many innovations, ranging from instrumental refinements to improvement of data interpretation. The consequence of this has been a strong increase in the method s resolution power and, therefore, its field of application has broadened. Nevertheless, the diffusion of 3D surveys in electrical resistivity tomography has been very much limited by the long acquisition time required by this kind of investigation, as compared to 2D ERT or other geophysical exploration methods like GPR. However, 3D ERT has a great advantage in terms of resolution power, that is very important in many applications, such as archeological or cultural her…
A Guide to Perform 3D Histology of Biological Tissues with Fluorescence Microscopy
2023
The analysis of histological alterations in all types of tissue is of primary importance in pathology for highly accurate and robust diagnosis. Recent advances in tissue clearing and fluorescence microscopy made the study of the anatomy of biological tissue possible in three dimensions. The combination of these techniques with classical hematoxylin and eosin (H&E) staining has led to the birth of three-dimensional (3D) histology. Here, we present an overview of the state-of-the-art methods, highlighting the optimal combinations of different clearing methods and advanced fluorescence microscopy techniques for the investigation of all types of biological tissues. We employed fluorescence …
Analysis of HVSR Data Using a Modified Centroid-Based Algorithm for Near-Surface Geological Reconstruction
2022
Recently, the use of microtremor techniques for subsoil investigation has increased significantly. The HVSR (Horizontal to Vertical Spectral Ratio) technique allows, in many cases, to obtain a seismo-stratigraphic reconstruction of the subsoil and to identify areas with similar seismic behavior. However, the stratigraphic interpretation of the HVSR peaks still remains a subjective choice and linked to a priori information. A non-hierarchical centroid-based algorithm was modified to group HVSR peaks of different measurements that can be attributed to the same generating seismic discontinuity. Some tests performed have shown that the proposed algorithm produces valid results even in the absen…
3D GPR Model in the Military District of San Giacomo Degli Spagnoli (Palermo)
2023
The georadar method was used to try to find some anthropic structures in a large square inside the Carabinieri barracks in the former military complex of San Giacomo degli Spagnoli in Palermo (Italy). These investigations are part of a broader context of a study of the entire area. The purpose of the investigations is to try to understand if under the ground there are the remains of an ancient horse passage that connected the Royal Palace of Palermo with the sea gate of the city. Furthermore, in the Middle Ages, on the site of the present square, there were most likely two churches, which no longer exist, as evidenced by numerous historical testimonies. One of the two, San Giacomo la Mazara…
3D nanometric particle tracking over tunable axial ranges
The precise localization of nanometric objects in three dimensions is essential to identify functional diffusion mechanisms in complex systems at the cellular or molecular level. However, most optical methods can achieve high temporal resolution and high localization precision only in two dimensions or over a limited axial range. Here we develop a novel wide-field detection system based on an electrically tunable lens that can track multiple individual nanoscale emitters in three dimensions over a tunable axial range with nanometric localization precision. The optical principle of the technique is based on the simultaneous acquisition of two images with an extended depth of field while enco…
Characterization of the Solid State Properties of Anodic Oxides on Magnetron Sputtered Ta, Nb and Ta-Nb Alloys
2012
Tantalum oxide, niobium oxide and Ta-Nb containing mixed oxides were grown by anodizing sputter-deposited Ta, Nb and Ta-Nb alloys of different compositions. A photoelectrochemical investigation was performed in order to estimate the band gap and the flat band potential of the oxides as a function of their composition. The band gap of the investigated Ta-Nb containing mixed oxides changed monotonically between those estimated for Ta2O5 (4.1 eV) and Nb2O5 (3.4 eV) and in agreement with a proposed correlation between the Band gap of an oxide and the difference of electronegativity of the oxide constituents. From the differential capacitance curves recorded in a wide range of electrode potentia…
In Silico Conformational Features of Botulinum Toxins A1 and E1 According to Intraluminal Acidification
2022
International audience; Although botulinum neurotoxins (BoNTs) are among the most toxic compounds found in nature, their molecular mechanism of action is far from being elucidated. A key event is the conformational transition due to acidification of the interior of synaptic vesicles, leading to translocation of the BoNT catalytic domain into the neuronal cytosol. To investigate these conformational variations, homology modeling and atomistic simulations are combined to explore the internal dynamics of the sub-types BoNT/A1 (the most-used sub-type in medical applications) and BoNT/E1 (the most kinetically efficient sub-type). This first simulation study of di-chain BoNTs in closed and open s…