Search results for "FMS"
showing 10 items of 44 documents
Biomethane Production from Anaerobic Co-Digestion of Selected Organic Fraction of Municipal Solid Waste (OFMSW) with Sewage Sludge: Effect of the Ino…
2021
The aim of this study was to evaluate the effect of the inoculum to substrate ratio (ISR) and the mixture ratio between organic fraction of municipal solid waste (OFMSW) and sewage sludge (SS) on the methane production potential achievable from anaerobic co-digestion (AcoD). Biochemical Methane Potential (BMP) assays at mesophilic temperature were used to determine the best AcoD configuration for maximizing methane yield and production rate, as well as to address possible synergistic effects. The maximum methane yield was observed at ISR of 1 and 60% OFMSW: 40% SS as co-digestion mixture, whereas the highest methane production rate was achieved at ISR of 2 with the same mixture ratio (207 m…
Role of SHP2 for FLT3-dependent proliferation and transformation in 32D cells.
2008
Fms-like tyrosine kinase 3 (FLT3) is a class III receptor tyrosine kinase, which plays a role in proliferation and differentiation of B-cell progenitors, myelomonocytic and dendritic cells, as well as in the maintenance of pluripotent hematopoietic stem cells (reviewed in Stirewalt and Radich,1and Schmidt-Arras et al.2). Recently, FLT3 has received much attention as an important oncoprotein. Mutations in FLT3 that lead to constitutive activation are among the most common molecular lesions found in acute myeloid leukemia.3 The most prevalent type of mutations result in internal tandem duplications (ITD) of amino-acid stretches in the juxtamembrane domain of FLT3. FLT3-ITD is constitutively a…
An Intelligent System for Decision Support in Bioinformatics
2011
The enormous array of computational techniques and data available due to today's use of high-throughput technologies can be quite overwhelming for researchers investigating biological problems. For any problem, there are many possible models and algorithms giving different results. We present a new Intelligent System that supports the selection, configuration and operation of strategies and tools in the bioinformatics domain. The Institute for High Performance Computing and Networking (ICAR-CNR) and the University of Palermo are developing an intelligent system that supports bioinformatics research. The system guides the researcher in building a data analysis workflow and acts as an interfa…
Assessment of organic waste management methods through energy balance
2014
In Italy, the Organic Fraction of Municipal Solid W aste (OFMSW) is nowadays landfilled or processed through aerobic composting. The Italian towns curre ntly support a high cost for OFMSW disposal and cause a high environmental impact, because of long distance s travelled from towns to a few available landfills and fewer treatment places, as well as the used waste m anagement methods. An interesting option for OFMSW is Anaerobic Digestion (AD), producing biogas and “digestate”. In this survey a theoretical biogas plant was placed near a town of Sicily Region (Italy), centra lised with reference to the area considered for pro ducing OFMSW. The distances travelled every year to transport OFMS…
A neoepitope generated by an FLT3 internal tandem duplication (FLT3-ITD) is recognized by leukemia-reactive autologous CD8+ T cells.
2006
Abstract The FLT3 receptor tyrosine kinase is expressed in more than 90% of acute myelogeneous leukemias (AMLs), up to 30% of which carry an internal tandem duplication (ITD) within the FLT3 gene. Although varying duplication sites exist, most FLT3-ITDs affect a single protein domain. We analyzed the FLT3-ITD of an AML patient for encoding HLA class I–restricted immunogenic peptides. One of the tested peptides (YVDFREYEYY) induced in vitro autologous T-cell responses restricted by HLA-A*0101 that were also detectable ex vivo. These peptide-reactive T cells recognized targets transfected with the patient's FLT3-ITD, but not wild-type FLT3, and recognized the patient's AML cells. Our results …
Effect of the MobilityWOD training program on functional movement patterns related to the risk of injury in CrossFit practitioners
2020
Background: CrossFit is an increasingly popular form of physical activity. However, due to its specificity and high intensity, it carries with it a considerable risk of injuries. So it is important to know if some specific training including exercises derived from physiotherapy and functional training (MobilityWOD) can reduce the risk of injury. Objective: The aim of the study is to assess the effect of MobilityWOD training program on functional movement patterns related to the risk of injury in adult male CrossFit practitioners. Methods: The study included 30 men aged 20-35 years old who practice CrossFit training five timeper week. Initially, a functional evaluation was performed using th…
Determination of phosphorylation degrees in caseins by on-line gel electrophoresis coupled to ICP-SFMS
2007
The determination of the phosphorylation degree of proteins is essential in many biochemical fields. Besides mass spectrometric techniques elucidating the protein structure, ICP-MS has entered this research area as it provides excellent quantification capabilities. In this work the separation of the proteins has been carried out by gel electrophoresis on-line coupled to an ICP equipped with a double-focusing sector field-MS (ELEMENT 2). Based on the simultaneous detection of 32S+ and 31P+, this work presents an alternative approach to determining the phosphorylation degree of caseins. For the example of α- and β-casein the results obtained were in excellent agreement with the expected value…
Consensus guidelines for the detection of immunogenic cell death
2014
Apoptotic cells have long been considered as intrinsically tolerogenic or unable to elicit immune responses specific for dead cell-associated antigens. However, multiple stimuli can trigger a functionally peculiar type of apoptotic demise that does not go unnoticed by the adaptive arm of the immune system, which we named "immunogenic cell death" (ICD). ICD is preceded or accompanied by the emission of a series of immunostimulatory damage-associated molecular patterns (DAMPs) in a precise spatiotemporal configuration. Several anticancer agents that have been successfully employed in the clinic for decades, including various chemotherapeutics and radiotherapy, can elicit ICD. Moreover, defect…
Economic and environmental sustainability of an AnMBR treating urban wastewater and organic fraction of municipal solid waste
2016
[EN] The objective of this study was to evaluate the economic and environmental sustainability of a sub- merged anaerobic membrane bioreactor (AnMBR) treating urban wastewater (UWW) and organic fraction of municipal solid waste (OFMSW) at ambient temperature in mild/hot climates. To this aim, power requirements, energy recovery from methane (biogas methane and methane dissolved in the effluent), consumption of reagents for membrane cleaning, and sludge handling (polyelectrolyte and energy consumption) and disposal (farmland, landfilling and incineration) were evaluated within different operating scenarios. Results showed that, for the operating conditions considered in this study, AnMBR tec…
AML-associated Flt3 kinase domain mutations show signal transduction differences compared with Flt3 ITD mutations
2005
Activating mutations of Flt3 are found in approximately one third of patients with acute myeloid leukemia (AML) and are an attractive drug target. Two classes of Flt3 mutations occur: internal tandem duplications (ITDs) in the juxtamembrane and point mutations in the tyrosine kinase domain (TKD). We and others have shown that Flt3-ITD induced aberrant signaling including strong activation of signal transducer and activator of transcription 5 (STAT5) and repression of CCAAT/estradiol-binding protein α (c/EBPα) and Pu.1. Here, we compared the signaling properties of Flt3-ITD versus Flt3-TKD in myeloid progenitor cells. We demonstrate that Flt3-TKD mutations induced autonomous growth of 32D ce…