Search results for "FOT"

showing 10 items of 1161 documents

Eksperimentāla mikroorganismu identifikācija punktātos, izmantojot mikrodaļiņas un spektrofotometru

2019

Ievads: Uz doto brīdi pastāv dažādas mikroorganismu diagnostiskas metodes, taču cilvēki joprojām cieš no infekcijas slimībām, kā arī mikroorganismu rezistence turpina augt. Tas liek aizdomāties par to, ka joprojām nav izdomāta ātra, lēta un maksimāli efektīva diagnostiskas metode. Jaunievedums – ir sen aizmirsts vecais. Spektrofotometrija ir sena metode, taču pagaidām netiek izmantota mikroorganismu atpazīšanai, kas dod iespēju pamēģināt uzlabot un renovēt to. Pētījuma mērķis ir noskaidrot mākslīgā bāzes punkāta absorbcijas pakāpi, pie kuras varētu identificēt mikroorganismus, izmantojot spektrofotometru un silīcija dioksīda mikrodaļiņas. Materiāli un metodes: Pētījumā tiek izmantotas 5 gra…

MCFARLAND VIENĪBASGAISMAS ABSORBCIJAMIKROORGANISMIMĀKSLĪGĀS BĀZES PUNKTĀTSMedicīnaSPEKTROFOTOMETRIJA
researchProduct

The three-dimensional structure of Drosophila melanogaster (6–4) photolyase at room temperature

2021

A crystal structure of a photolyase at room temperature confirms the structural information obtained from cryogenic crystallography and paves the way for time-resolved studies of the photolyase at an X-ray free-electron laser.

MECHANISMMaterials scienceAbsorption spectroscopyDNA repairfotobiologia02 engineering and technologyCrystal structureREPAIR ACTIVITY03 medical and health sciencesCOLI DNA PHOTOLYASEX-RAY-DIFFRACTIONCryptochromeStructural BiologyAnimalsserial crystallographyCRYSTAL-STRUCTURECRYPTOCHROMEPhotolyaseSERIAL FEMTOSECOND CRYSTALLOGRAPHY030304 developmental biology0303 health sciencesCrystallographyflavoproteinsFADResolution (electron density)TemperaturebanaanikärpänenDNAkidetiede(6-4) photolyase021001 nanoscience & nanotechnologyResearch PapersRADICAL TRANSFER(6–4) photolyaseroom-temperature structureCrystallographyphotolyasesDrosophila melanogasterRECONSTITUTIONX-ray crystallography1182 Biochemistry cell and molecular biologylämpötilaproteiinit0210 nano-technologyDeoxyribodipyrimidine Photo-LyasePHOTOACTIVATIONVisible spectrumActa Crystallographica Section D Structural Biology
researchProduct

Structural basis for light control of cell development revealed by crystal structures of a myxobacterial phytochrome

2018

Phytochromes are red-light photoreceptors that were first characterized in plants, with homologs in photosynthetic and non-photosynthetic bacteria known as bacteriophytochromes (BphPs). Upon absorption of light, BphPs interconvert between two states denoted Pr and Pfr with distinct absorption spectra in the red and far-red. They have recently been engineered as enzymatic photoswitches for fluorescent-marker applications in non-invasive tissue imaging of mammals. This article presents cryo- and room-temperature crystal structures of the unusual phytochrome from the non-photosynthetic myxobacterium Stigmatella aurantiaca (SaBphP1) and reveals its role in the fruiting-body formation of this ph…

MODULE0301 basic medicinePHOTOACTIVE YELLOW PROTEINSIGNALING MECHANISMabsorption spectraMutantfotobiologiaphytochromesBiochemistryyhteyttäminenbakteeritSTIGMATELLA-AURANTIACA03 medical and health sciencesFRUITING BODY FORMATIONGeneral Materials ScienceMolecular replacementStigmatella aurantiacalcsh:ScienceUNUSUAL BACTERIOPHYTOCHROMEPHOTOCONVERSIONHistidine030102 biochemistry & molecular biologybiologyPhytochromeChemistryCRYSTALLOGRAPHYta1182photosynthetic bacteriaphotoreceptorsGeneral ChemistryChromophoreCondensed Matter Physicsbiology.organism_classification030104 developmental biologyCHROMOPHORE-BINDING DOMAINBiophysicsmyxobacterialcsh:Q3111 BiomedicinePhotosynthetic bacteriaproteiinitMOLECULAR REPLACEMENTBinding domainIUCrJ
researchProduct

Kalcija ortofosfāta sintēzes apstākļu pētījumi

2015

Kalcija ortofosfāta sintēzes apstākļu pētījumi. Bezzaponnaja V., zinātniskais vadītājs Dr. chem., asoc. prof. Švirksts J. Bakalaura darbs, 48 lappuses, 19 tabulas, 26 attēli, 42 literatūras avoti, 1 pielikums. Latviešu valodā. Darbā apkopota informācija par dabīgajiem kalcija fosfātiem un hidroksīlapatītiem, to sintēzes un pētīšanas metodēm, katjonu aizvietošanu apatītos un fosfātos, kalcija fosfātu biomateriāliem. Darbā veikta kalcija ortofosfātu sintēze ar dažādu kalcija/fosfora molāro attiecību robežās no 1,20 līdz 1,60. Izmantojot kompleksonometriju un fotometriju noteikts kalcija un fosfora saturs sintezētajos paraugos. Paraugu fāzu noteikšanai pirms un pēc karsēšanas tika izmantota re…

MOLĀRĀ ATTIECĪBAFOTOMETRIJAKOMPLEKSONOMETRISKA TITRĒŠANAKALCIJA ORTOFOSFĀTIĶīmijaRENTGENDIFRAKTOMETRIJA
researchProduct

Synthesis of Soluble Donor-Acceptor Double-Cable polymers based on polythiophene and tetracyanoanthraquinodimethane (TCAQ).

2003

[structure: see text] Novel suitably functionalized tetracyanoanthraquinodimethane (TCAQ) derivatives covalently linked to thiophene moieties have been synthesized. The thiophene-based monomers have been chemically polymerized and copolymerized to yield new and soluble donor-acceptor double-cable polymers. The absorption and emission data reveal that the optical properties can be finely tuned by modifying the ratio of monomers in the copolymerization process.

Magnetic Resonance SpectroscopyFotoricettoriPolymersAnthraquinonesThiophenesmacromolecular substancesBiochemistryIndicators and Reagentchemistry.chemical_compoundSpettroscopiaThiophenePolymer chemistryCopolymerThiophenePhysical and Theoretical ChemistryPolymerPolimerichemistry.chemical_classificationOrganic Chemistrytechnology industry and agriculturePolymerSettore CHIM/06 - Chimica OrganicaMonomerchemistryPolymerizationCovalent bondLuminescent MeasurementLuminescent MeasurementsPolythiopheneMolecular MedicineAnthraquinoneIndicators and ReagentsSpectrophotometry UltravioletAbsorption (chemistry)Celle solariSintesi
researchProduct

Genome-wide Association Studies Identify Genetic Loci Associated with Albuminuria in Diabetes

2016

Elevated concentrations of albumin in the urine, albuminuria, are a hallmark of diabetic kidney disease and are associated with an increased risk for end-stage renal disease and cardiovascular events. To gain insight into the pathophysiological mechanisms underlying albuminuria, we conducted meta-analyses of genome-wide association studies and independent replication in up to 5,825 individuals of European ancestry with diabetes and up to 46,061 without diabetes, followed by functional studies. Known associations of variants in CUBN, encoding cubilin, with the urinary albumin-to-creatinine ratio (UACR) were confirmed in the overall sample (P = 2.4 × 10−10). Gene-by-diabetes interactions were…

Male0301 basic medicinediabetes geneEndocrinology Diabetes and MetabolismGenome-wide association studyKidneyGLOMERULAR-FILTRATION-RATECathepsin CGene Knockout TechniquescubilinSettore MED/14 - NEFROLOGIADiabetic NephropathiesMODULATES PROTEINURIAddc:616HERITABILITYDiabetesGenetics/Genomes/Proteomics/MetabolomicsMiddle AgedRISK POPULATION COHORTS3. Good healthINSIGHTSKidney TubulesFemaleSulfotransferasesmedicine.symptomRAB38AdultEXPRESSIONmedicine.medical_specialtyRenal functionReceptors Cell Surface610 Medicine & healthSingle-nucleotide polymorphismBiologyPolymorphism Single NucleotidealbuminuriaDiabetes Mellitus Experimental03 medical and health sciencesDiabetes mellitusInternal medicineInternal MedicinemedicineAnimalsHumansGenetic Predisposition to DiseaseAgedMORTALITYKIDNEY-DISEASEmedicine.diseaseCubilinRatsMinor allele frequency030104 developmental biologyEndocrinologyDiabetes Mellitus Type 2rab GTP-Binding ProteinsCOLLABORATIVE METAANALYSISAlbuminuria570 Life sciences; biologyalbuminuria diabetes cubilinGenome-Wide Association StudyKidney disease
researchProduct

THE DISTRIBUTION OF UDP-GLUCURONOSYLTRANSFERASES IN RAT-LIVER PARENCHYMAL AND NONPARENCHYMAL CELLS

1992

Activities for the glucuronidation of 1-naphthol, morphine and bilirubin as well as for the sulfation of 2-naphthol have been determined in homogenates of parenchymal, Kupffer and endothelial cells isolated from livers of untreated and Aroclor 1254-pretreated rats. In addition, Western blot analyses using different polyclonal antibodies against UDP-glucuronosyltransferases (UDP-GTs) were performed with similar preparations. All enzymes under investigation were expressed at high levels in liver parenchymal cells. The constitutive expression and inducibility of UDP-GT isozyme(s) for 1-naphthol glucuronidation was also clearly demonstrated in Kupffer and endothelial cells. Furthermore, the pre…

MaleAroclorsCell type1303 BiochemistryKupffer CellsLiver cytologyBilirubinBlotting WesternGlucuronidation10050 Institute of Pharmacology and Toxicology610 Medicine & healthCell SeparationBiologyBiochemistryIsozymechemistry.chemical_compoundSulfationmedicineAnimalsEndotheliumGlucuronosyltransferasePharmacologyKupffer cellRats Inbred StrainsChlorodiphenyl (54% Chlorine)ArylsulfotransferaseMolecular biologyRatsIsoenzymesEndothelial stem cellmedicine.anatomical_structure3004 PharmacologyLiverchemistryBiochemistry570 Life sciences; biology
researchProduct

Effects of typical inducers on olfactory xenobiotic-metabolizing enzyme, transporter, and transcription factor expression in rats.

2010

International audience; Several xenobiotic-metabolizing enzymes (XMEs) have been identified in the olfactory mucosa (OM) of mammals. However, the molecular mechanisms underlying the regulation of these enzymes have been little explored. In particular, information on the expression of the transcriptional factors in this tissue is quite limited. The aim of the present study was to examine the impact of five typical inducers, Aroclor 1254, 3-methylcholanthrene, dexamethasone, phenobarbital, and ethoxyquin, on the activities and mRNA expression of several XMEs in the OM and in the liver of rats. We also evaluated the effects of these treatments on the mRNA expression of transcription factors an…

MaleLIVERMESH : Transcription FactorsMESH: Microsomes Liver[ SDV.AEN ] Life Sciences [q-bio]/Food and NutritionPharmaceutical ScienceMESH : CytochromesMESH: Down-RegulationMESH: Membrane Transport ProteinsMESH : Down-RegulationCytosol0302 clinical medicineGlucocorticoid receptorMESH : Membrane Transport ProteinsMESH: CytosolMESH: Reverse Transcriptase Polymerase Chain ReactionGene expressionConstitutive androstane receptorMESH: Up-RegulationMESH: AnimalsReceptorMESH : Up-RegulationMESH: Cytochromes0303 health sciencesPregnane X receptorMESH : Metabolic Detoxication Phase IbiologyReverse Transcriptase Polymerase Chain ReactionMESH : RatsMESH : CytosolINDUCTIONMESH : Reverse Transcriptase Polymerase Chain ReactionMESH: Transcription FactorsUp-Regulation3. Good healthMESH : Microsomes LiverHYDROCARBON HYDROXYLASE-ACTIVITYmedicine.anatomical_structurePHASE-IBiochemistryMESH: Metabolic Detoxication Phase IIEnzyme InductionMicrosomes LiverMESH: Metabolic Detoxication Phase IMESH: XenobioticsMESH: Enzyme InductionMESH: RatsMESH : MaleDown-RegulationMESH : XenobioticsPHENOL SULFOTRANSFERASEMESH : Rats WistarXenobiotics03 medical and health sciencesOlfactory mucosaOlfactory MucosamedicineAnimalsRats WistarMESH: Olfactory MucosaTranscription factor030304 developmental biologyPharmacologyMESH : Olfactory MucosaIDENTIFICATIONRECEPTORMESH : Enzyme InductionMembrane Transport ProteinsMESH : Metabolic Detoxication Phase IIUDP-GLUCURONOSYLTRANSFERASEMESH: Rats WistarAryl hydrocarbon receptorORGANIC ANION TRANSPORTERMolecular biologyMetabolic Detoxication Phase IIMESH: MaleRatsNASAL-MUCOSAbiology.proteinCytochromesMetabolic Detoxication Phase IMESH : Animals[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition030217 neurology & neurosurgeryTranscription Factors
researchProduct

Evaluation of effectiveness of photobiostimulation in alleviating side effects after dental implant surgery. A randomized clinical trial

2020

Background To assess if photobiostimulation (PBS) alleviates pain intensity/duration and swelling after implant surgery. Material and Methods Sixty subjects (27 male and 33 female, with a mean age of 47,13 8.05 years) were included and randomly assigned to experimental group (implant surgery and photobiostimulation), placebo group (implant surgery and simulated photobiostimulation) and control group (implant surgery only). Inclusion criteria: subjects older than 20 years, with a healthy oral mucosa and requiring implant surgery. Exclusion criteria: pregnancy, history of implant failure, light sensitivity, metabolic deseases, consumption of antibiotics or corticosteroids in the last two week…

MaleOral Surgical ProceduresAnalgesicCirurgia dentalPainPlaceboPhotobiostimulationImplant surgerylaw.invention03 medical and health sciences0302 clinical medicineDouble-Blind MethodRandomized controlled trialInterquartile rangelawmedicineHumansOral mucosaGeneral DentistryAged 80 and overDental ImplantsLLLT photobiostimulation pain implant surgery.AnalgesicsPain PostoperativePregnancyImplants dentalsbusiness.industryLLLTResearchDental implantsImplant failureMED/28 - MALATTIE ODONTOSTOMATOLOGICHE030206 dentistrymedicine.disease:CIENCIAS MÉDICAS [UNESCO]Photobiologymedicine.anatomical_structureFotobiologiaOtorhinolaryngologyAnesthesiaDental surgeryUNESCO::CIENCIAS MÉDICASFemaleSurgeryAnalysis of varianceLLLT Photobiostimulation Pain Implant surgerybusinessImplantology
researchProduct

Serial measurements of transient evoked otoacoustic emissions (TEOAEs) in healthy newborns and in newborns with perinatal infection.

1993

Detection of hearing impairment in early childhood is difficult. We serially recorded transient evoked otoacoustic emissions (TEOAEs) to search for signs of ototoxicity in term, healthy newborns and compared the results to a second group of term babies treated for perinatally acquired bacterial infection with ampicillin plus either cefotaxime or plus aminoglycoside. At initial evaluation, in the group of 45 healthy children born at term, well reproducible emissions were observed in all but two children. In each of these two, initially well reproducible TEOAEs were detected in one ear only. At the time of the second recording (mean at day 8.5) excellent emissions were seen in all ears of all…

MalePediatricsmedicine.medical_specialtyCefotaximeOtoacoustic emissionCefotaximeAudiologyOtotoxicityAmpicillinmedicineTobramycinHumansNetilmicinCochleabusiness.industryAminoglycosideInfant NewbornReproducibility of ResultsGeneral MedicineBacterial Infectionsmedicine.diseaseReflex AcousticAnti-Bacterial AgentsCochleaOtorhinolaryngologyPediatrics Perinatology and Child HealthAuditory PerceptionEvoked Potentials AuditoryTobramycinAmpicillinFemaleNetilmicinbusinessmedicine.drugFollow-Up StudiesInternational journal of pediatric otorhinolaryngology
researchProduct