Search results for "FRET"

Wild-type Cu/Zn superoxide dismutase (SOD1) does not facilitate, but impedes the formation of protein aggregates of amyotrophic lateral sclerosis cau…

2009

Aggregation of Cu/Zn superoxide dismutase (SOD1) is a hallmark of a subset of familial amyotrophic lateral sclerosis (ALS) cases. The expression of wild-type SOD1 [SOD(hWT)] surprisingly exacerbates the phenotype of mutant SOD1 in vivo. Here we studied whether SOD1(hWT) may affect mutant SOD1 aggregation by employing fluorescence microscopy techniques combined with lifetime-based Förster resonance energy transfer (FRET). Only a very minor fraction of SOD1(hWT) was observed in aggregates induced by mutant SOD1(G37R), SOD1(G85R) or SOD1(G93C). Quite in contrast, co-expression of SOD(hWT) reduced the amount of mutant SOD1 in the aggregate fraction. Furthermore, we did not detect endogenous mou…

A TR-FRET based functional assay for screening activators of CARM1

2013

Epigenome is an emerging field that demands selective cell-permeable chemical probes to perturb, especially in vivo, the activity of specific enzymes involved in modulating the epigenetic codes. Coactivator Associated Arginine (R) Methyltransferase 1 (CARM1) is a coactivator of estrogen receptor α (ERα), the main target in human breast cancer. We previously showed that overexpression of CARM1 by two-fold in MCF7 breast cancer cells increased the expression of ERα-target genes involved in differentiation and reduced cell proliferation, leading to the hypothesis that activating CARM1 by chemical activators may be therapeutically effective in breast cancer. Selective, potent, cell-permeable CA…

Single-molecule FRET studies of counterion effects on the free energy landscape of human mitochondrial lysine tRNA.

2011

The folding energy landscape of RNA is greatly affected by interactions between the RNA and counterions that neutralize the backbone negative charges and may also participate in tertiary contacts. Valence, size, coordination number, and electron shell structure can all contribute to the energetic stabilization of specific RNA conformations. Using single-molecule fluorescence resonance energy transfer (smFRET), we have examined the folding properties of the RNA transcript of human mitochondrial tRNA(Lys), which possesses two different folded states in addition to the unfolded one under conditions of thermodynamic equilibrium. We have quantitatively analyzed the degree of RNA tertiary structu…

Specific binding of VegT mRNA localization signal to membranes in Xenopus oocytes

2021

Abstract We have studied the interaction of a VegT mRNA localization signal sequence with the membranes of the mitochondrial cloud in Xenopus oocytes, and the binding of the VegT mRNA signal sequence to the lipid raft regions of the vesicles bounded by ordered and disordered phospholipid bilayers. RNA preference for the membranes of the mitochondrial cloud was confirmed using microscopy of a fluorescence resonance energy transfer from RNA molecules to membranes. Our studies show that VegT mRNA has a higher affinity for ordered regions of lipid bilayers. This conclusion is supported by the dissociation constant measurements for RNA-liposome complex and the visualization of the FRET signal be…

José Vidal-Beneyto/Autor de "Memoria democrática"/Entrevista. "Estamos empantanados en la corrupción"

2007

LA QUALITÉ ET LA VALEUR PERÇUE DANS LE TRANSPORT DE MARCHANDISES EN ESPAGNE ET LEUR IMPORTANCE DANS LA SEGMENTATION DE LA CLIENTÈLE

2015

En el estudio académico de las actividades logísticas y específicamente del transporte de mercancías, se ha señalado la influencia de la calidad del servicio en la generación y percepción de valor, así como la importancia de estos dos constructos en el proceso de evaluación del servicio en este entorno particular. En este trabajo se pretende identificar y caracterizar segmentos de empresas que prestan servicios de gestión del transporte internacional de mercancías (transitarias), con base en el valor que perciben del servicio recibido de sus principales proveedores de transporte de mercancías. A través de la realización de un análisis factorial exploratorio sobre una muestra de 205 empresas…

CO2-ECHO Quantification des émissions de CO2 du transport de fret à partir de la base ECHO

2014

Chemins de fer et cheminots en tension

2018

International audience; La SNCF polarise régulièrement l'actualité économique, sociale et politique. Elle a connu en 2018 une nouvelle réforme qui l'a plongée dans plusieurs mos de crise. Plus largement, c'est l'ensemble des chemins de fer européens qui ont fait l'objet de réformes. Associant universitaires, consultats et syndicalistes, ce livre fait le point sur les évolutions récentes de ce mode de transport dans différents pays : Allemagne, Espagne, France, Italie, Royaume-Uni, Suisse. Il s'intéresse aux modalités d'ouverture à la concurrence et à leurs résultats, à l'économie et aux performances des compagnies, au devenir et aux enjeux du fret, aux statuts des personnels, au management …

Fonctionnalisation de surfaces métalliques par des couches minces d'oxynitrures de titane obtenues par irradiation laser sous atmosphère contrôlée et…

2013

The technological advances in areas such as automotive and aerospace industries require materials capable of withstanding demanding conditions. Surface treatments are effective tools to improve the surface properties of materials while preserving their bulk properties. Besides many conventional processes, laser treatments are efficient methods for hardening and increasing the durability of materials. This work is focused on the growth of titanium oxynitride surface layers by laser irradiation of pure titanium plates with nanosecond pulsed sources. The influence of the laser treatment conditions on the composition and the structure of these surface layers was studied. In particular, for lase…

Jaunu Staphylococcus Epidermidis sortāzes A inhibitoru atlase izmantojot FRET skrīninga sistēmu

2020

Pētījuma mērķis ir atrast jaunus zāļu kandidātus, kuri spēj inhibēt Staphylococcus epidermidis sortāzi A, vienlaicīgi mazinot patogēnu multirezistēnces izplatīšanos. Šim mērķim tika izveidoti ģenētiski DNS konstrukti, kuri kodē S.epidermidis sortāzes A katalītisko domēnu, iebūvējot pET22b un pET28a vektoros srtA gēnu. Rekombinantā sortāzes proteīna sintēze tika inducēta ar IPTG, izmantojot BL21(DE3) celmu Escherichia coli ekspresijas sistēmā. Proteīns bez papildus heksahistidīna sekvences (pET22b vektors) tika attīrīts ar anjonapmaiņas hromatogrāfijas kolonnām, savukārt heksahistidīna sekvenci saturošā sortāze (pET28a vektors) tika attīrīta iz…