Search results for "Factory"
showing 10 items of 490 documents
Novel NG2-CreERT2 knock-in mice demonstrate heterogeneous differentiation potential of NG2 glia during development
2014
NG2 (nerve/glia antigen-2) is a type I transmembrane glycoprotein and also known as chondroitin sulfate proteoglycan 4. In the parenchyma of the central nervous system, NG2-expressing (NG2(+) ) cells have been identified as a novel type of glia with a strong potential to generate oligodendrocytes (OLs) in the developing white matter. However, the differentiation potential of NG2 glia remained controversial, largely attributable to shortcomings of transgenic mouse models used for fate mapping. To minimize these restrictions and to more faithfully mimic the endogenous NG2 expression in vivo, we generated a mouse line in which the open reading frame of the tamoxifen-inducible form of the Cre D…
Fatal neuroinvasion and SARS-CoV-2 tropism in K18-hACE2 mice is partially independent on hACE2 expression
2022
ABSTRACTAnimal models recapitulating distinctive features of severe COVID-19 are critical to enhance our understanding of SARS-CoV-2 pathogenesis. Transgenic mice expressing human angiotensin-converting enzyme 2 (hACE2) under the cytokeratin 18 promoter (K18-hACE2) represent a lethal model of SARS-CoV-2 infection. The precise mechanisms of lethality in this mouse model remain unclear. Here, we evaluated the spatiotemporal dynamics of SARS-CoV-2 infection for up to 14 days post-infection. Despite infection and moderate pneumonia, rapid clinical decline or death of mice was invariably associated with viral neuroinvasion and direct neuronal injury (including brain and spinal neurons). Neuroinv…
7E olfactory receptor gene clusters and evolutionary chromosome rearrangements
2005
Olfactory receptor (OR) genes of the 7E subfamily have been duplicated to multiple regions throughout the human genome. Segmental duplications containing 7E OR genes have been associated with both pathological and evolutionary chromosome rearrangements. Many of these breakpoint regions coincide with breaks of chromosomal synteny in the mouse, rat and/or chicken genomes. Collectively, these data suggest that 7E OR-containing regions represent hot spots of genomic instability.
Zebrafish Reveals Different and Conserved Features of Vertebrate Neuroglobin Gene Structure, Expression Pattern, and Ligand Binding
2004
Neuroglobin has been identified as a respiratory protein that is primarily expressed in the mammalian nervous system. Here we present the first detailed analysis of neuroglobin from a non-mammalian vertebrate, the zebrafish Danio rerio. The zebrafish neuroglobin gene reveals a mammalian-type exon-intron pattern in the coding region (B12.2, E11.0, and G7.0), plus an additional 5'-non-coding exon. Similar to the mammalian neuroglobin, the zebrafish protein displays a hexacoordinate deoxy-binding scheme. Flash photolysis kinetics show the competitive binding on the millisecond timescale of external ligands and the distal histidine, resulting in an oxygen affinity of 1 torr. Western blotting, i…
Adult Neurogenesis in Reptiles
2011
Adult neurogenesis in reptiles is a well-documented phenomenon and exists in many telencephalic areas. The newly generated neurons originate along the walls of the lateral ventricles, mainly in the sulci. The putative neural progenitors are radial glial cells. These glial cells give rise to neuroblasts that migrate to their final destination. In general, the new neurons are born in the portion of the ventricular zone (VZ) adjacent to the telencephalic area where they will be recruited and migrate radially through the brain parenchyma along the processes of radial glial cells to their final destination, although migration to the olfactory bulbs (OB) is different. Specifically, it seems that …
Dendritic pattern development of the honeybee antennal lobe neurons: a laser scanning confocal microscopic study
1999
The processing of odorant signals is performed, in the olfactory bulb of vertebrates or in the antennal lobe of insects, by different types of neurons which display specific morphological and functional features. The present work characterizes the morphogenesis of the main neuronal types which participate in olfactory discrimination in the adult honeybee (Apis mellifera). Neurons were stained intracellularly with Lucifer yellow at different stages of pupal development and in the adult, and imaged by laser scanning confocal microscopy. Attending to branching patterns, all pupal neurons could be attributed to morphological types previously established in the adult. Given the functional import…
I poteri di Gramsci: Il dibattito con Bordiga tradotto e (ri-)pensato da Gérard Granel
2017
Questo contributo propone un close reading del saggio Gramsci et le pouvoir, pubblicato nel 1990, nel quale il pensatore e traduttore francese Gérard Granel analizza e commenta il dibattito tra Gramsci e Bordiga sulla questione dei consigli di fabbrica. Granel esamina il legame rispettivo dei due autori italiani al pensiero marxiano e rivela in che misura la “traduzione gramsciana” di concetti chiave come politica, proletariato e comunismo coglie il potenziale rivoluzionario dell’“evento Marx” al di là delle sue strumentalizzazioni ideologiche nei marxismi storici. This article is a close-reading of the essay Gramsci et le pouvoir, published in 1990 by the French thinker and translator Géra…
Ligand binding study revealing a human olfactory receptor involved in waxy-floral odor perception
2006
International audience
3D-QSAR study of ligands for a human olfactory receptor
2005
Abstract Only about 350 olfactory receptors (OR) provide a basis for the remarkable ability of humans to recognise and discriminate a large number of odorants. A recent study reports the odorant repertoire of a human class II OR called OR1G1, including both agonists and antagonists. We used these affinity data to perform a 3D molecular modelling study of these ligands using Catalyst/HypoGen software (Catalyst v4.9.1, Accelrys Inc., San Diego, 2004) to propose alignment models for OR1G1 ligands. We obtained a triple-alignment model, which satisfactorily explained the experimental activities and was able both to predict the antagonist effects of some compounds and to identify new potent agoni…
A human olfactory receptor for waxy, fatty and rose odors
2008
International audience