Search results for "Fluorescence microscope"

Epifluorescence microscopy studies of fatty acid monolayers: Domain formation induced by polymeric gegenions

1991

The monolayer behavior of long-chain fatty acids at the air/water interface on a poly(ethyleneimine) containing subphase was investigated. The interaction of the polymeric gegenions in the subphase leads to expanded states and permits the visualization of the aggregation behavior of different long-chain fatty acids via fluorescence microscopy. The monolayer morphologies and their variability were intensively studied by fluorescence microscopy. In addition, the transfer of the complexed fatty acid monolayers to solid substrates has been investigated.

Intravital fluorescence microscopy for the study of leukocyte interaction with platelets and endothelial cells

1999

Publisher Summary This chapter introduces the techniques of intravital microscopy as a tool to study the microcirculation in intact animals under conditions of oxidative stress. Intravital fluorescence microscopy can be performed with almost all types of epiillumination microscopes available. The introduction of fluorescent dyes and refined epiillumination techniques has significantly advanced the possibilities of intravital microscopic studies of the liver microcirculation, including quantitative analyses of both circulatory parameters and cellular mechanisms. Using appropriate dyes, intravital microscopy allows the study of (1) microvascular perfusion, (2) leukocyte-endothelial cell inter…

Watching the photo-oxidation of a single aromatic hydrocarbon molecule

2001

International audience; The photooxidation of single dye molecules (see scheme) can be followed by confocal fluorescence microscopy. The self-sensitized reaction with singlet oxygen leads to a suite of products, which may be differentiated spectrally. Tentative structures for certain photoproducts have been obtained from quantum-chemical calculations.

Investigations of the Microstructure of Lipid Interface Films

1990

The phase behaviour of phospholipid monolayers at electrolyte/gas interfaces is studied by fluorescence microscopy. At the LE/LC phase transition, phase separation leads to a Wigner-type lattice structure. The observations are quantified using digital image processing. The results show that the phase transition comprises three different regimes.

Bio-orthogonal Red and Far-Red Fluorogenic Probes for Wash-Free Live-Cell and Super-resolution Microscopy

2021

Small-molecule fluorophores enable the observation of biomolecules in their native context with fluorescence microscopy. Specific labeling via bio-orthogonal tetrazine chemistry combines minimal label size with rapid labeling kinetics. At the same time, fluorogenic tetrazine–dye conjugates exhibit efficient quenching of dyes prior to target binding. However, live-cell compatible long-wavelength fluorophores with strong fluorogenicity have been difficult to realize. Here, we report close proximity tetrazine–dye conjugates with minimal distance between tetrazine and the fluorophore. Two synthetic routes give access to a series of cell-permeable and -impermeable dyes including highly fluorogen…

Microneedling dilates the follicular infundibulum and increases transfollicular absorption of liposomal sepia melanin

2015

Gabriel Serrano,1 Patricia Almudéver,2 Juan M Serrano,3 Julio Cortijo,2 Carmen Faus,1 Magda Reyes,1 Inmaculada Expósito,3 Ana Torrens,3 Fernando Millán1 1Clínica Dermatológica Serrano, 2Department of Pharmacology, University of Valencia, 3Research and Development Department, Sesderma Laboratories, Valencia, Spain Abstract: Encapsulation of chemicals in liposomes and microneedling are currently used techniques to enhance the penetration of several substances through skin and hair. In this study, we apply a liposomal melanin–fluorescein compound to an ex vivo model of human skin, using a new electrical microneedling device (Nano…

2019

Ultrasmall polyaminocarboxylate-coated gold nanoparticles (NPs), Au@DTDTPA and Au@TADOTAGA, that have been recently developed exhibit a promising potential for image-guided radiotherapy. In order to render the radiosensitizing effect of these gold nanoparticles even more efficient, the study of their localization in cells is required to better understand the relation between the radiosensitizing properties of the agents and their localization in cells and in tumors. To achieve this goal, post-functionalization of Au@DTDTPA nanoparticles by near-infrared (NIF) organic dyes (aminated derivative of cyanine 5, Cy5-NH2) was performed. The immobilization of organic Cy5-NH2 dyes onto the gold nano…