Search results for "Fluorescent"
showing 10 items of 863 documents
Neuropeptide Y (NPY)-like immunoreactivity in the guinea pig pineal organ.
1986
Relatively little is known about mammalian pineal neuropeptides. In the present study neuropeptide Y-like immunoreactivity (NPY-LI) was examined in the guinea pig pineal gland. NPY-LI was restricted to few intrapineal nerve fibers of faint fluorescence intensity. They showed no preferential localization with regard to the different pineal portions. As catecholaminergic fibers are abundant in the guinea pig pineal gland, the scarcity of NPY-LI fibers indicates that in the pineal colocalization of noradrenaline and NPY-LI is not a regular feature, in contrast to other organs. The possibility exists that in the pineal NPY-LI fibers are not of peripheral sympathetic but of central origin.
Influence of nerve growth factor in endometriosis-associated symptoms
2011
To investigate the role of the nerve growth factor (NGF) in the development of dysmenorrhea/pelvic pain in patients with endometriosis, we performed a prospective, clinical, blind study. Peritoneal fluids (PFs) were obtained from patients with histologically proven endometriosis. Patients with endometriosis were divided into 7 different groups depending on their preoperative pain score and symptomatology: patients with no pain, patients with minimal pain (dysmenorrhea, pelvic pain, or both), and patients with severe pain (dysmenorrhea, pelvic pain, or both) and were used for the neuronal growth assay with cultured chicken dorsal root ganglia (DRG) and for Western blot analyses. Dorsal root …
On dynamics of parvoviral replication protein NS1
2010
Fluorescent naphthalimide-imidazolium hydrogels for biomedical applications
2019
Bioimaging and in vivo imaging are cornerstone technologies in support of biomedical diagnosis. However, in some cases imaging methods have increased cancer risks for patients. Moreover, the most widely used diagnostic medical imaging technique, X-ray imaging, is the largest man-made source of radiation exposure to the general population. Thus, the research of new efficient and less invasive materials for imaging is quite urgent. Supramolecular hydrogels have recently proved to be promising biological carriers to load versatile bioimaging agents for in vitro or in vivo bioimaging, thanks to the ability to undergo reversible swelling and gel–sol transition in response to various physiologica…
Supramolecular Association of Halochromic Switches and Halloysite Nanotubes in Fluorescent Nanoprobes for Tumor Detection
2022
Fluorescence imaging has become an indispensable tool in the biomedical laboratory to elucidate the fundamental dynamic and structural factors regulating cellular processes. The development of fluorescent nanoprobes represents a challenge to detect any cellular process under a microscope. Herein, a fluorescent nanomaterial was synthesized by exploiting the supramolecular interaction between a halochromic switch (1Cl) and halloysite nanotubes (HNTs). The successful synthesis of a HNTs/1Cl nanomaterial was confirmed by thermogravimetric analysis and Fourier transform infrared. The aqueous mobility was investigated by dynamic light scattering and ζ-potential measurements as well. Furthermore, …
Citoģenētiskas abnormalitātes pacientiem ar hronisko limfoleikozi
2022
Problēmas būtība. Hroniskā limfoleikoze ir visbiežāk sastopama leikoze pieaugušo populācijā. Tā raksturojas ar heterogēnu slimības gaitu un progresijas ātrumu. Mūsdienās slimības prognoze tiek noteikta pēc Rai un Binet klasifikāciju stadijām, taču šīs klasifikācijas nesniedz informāciju par slimības progresijas ātrumu. Šī iemesla dēļ mūsdienās tiek pētīti pacientu individuāli riska faktori, kas palīdzētu identificēt pacientu grupas ar agresīvāku slimības gaitu un tos, kam ir nepieciešama agrīna specifiskas terapijas uzsākšana. Pētījuma mērķis. Izpētīt HLL pacientu citoģenētiskas abnormalitātes un to saistību ar slimības stadiju un klīniskiem slimības simptomiem. Metodes un materiāli. Tika a…
Light-dependent CK2-mediated phosphorylation of centrins regulates complex formation with visual G-protein.
2008
AbstractCentrins are Ca2+-binding EF-hand proteins. All four known centrin isoforms are expressed in the ciliary apparatus of photoreceptor cells. Cen1p and Cen2p bind to the visual G-protein transducin in a strictly Ca2+-dependent way, which is thought to regulate light driven movements of transducin between photoreceptor cell compartments. These relatively slow motile processes represent a novel paradigm in light adaptation of photoreceptor cells.Here we validated specific phosphorylation as a novel regulator of centrins in photoreceptors. Centrins were differentially phosphorylated during photoreceptor dark adaptation. Inhibitor treatments revealed protein kinase CK2 as the major protein…
Resonance properties of GABAergic interneurons in immature GAD67-GFP mouse neocortex.
2014
Subthreshold resonance is a characteristic membrane property of different neuronal classes, is critically involved in the generation of network oscillations, and tunes the integration of synaptic inputs to particular frequency ranges. In order to investigate whether neocortical GABAergic interneurons show resonant behavior already during early postnatal development, we performed whole-cell patch-clamp recordings from visually identified interneurons in supragranular layers of parietal regions in coronal neocortical slices from postnatal day (P) P6-P13 GAD67-GFP knock-in mice. Subthreshold resonance was analyzed by injection of sinusoidal current with varying frequency. About 50% of the inve…
Using exomarkers to assess mitochondrial reactive species in vivo
2014
Background:\ud The ability to measure the concentrations of small damaging and signalling molecules such as reactive oxygen species (ROS) in vivo is essential to understanding their biological roles. While a range of methods can be applied to in vitro systems, measuring the levels and relative changes in reactive species in vivo is challenging.\ud \ud Scope of review:\ud One approach towards achieving this goal is the use of exomarkers. In this, exogenous probe compounds are administered to the intact organism and are then transformed by the reactive molecules in vivo to produce a diagnostic exomarker. The exomarker and the precursor probe can be analysed ex vivo to infer the identity and a…