Influence of nerve growth factor in endometriosis-associated symptoms

6533b837fe1ef96bd12a1f3d

RESEARCH PRODUCT

Influence of nerve growth factor in endometriosis-associated symptoms

Andreas D. Ebert Vito Chiantera Sylvia Mechsner Achim Schneider Maria Luisa Barcena De Arellano Giuseppe Filiberto Vercellino Julia Arnold

subject

endometriosis Endometriosis Fluorescent Antibody Technique Chick Embryo Gastroenterology pain generation of endometriosis-related symptom Severity of Illness Index endometriosis; pelvic pain; sensory nerve fibers GAP-43 Protein Ganglia Spinal Germany Nerve Growth Factor Ascitic Fluid Prospective Studies Endometriosi Prospective cohort study Cells Cultured Pain Measurement Neurons biology medicine.diagnostic_test Obstetrics and Gynecology pelvic pain Middle Aged Blot Anesthesia Female medicine.symptom Neurotrophin Human Adult medicine.medical_specialty Calcitonin Gene-Related Peptide Blotting Western sensory nerve fibers Calcitonin gene-related peptide sensory nerve fiber Young Adult Western blot Dysmenorrhea Internal medicine medicine Animals Humans business.industry Animal Pelvic pain Neuron medicine.disease Settore MED/40 - Ginecologia E Ostetricia Prospective Studie Nerve growth factor biology.protein business

description

To investigate the role of the nerve growth factor (NGF) in the development of dysmenorrhea/pelvic pain in patients with endometriosis, we performed a prospective, clinical, blind study. Peritoneal fluids (PFs) were obtained from patients with histologically proven endometriosis. Patients with endometriosis were divided into 7 different groups depending on their preoperative pain score and symptomatology: patients with no pain, patients with minimal pain (dysmenorrhea, pelvic pain, or both), and patients with severe pain (dysmenorrhea, pelvic pain, or both) and were used for the neuronal growth assay with cultured chicken dorsal root ganglia (DRG) and for Western blot analyses. Dorsal root ganglia were stained with anti-calcitonin gene-related peptide (CGRP) and anti-growth-associated protein 43 (GAP 43). Peritoneal fluids from patients with endometriosis induce neurite outgrowth. There was no significant difference in the outgrowth between the 7 pain groups. Western blot analyses showed a moderate NGF expression in the PFs from patients with endometriosis, without significant differences in the 7 pain groups. The present study suggests that the neurotrophic properties of endometriotic tissues are endometriosis- and not pain-associated. © Society for Gynecologic Investigation 2011.

year	journal	country	edition	language
2011-01-01

10.1177/1933719111410711 http://hdl.handle.net/11695/2733