0000000000188332

AUTHOR

Maria Luisa Barcena De Arellano

showing 8 related works from this author

Immunohistochemical characterization of endometriosis-associated smooth muscle cells in human peritoneal endometriotic lesions

2011

Background Smooth muscle cells (SMC) are common components of endometriotic lesions. SMC have been characterized previously in peritoneal, ovarian and deep infiltrating endometriotic lesions and adenomyosis. The aim of this retrospective study was to investigate the extent of differentiation in endometriosis-associated SMC (EMaSMC) in peritoneal endometriotic lesions. Methods We obtained biopsies from peritoneal endometriotic lesions (n = 60) and peritoneal sites distant from the endometriotic lesion (n = 60), as well as healthy peritoneum from patients without endometriosis (control tissue, n = 10). These controls were hysterectomy specimens from patients without endometriosis or adenomyos…

AdultendometriosisPathologymedicine.medical_specialtyBiopsyEndometriosisEstrogen receptorsmooth muscle metaplasiaPeritoneumRetrospective StudieProgesterone receptormedicinevasopressin receptorHumansMyocyteAdenomyosisEndometriosiOxytocin receptorRetrospective Studiesendometriosis; Oxytocin receptor; smooth muscle metaplasiaMyosin Heavy Chainsbusiness.industryRehabilitationMyosin Heavy ChainObstetrics and GynecologyCell DifferentiationMuscle SmoothMiddle Agedmusculoskeletal systemmedicine.diseaseSettore MED/40 - Ginecologia E OstetriciaImmunohistochemistrymedicine.anatomical_structureGene Expression RegulationPremenopauseReceptors EstrogenReproductive MedicineReceptors Oxytocinsmooth muscle actinImmunohistochemistryFemaleDesminPeritoneumReceptors ProgesteronebusinessHumanHuman Reproduction
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Imbalance between sympathetic and sensory innervation in peritoneal endometriosis

2011

To investigate possible mechanisms of pain pathophysiology in patients with peritoneal endometriosis, a clinical study on sensory and sympathetic nerve fibre sprouting in endometriosis was performed. Peritoneal lesions (n= 40) and healthy peritoneum (n= 12) were immunostained and analysed with anti-protein gene product 9.5 (PGP 9.5), anti-substance P (SP) and anti-tyrosine hydroxylase (TH), specific markers for intact nerve fibres, sensory nerve fibres and sympathetic nerve fibres, respectively, to identify the ratio of sympathetic and sensory nerve fibres. In addition, immune cell infiltrates in peritoneal endometriotic lesions were analysed and the nerve growth factor (NGF) and interleuki…

Sensory and sympathetic innervationPathologySympathetic nervous systemSympathetic Nervous SystemInterleukin-1betaEndometriosisSubstance PSubstance PSensory Receptor CellBehavioral Neurosciencechemistry.chemical_compoundNerve FibersGAP-43 ProteinGanglia SpinalNerve Growth FactorAscitic FluidMedicineEndometriosis; Neurotransmitters; Sensory and sympathetic innervationEndometriosiNGFImmunity CellularGanglia SympatheticNeurotransmittersMiddle AgedImmunohistochemistrymedicine.anatomical_structureNerve FiberIL-1βPain generation of endometriosis-related symptomFemaleUbiquitin ThiolesteraseHumanSensory nerveAdultmedicine.medical_specialtySensory Receptor CellsTyrosine 3-MonooxygenaseNeuriteImmunologyEndometriosisYoung AdultImmune cell infiltratePeritoneumInternal medicineHumansNerve Growth FactorsNeurotransmitterCell ProliferationInflammationEndocrine and Autonomic Systemsbusiness.industryStromal Cellmedicine.diseaseSensory Receptor CellsNerve growth factorEndocrinologychemistryLaparoscopyStromal CellsbusinessChronic pelvic painBrain, Behavior, and Immunity
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Neuroimmunomodulatory Alterations in Non-Lesional Peritoneum Close to Peritoneal Endometriosis

2012

<b><i>Objectives:</i></b> An imbalance in the ratio of sensory to sympathetic nerve fibre (NF) density in peritoneal endometriotic lesions (pEL) has recently been demonstrated and leads to the assumption that this preponderance of the sensory pro-inflammatory milieu is a major cause of pain in endometriosis. Therefore, the density of sensory and sympathetic NFs was determined in distal unaffected peritoneum of endometriosis patients to be able to detect possible alterations in unaffected peritoneum. <b><i>Methods:</i></b> In serial pEL sections (n = 40), lesional and matching unaffected peritoneum as well as healthy peritoneum (HP) from patien…

PathologyEndometriosisSympathetic nerveSubstance PSensory nerve fibreSubstance PPeritoneal Diseaseschemistry.chemical_compoundNerve growth factorPelvic painEndocrinologyhemic and lymphatic diseasesEndometriosiintegumentary systemvirus diseasesMiddle AgedInterleukin-1βImmunohistochemistrymedicine.anatomical_structureNeurologyImmunohistochemistryFemalemedicine.symptomPeritoneal diseasesPeritoneumHumanAdultmedicine.medical_specialtyAdolescentImmunologyEndometriosisEndocrine and Autonomic SystemYoung AdultPeritoneumAdrenergic FiberInternal medicinemedicineHumansPathogenesis endometriosiEndocrine and Autonomic Systemsbusiness.industryPelvic painmedicine.diseaseNerve growth factorEndocrinologychemistrySympathetic nerve fibrePeritoneal DiseasebusinessAdrenergic Fibers
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Eutopic endometrium from women with endometriosis does not exhibit neurotrophic properties

2012

The role of neurotrophins in eutopic endometrium from endometriosis-patients was investigated in a prospective study using immunofluorescence-staining, Western blot and a neuronal growth assay. The nerve growth factor is expressed in primary endometrial cell culture from women with and without endometriosis. Western blot analysis of endometrial biopsies or uterine fluid from patients with and without endometriosis shows no difference in the neurotrophin expression. We could not find a difference between patients with and without endometriosis with regards to the neurite outgrowth of sensory ganglia when treated with conditioned cultured medium or uterine fluid. This result refutes the assum…

AdultPathologymedicine.medical_specialtyNeuriteBlotting WesternImmunologyEndometriosisEndometriosisFluorescent Antibody TechniqueNeuriteEndometrial innervationAndrologyYoung AdultEndometrial innervation; Endometriosis; Pelvic painPelvic painWestern blotNeurotrophic propertieNerve Growth FactorNeuritesmedicineHumansImmunology and AllergyEutopic endometriumEndometriosiProspective cohort studyUterine Diseasesbiologymedicine.diagnostic_testbusiness.industryPelvic painDysmenorrhoeaMiddle Agedmedicine.diseaseSettore MED/40 - Ginecologia E OstetriciaNerve growth factorNeurologyCulture Media Conditionedbiology.proteinUterine DiseaseFemaleNeurology (clinical)medicine.symptombusinessNeurotrophinHuman
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High Lymph Vessel Density and Expression of Lymphatic Growth Factors in Peritoneal Endometriosis

2012

To investigate the occurrence of lymph vessels and lymphangiogenic growth factors in peritoneal lesions, we performed immunohistochemical staining of peritoneal lesions of 37 patients with antibodies against podoplanin (D2-40), lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1), prospero homeobox protein 1 (Prox-1), vascular epithelial growth factor (VEGF)-C/VEGF-D. Overall, 10 lesions were double stained against D2-40 and von Willebrand factor. The lymph vessel density in peritoneal lesion was significantly higher in comparison with healthy peritoneum. All lymph vessel makers could be detected, whereby the lymph vessel density of LYVE-1- and Prox-1-positive lymph vessels was signi…

Pathologychronic inflammatory proceMacrophageVascular Endothelial Growth Factor CVascular Endothelial Growth Factor DVesicular Transport ProteinsFluorescent Antibody TechniquePeritoneal DiseasesAntibodies Monoclonal Murine-Derivedlymphatic disseminationIntercellular Signaling Peptides and ProteinEndometriosiEndothelial CellObstetrics and GynecologyHomeodomain ProteinMiddle AgedImmunohistochemistryLymphangiogenesisLymphatic systemmedicine.anatomical_structureVascular endothelial growth factor CIntercellular Signaling Peptides and ProteinsFemaleLymphEndothelium LymphaticHumanAdultmedicine.medical_specialtyEndometriosisendometriosis; lymphatic dissemination; chronic inflammatory processlymphangiogenesiperitoneal endometriosiLymphatic VesselVesicular Transport ProteinPeritoneummedicineLymphatic vesselLymph node stromal cellHumansLymph sacsLymphatic VesselsHomeodomain ProteinsTumor Suppressor Proteinbusiness.industryMacrophagesTumor Suppressor ProteinsEndothelial CellsBiomarkerchronic inflammatory processPeritoneal DiseasebusinessBiomarkersReproductive Sciences
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Evidence of neurotrophic events due to peritoneal endometriotic lesions

2012

To investigate the neurotrophic properties of endometriosis, as well as the involvement of neurotrophic factors in the development of chronic pelvic pain in patients with endometriosis, we performed a prospective clinical study. The presence of neurotrophins was investigated in the peritoneal fluid (PF) of patients with peritoneal endometriotic lesions or adenomyosis, as well as from women with non-endometriotic adhesions and from women without endometriosis/adenomyosis/adhesions. The PF from patients with peritoneal endometriotic lesions was divided in three groups: asymptomatic endometriosis, minimal pain and severe pain. PF from patients with adenomyosis or with non-endometriotic adhesio…

AdultPathologymedicine.medical_specialtyImmunologyEndometriosisEndometriosisTissue AdhesionsBiochemistryCell LineYoung AdultPelvic painNeurotrophin 3Neurotrophic propertieNeurotrophic factorsGanglia SpinalmedicineNeuritesAnimalsAscitic FluidHumansImmunology and AllergyAdenomyosisProspective StudiesEndometriosiMolecular BiologyBrain-derived neurotrophic factorbiologybusiness.industryPeritoneal fluidPelvic painBrain-Derived Neurotrophic FactorMedicine (all)HematologyMiddle Agedmedicine.diseaseNerve growth factorPain generationbiology.proteinFemaleNeurotrophinmedicine.symptomChronic PainPeritoneumbusinessChickensAdenomyosisNeurotrophin
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Influence of nerve growth factor in endometriosis-associated symptoms

2011

To investigate the role of the nerve growth factor (NGF) in the development of dysmenorrhea/pelvic pain in patients with endometriosis, we performed a prospective, clinical, blind study. Peritoneal fluids (PFs) were obtained from patients with histologically proven endometriosis. Patients with endometriosis were divided into 7 different groups depending on their preoperative pain score and symptomatology: patients with no pain, patients with minimal pain (dysmenorrhea, pelvic pain, or both), and patients with severe pain (dysmenorrhea, pelvic pain, or both) and were used for the neuronal growth assay with cultured chicken dorsal root ganglia (DRG) and for Western blot analyses. Dorsal root …

endometriosisEndometriosisFluorescent Antibody TechniqueChick EmbryoGastroenterologypain generation of endometriosis-related symptomSeverity of Illness Indexendometriosis; pelvic pain; sensory nerve fibersGAP-43 ProteinGanglia SpinalGermanyNerve Growth FactorAscitic FluidProspective StudiesEndometriosiProspective cohort studyCells CulturedPain MeasurementNeuronsbiologymedicine.diagnostic_testObstetrics and Gynecologypelvic painMiddle AgedBlotAnesthesiaFemalemedicine.symptomNeurotrophinHumanAdultmedicine.medical_specialtyCalcitonin Gene-Related PeptideBlotting Westernsensory nerve fibersCalcitonin gene-related peptidesensory nerve fiberYoung AdultWestern blotDysmenorrheaInternal medicinemedicineAnimalsHumansbusiness.industryAnimalPelvic painNeuronmedicine.diseaseSettore MED/40 - Ginecologia E OstetriciaProspective StudieNerve growth factorbiology.proteinbusiness
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Overexpression of nerve growth factor in peritoneal fluid from women with endometriosis may promote neurite outgrowth in endometriotic lesions

2011

To investigate the role of the nerve growth factor (NGF) in the endometriosis-associated innervation in the development of endometriosis- associated symptoms, 41 peritoneal fluid samples (PF) from patients with surgically and histologically proven endometriosis and 20 PF from patients with other gynecologic conditions were analyzed with Western blot and a novel in vitro model using dorsal root ganglia (DRG) to show neuronal outgrowth; endometrial cells also were analyzed. The results suggest that the PF of endometriosis patients and endometriotic lesions have neurotropic properties, because the Western blot analysis and the cell culture stainings showed NGF expression, and the neurite outgr…

medicine.medical_specialtyPathologySensory Receptor CellsNeuriteBlotting WesternEndometriosisEndometriosisFluorescent Antibody TechniqueNerve fiberNeuriteChick Embryosensory nerve fiberPelvic PainEndometriumSensory Receptor Cellsensory nerveEndometriumpain conductionDysmenorrheaWestern blotGanglia SpinalNerve Growth FactorNeuritesmedicineAnimalsAscitic FluidHumansEndometriosiCells Culturedpathophysiology of endometriosis-related painGynecologymedicine.diagnostic_testAnimalbusiness.industryPeritoneal fluidObstetrics and Gynecologymedicine.diseaseEndometriosis; pain conduction; sensory nervemedicine.anatomical_structureNerve growth factornervous systemReproductive MedicineFemalebusinessHumanSensory nerveFertility and Sterility
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