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RESEARCH PRODUCT

Imbalance between sympathetic and sensory innervation in peritoneal endometriosis

Sylvia MechsnerVito ChianteraMaria Luisa Barcena De ArellanoAchim SchneiderGiuseppe Filiberto VercellinoJulia ArnoldCarola Rüster

subject

Sensory and sympathetic innervationPathologySympathetic nervous systemSympathetic Nervous SystemInterleukin-1betaEndometriosisSubstance PSubstance PSensory Receptor CellBehavioral Neurosciencechemistry.chemical_compoundNerve FibersGAP-43 ProteinGanglia SpinalNerve Growth FactorAscitic FluidMedicineEndometriosis; Neurotransmitters; Sensory and sympathetic innervationEndometriosiNGFImmunity CellularGanglia SympatheticNeurotransmittersMiddle AgedImmunohistochemistrymedicine.anatomical_structureNerve FiberIL-1βPain generation of endometriosis-related symptomFemaleUbiquitin ThiolesteraseHumanSensory nerveAdultmedicine.medical_specialtySensory Receptor CellsTyrosine 3-MonooxygenaseNeuriteImmunologyEndometriosisYoung AdultImmune cell infiltratePeritoneumInternal medicineHumansNerve Growth FactorsNeurotransmitterCell ProliferationInflammationEndocrine and Autonomic Systemsbusiness.industryStromal Cellmedicine.diseaseSensory Receptor CellsNerve growth factorEndocrinologychemistryLaparoscopyStromal CellsbusinessChronic pelvic pain

description

To investigate possible mechanisms of pain pathophysiology in patients with peritoneal endometriosis, a clinical study on sensory and sympathetic nerve fibre sprouting in endometriosis was performed. Peritoneal lesions (n= 40) and healthy peritoneum (n= 12) were immunostained and analysed with anti-protein gene product 9.5 (PGP 9.5), anti-substance P (SP) and anti-tyrosine hydroxylase (TH), specific markers for intact nerve fibres, sensory nerve fibres and sympathetic nerve fibres, respectively, to identify the ratio of sympathetic and sensory nerve fibres. In addition, immune cell infiltrates in peritoneal endometriotic lesions were analysed and the nerve growth factor (NGF) and interleukin (IL)-1β expression was correlate with the nerve fibre density. Peritoneal fluids from patients with endometriosis (n= 40) and without endometriosis (n= 20) were used for the in vitro neuronal growth assay. Cultured chicken dorsal root ganglia (DRG) and sympathetic ganglia were stained with anti-growth associated protein 43 (anti-GAP 43), anti-SP and anti-TH. We could detect an increased sensory and decreased sympathetic nerve fibres density in peritoneal lesions compared to healthy peritoneum. Peritoneal fluids of patients with endometriosis compared to patients without endometriosis induced an increased sprouting of sensory neurites from DRG and decreased neurite outgrowth from sympathetic ganglia. In conclusion, this study demonstrates an imbalance between sympathetic and sensory nerve fibres in peritoneal endometriosis, as well as an altered modulation of peritoneal fluids from patients with endometriosis on sympathetic and sensory innervation which might directly be involved in the maintenance of inflammation and pain. © 2011 Elsevier Inc.

yearjournalcountryeditionlanguage
2011-05-31Brain, Behavior, and Immunity
https://doi.org/10.1016/j.bbi.2011.08.004