6533b861fe1ef96bd12c4e5b

RESEARCH PRODUCT

Overexpression of nerve growth factor in peritoneal fluid from women with endometriosis may promote neurite outgrowth in endometriotic lesions

Sylvia MechsnerJulia ArnoldVito ChianteraAchim SchneiderFiliberto VercellinoMaria Luisa Barcena De Arellano

subject

medicine.medical_specialtyPathologySensory Receptor CellsNeuriteBlotting WesternEndometriosisEndometriosisFluorescent Antibody TechniqueNerve fiberNeuriteChick Embryosensory nerve fiberPelvic PainEndometriumSensory Receptor Cellsensory nerveEndometriumpain conductionDysmenorrheaWestern blotGanglia SpinalNerve Growth FactorNeuritesmedicineAnimalsAscitic FluidHumansEndometriosiCells Culturedpathophysiology of endometriosis-related painGynecologymedicine.diagnostic_testAnimalbusiness.industryPeritoneal fluidObstetrics and Gynecologymedicine.diseaseEndometriosis; pain conduction; sensory nervemedicine.anatomical_structureNerve growth factornervous systemReproductive MedicineFemalebusinessHumanSensory nerve

description

To investigate the role of the nerve growth factor (NGF) in the endometriosis-associated innervation in the development of endometriosis- associated symptoms, 41 peritoneal fluid samples (PF) from patients with surgically and histologically proven endometriosis and 20 PF from patients with other gynecologic conditions were analyzed with Western blot and a novel in vitro model using dorsal root ganglia (DRG) to show neuronal outgrowth; endometrial cells also were analyzed. The results suggest that the PF of endometriosis patients and endometriotic lesions have neurotropic properties, because the Western blot analysis and the cell culture stainings showed NGF expression, and the neurite outgrowth of DRG treated with PF of patients with endometriosis was significantly higher than when treated with PF of patients without endometriosis. Furthermore, blocking NGF with both anti-NGF and K252a leads to a significant decrease in neurite outgrowth. ©2011 by American Society for Reproductive Medicine.

yearjournalcountryeditionlanguage
2011-01-01Fertility and Sterility
https://doi.org/10.1016/j.fertnstert.2010.10.023