6533b822fe1ef96bd127cc76

RESEARCH PRODUCT

Eutopic endometrium from women with endometriosis does not exhibit neurotrophic properties

Maria Luisa Barcena De ArellanoGiuseppe Filiberto VercellinoMatthias BlöchleJulia ArnoldJulia ArnoldMagdalena StaubeJ. BartleyVito ChianteraSylvia MechsnerFrank SacherAchim Schneider

subject

AdultPathologymedicine.medical_specialtyNeuriteBlotting WesternImmunologyEndometriosisEndometriosisFluorescent Antibody TechniqueNeuriteEndometrial innervationAndrologyYoung AdultEndometrial innervation; Endometriosis; Pelvic painPelvic painWestern blotNeurotrophic propertieNerve Growth FactorNeuritesmedicineHumansImmunology and AllergyEutopic endometriumEndometriosiProspective cohort studyUterine Diseasesbiologymedicine.diagnostic_testbusiness.industryPelvic painDysmenorrhoeaMiddle Agedmedicine.diseaseSettore MED/40 - Ginecologia E OstetriciaNerve growth factorNeurologyCulture Media Conditionedbiology.proteinUterine DiseaseFemaleNeurology (clinical)medicine.symptombusinessNeurotrophinHuman

description

The role of neurotrophins in eutopic endometrium from endometriosis-patients was investigated in a prospective study using immunofluorescence-staining, Western blot and a neuronal growth assay. The nerve growth factor is expressed in primary endometrial cell culture from women with and without endometriosis. Western blot analysis of endometrial biopsies or uterine fluid from patients with and without endometriosis shows no difference in the neurotrophin expression. We could not find a difference between patients with and without endometriosis with regards to the neurite outgrowth of sensory ganglia when treated with conditioned cultured medium or uterine fluid. This result refutes the assumed neurotrophic properties of eutopic endometrium of patients with endometriosis. © 2012 Elsevier B.V.

yearjournalcountryeditionlanguage
2012-01-01
10.1016/j.jneuroim.2012.04.020http://hdl.handle.net/11695/5538