Search results for "Forensic"

Differential specificity of substrate-attached lectins stimulating spreading of GH3-cells under serum-free, hormone-supplemented culture conditions

1982

Most mammalian cells are capable of growth in culture only when they are supplied with an appropriate substrate to which they can adhere and spread. To prepare suitable substrates different lectins were attached onto polystyrene tissue-culture dishes after coating with polylysine. GH3-cells (a pituitary-tumor-cell line) were seeded into the culture dishes containing serum-free, hormone-supplemented medium. When succinylated Concanavalin A (s-Con A), which binds specifically to mannose residues, is attached to the surface an extraordinary spreading of GH3-cells is induced within 15 to 20 min after seeding. Other lectins with a different sugar-binding specificity are less effective in inducin…

Electron microscopic and histochemical studies on the function of the branchial heart appendage (pericardial gland) in Sepia officinalis (l.)

1971

Die elektronenmikroskopischen Untersuchungen am Kiemenherzanhang von Sepia officinalis zeigen, das diesem Organ wie den Venenanhangen exkretorische Funktionen zukommen. Die Pericardialdruse erweist sich als ein Derivat des Kiemenherzens. Ihre Hauptmasse besteht aus einem polar organisierten Faltenepithel, dessen Ultrastrukturmerkmale (basaler Faltenapparat, Gehalt an Mitochondrien und Lysosomen, sekretorisch aktiver Mikrovillisaum) anderen Exkretionsorganen entsprechend auf aktive Ionentransportvorgange hindeuten. Es ist dem auseren Kiemenherzepithel homolog und kommt in ahnlicher Ausbildung auch in anderen Organen (Zentralherz, Kieme) vor. Die polygonalen Zellen der Randzone sind dem zentr…

Elektronenmikroskopische und histochemische Untersuchungen zur Funktion der Branchialdr�se (Parabranchialdr�se) der Cephalopoda

1973

Licht- und elektronenmikroskopische Untersuchungen an der Branchialdruse verschiedener Cephalopoden (Sepia officinalis, Octopus vulgaris, Loligo vulgaris, Eledone moschata) zeigen, das das Drusengewebe des stark vaskularisierten Organs aus einem sekretorisch aktiven Zelltypus mit ausgepragtem endoplasmatischen Retikulum besteht. In den Zisternen des endoplasmatischen Retikulums lassen sich granulare und ringformige Strukturen (Durchmesser: 45–65 A und 170 A) nachweisen, die in Grose und Gestalt mit den Hamocyanin-Einheiten in den Gefasen und Lakunen ubereinstimmen und durch randlichen Zerfall der Zellen in den Blutraum auszuwandern scheinen. Die histochemischen Untersuchungen ergeben eine p…

Elektronenmikroskopische Studien �ber eine extrazellul�r auftretende Substanz in der Muskulatur des Insekts Oncopeltus fasciatus Dallas

1973

Im Stroma von Aorta und Retrocerebralkomplex sowie von verschiedenen Muskeln (Oberschenkel-, Mitteldarm-, Speicheldrusen-, Samenleiter- und Eileitermuskeln), jedoch nicht im Stroma der Flugmuskulatur und des Herzschlauches, kommt eine fibrillar strukturierte Substanz (X-Substanz) von auserordentlich hoher Elektronendichte vor.

�ber den Feinbau der Schwimmblase von Anguilla vulgaris L.

1961

Three-dimensional image analytical detection of intussusceptive pillars in murine lung

2015

A variety of diseases can lead to loss of lung tissue. Currently, this can be treated only symptomatically. In mice, a complete compensatory lung growth within 21 days after resection of the left lung can be observed. Understanding and transferring this concept of compensatory lung growth to humans would greatly improve therapeutic options. Lung growth is always accompanied by a process called angiogenesis forming new capillary blood vessels from preexisting ones. Among the processes during lung growth, the formation of transluminal tissue pillars within the capillary vessels (intussusceptive pillars) is observed. Therefore, pillars can be understood as an indicator for active angiogenesis …

Effects of caspase inhibitors (z-VAD-fmk, z-VDVAD-fmk) on Nile Red fluorescence pattern in 7-ketocholesterol-treated cells: Investigation by flow cyt…

2007

Background: The 7-ketocholesterol (7KC)-induced cell death has some characteristics of apoptosis and is associated with polar lipid accumulation. So, we investigated the effects of the broad-spectrum caspase inhibitor z-VAD-fmk and of the caspase-2 inhibitor z-VDVAD-fmk on lipid profile evaluated by staining with Nile Red (NR). Methods: The 7KC-treated human monocytic U937 cells were cultured in the absence or in the presence of the caspase inhibitors z-VAD-fmk or z-VDVAD-fmk. When staining with NR is performed, neutral and polar lipids have yellow and orange/red emission, respectively, and fluorescence was then analyzed by flow cytometry (FCM) and by confocal laser scanning microscopy (CLS…

Origin of metazoan adhesion molecules and adhesion receptors as deduced from cDNA analyses in the marine sponge Geodia cydonium: a review.

1997

The phylogenetic relationships of the kingdom Animalia (Metazoa) have long been questioned. Whether the lowest eukaryotic multicellular organisms, the metazoan phylum Porifera (sponges), independently evolved multicellularity from a separate protist lineage (polyphyly of animals) or whether they were derived from the same protist group as the other animal phyla (monophyly) remains unclear. Analyses of the genes that are typical for multicellularity, e.g. those coding for adhesion molecules (galectin) and adhesion receptors (receptor tyrosine kinase, integrin receptor, receptors featuring scavenger receptor cysteine-rich domains) or elements involved in signal transduction pathways (G-protei…

Evolution of tissue-specific keratins as deduced from novel cDNA sequences of the lungfish Protopterus aethiopicus.

2005

Lungfishes are possibly the closest extant relatives of the land vertebrates (tetrapods). We report here the cDNA and predicted amino acid sequences of 13 different keratins (ten type I and three type II) of the lungfish Protopterus aethiopicus. These keratins include the orthologs of human K8 and K18. The lungfish keratins were also identified in tissue extracts using two-dimensional polyacrylamide gel electrophoresis, keratin blot binding assays and immunoblotting. The identified keratin spots were analyzed by peptide mass fingerprinting which assigned seven sequences (inclusively Protopterus K8 and K18) to their respective protein spot. The peptide mass fingerprints also revealed the fac…

Tracing keratin evolution: catalog, expression patterns and primary structure of shark (Scyliorhinus stellaris) keratins.

1998

We have studied individual keratins of an elasmobranch, the shark Scyliorhinus stellaris (the lesser-spotted dogfish). From various shark tissues, notably skin and stomach, cytoskeletal proteins were isolated and then separated by two-dimensional polyacrylamide gel electrophoresis. Using complementary keratin blot-binding assays and immunoblotting, among these proteins we identified a variety of type I and type II keratins. According to their tissue-specific expression, we distinguished Is and IIs keratins from IE and IIE keratins ("S" and "E" from "simple epithelial" and "epidermal", respectively). Guinea pig antibodies which in immunoblots specifically labeled the entire range of identifi…