Search results for "Forming."
showing 10 items of 1704 documents
Human CD4+CD25+ T cells derived from the majority of atopic donors are able to suppress TH1 and TH2 cytokine production
2003
Abstract Background: Recently, it has been established that CD4 + CD25 + T cells with regulatory capacity are present in human peripheral blood, inhibiting allogeneic proliferation and cytokine production of preactivated CD4 + CD25 − respond-er T cells. Objective: The aim of this study was to analyze in an allergen-specific setting whether such regulatory CD4 + CD25 + T cells also exist and function normally in atopic individuals, especially concerning the inhibition of T H 2 cytokines. Methods: For this purpose, CD4 + CD25 − or CD4 + CD25 + T cells from donors allergic to grass or birch pollen (mainly with rhinitis) or from healthy nonatopic donors were stimulated in the presence of autolo…
MicroRNAs play a central role in molecular dysfunctions linking inflammation with cancer.
2013
It is now largely admitted that a pro-inflammatory environment may curtail anti-tumor immunity and favor cancer initiation and progression. The discovery that small non-coding regulatory RNAs, namely microRNAs (miRNAs), regulate all aspects of cell proliferation, differentiation, and function has shed a new light on regulatory mechanisms linking inflammation and cancer. Thus, miRNAs such as miR-21, miR-125b, miR-155, miR-196, and miR-210 that are critical for the immune response or hypoxia are often overexpressed in cancers and leukemias. Given the high number of their target transcripts, their deregulation may have a number of deleterious consequences, depending on the cellular context. In…
Inhibition of human allergic T-helper type 2 immune responses by induced regulatory T cells requires the combination of interleukin-10-treated dendri…
2006
Summary Background In grass pollen-allergic individuals, T cell anergy can be induced by IL-10-treated dendritic cells (IL-10-DC) resulting in the suppression of T helper type 1 (Th1) as well as Th2 cells. This study was performed to analyse whether such IL-10-DC-treated T cells are able to act as regulatory T cells (Treg) suppressing the function of other T cells in the periphery. As transforming growth factor (TGF)-β is also a potential inducer of Treg, we additionally analysed the inhibitory capacity of TGF-β-treated T cells in this system. Materials and Methods Freshly isolated CD4+ or CD4+CD25− T cells from grass pollen-allergic donors were stimulated with autologous mature monocyte-de…
Reciprocal IFN-gamma and TGF-beta responses regulate the occurrence of mucosal inflammation.
1997
The above new findings concerning the immunological mechanisms governing mucosa, immune responses and oral tolerance in TCR-transgenic mice, as well as those operative in mice with experimental colitis, greatly expand our understanding of the processes that normally control mucosal inflammation and possibly other types of inflammation as well (Fig. 1). They indicate that, in the nondiseased mouse, ingested proteins evoke a Th1-cell (IFN-gamma) response in the mucosal follicles that is quickly counter-regulated by induction of T-cell anergy/deletion, if this Th1-cell response is inhibited (experimentally by anti-IL-12), TGF beta-producing cells appear, and these are capable of active immune …
Play together, think alike: Shared mental models in expert music improvisers
2015
International audience; When musicians improvise together, they tend to agree beforehand on a common structure (e.g. a jazz standard) which helps them coordinate. However, in the particular case of collective free improvisation (CFI), musicians deliberately avoid having such a referent. How, then, can they coordinate? We propose that CFI musicians who have experience playing together come to share higher-level knowledge, which is not piece-specific but rather task-specific: an implicit mental model of what it is to improvise freely. We tested this hypothesis on a group of 19 expert improvisers from the Parisian CFI community, who had various degrees of experience playing with one another. D…
Physico-chemical and mechanical characterization of in-situ forming xyloglucan gels incorporating a growth factor to promote cartilage reconstruction
2016
Abstract The development of growth factors is very promising in the field of tissue regeneration but specifically designed formulations have to be developed in order to enable such new biological entities (NBEs). In particular, the range of therapeutic concentrations is usually very low compared to other active proteins and the confinement in the target site can be of crucial importance. In-situ forming scaffolds are very promising solutions for minimally invasive intervention in cartilage reconstruction and targeting of NBEs. In this work injectable, in-situ forming gels of a temperature responsive partially degalactosylated xyloglucan (Deg-XG) incorporating the growth factor FGF-18 are fo…
In vitro evaluation of poloxamer in situ forming gels for bedaquiline fumarate salt and pharmacokinetics following intramuscular injection in rats
2019
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Experimental calibration of flat jacks for in-situ testing of masonry
2018
Flat-jack testing method is one of the most commonly used techniques for the structural assessment of existing masonry structures. Single and double flat jacks are usually adopted to evaluate the acting normal stress, or the compressive behaviour of masonry material. Test procedures are codified by international standards (e.g., A.S.T.M D4729-87; C1196-04; C1197-04, R.I.L.E.M TC 177–MDT D.4; R.I.L.E.M. TC 177–MDT D.5), which provide the preliminary calibration of an experimental coefficient (km) and of the effective area (Aeff), which determination influences significantly the reliability of the test. This article presents the result of an experimental study on the calibration of flat jacks…
Total RNA-isolation of abdominal hernia of rats for quantitative real-time reverse transcription (RT) PCR assays.
2007
Abstract Increasing complications in incisional hernia surgery call for novel treatments. A gene expression analysis of injured tissues displays important parameters for tissue regeneration. Until today, no reliable method has been described for a quantitative gene expression analysis of hernia tissues. In this work, a protocol is described for the isolation of DNA‐free total RNA of incisional hernias for the first time. Moreover, real‐time RT PCR assays for collagen type I and III and TGF‐β1 are demonstrated for relative gene expression analyses. Both methods enable relative gene expression analyses of hernia tissues for the first time.
Energy efficiency analysis in Incremental Sheet Forming operations
2012
In literature, manufacturing processes have been studied with different approaches, but a lack in terms of their environmental impact and energy required is still present. The aim of this paper is to compute some energy efficiency analysis on a specific manufacturing process. More in detail, the attention will be focused on the Incremental Sheet Forming process in order to evaluate the required energy and how it changes for changing process conditions. Accordingly, a wide experimental investigation has been executed taking into account different process parameters (i.e. tool depth step, punch diameter, wall inclination angle and sheet thickness). The total energy consumption has been monito…