Search results for "Frame"
showing 10 items of 1767 documents
Direct Identification of Each Specific Mutation in Codon 12 and 13 of ci-ki-ras2 by SSCP Analysis
1998
We compared the SSCP behaviour of the DNA fragments containing c-ki-ras 2 wild type 12 and 13 codons or each of the 12 possible point mutated sequences in these two codons. We found that a single electrophoresis condition was sufficient to distinguish each specific mutation from the other 11 and from the wild type sequence. This observation makes it possible to identify each specific mutation directly by SSCP without any need for reamplification and sequencing.
The ATC1 gene encodes a cell wall-linked acid trehalase required for growth on trehalose in Candida albicans.
2004
After screening a Candida albicans genome data base, the product of an open reading frame (IPF 19760/CA2574) with 41% identity to Saccharomyces cerevisiae vacuolar acid trehalase (Ath1p) was identified and named Atc1p. The deduced amino acid sequence shows that Atc1p contains an N-terminal hydrophobic signal peptide and 20 potential sites for N-glycosylation. C. albicans homozygous mutants that lack acid trehalase activity were constructed by gene disruption at the two ATC chromosomal alleles. Analysis of these null mutants shows that Atc1p is localized in the cell wall and is required for growth on trehalose as a carbon source. An Atc1p endowed with acid trehalase activity was obtained by …
A DNA region ofTorulaspora delbrueckii containing theHIS3 gene: sequence, gene order and evolution
2003
We cloned a genomic DNA fragment of the yeast Torulaspora delbrueckii by complementation of a Saccharomyces cerevisiae his3 mutant strain. DNA sequence analysis revealed that the fragment contained two complete ORFs, which share a high similarity with S. cerevisiae His3p and Mrp51p, respectively. The cloned TdHIS3 gene fully complemented the his3 mutation of S. cerevisiae, confirming that it encodes for the imidazoleglycerol-phosphate dehydrate of T. delbrueckii. Two additional ORFs, with a high homology to S. cerevisiae PET56 and DED1 genes, were mapped upstream and downstream from TdHIS3 and TdMRP51, respectively. This genetic organization is analogous to that previously found in Saccharo…
A genomic study of the inter-ORF distances in Saccharomyces cerevisiae.
2006
The genome of eukaryotic microbes is usually quite compacted. The yeast Saccharomyces cerevisiae is one of the best-known examples. Open reading frames (ORFs) occupy about 75% of the total DNA sequence. The existence of other, non-protein coding genes and other genetic elements leaves very little space for gene promoters and terminators. We have performed an in silico study of inter-ORF distances that shows that there is a minimum distance between two adjacent ORFs that depends on the relative orientation between them. Our analyses suggest that different kinds of promoters and terminators exist with regard to their length and ability to overlap each other. The experimental testing of some p…
Registration of arbitrary multi-view 3D acquisitions
2013
International audience; To register 3D meshes representing smooth surfaces we track the 3D digitization system using photogrammetric techniques and calibrations. We present an example by digitizing a 800 mm x 600 mm portion of a car door. To increase the tracking accuracy the 3D scanner is placed in a cubic frame of side 0.5 m covered with 78 targets. The target frame moves in a volume that is approximately 1100 mm x 850 mm x 900 mm, to digitize the area of interest. Using four cameras this target frame is tracked with of an accuracy of 0.03 mm spatially and 0.180 mrad angularly. A registration accuracy between 0.1 mm and 2 mm is reached. This method can be used for the registration of mesh…
FLEX End-to-End Mission Performance Simulator
2016
The FLuorescence EXplorer (FLEX) mission, selected as the European Space Agency's eighth Earth Explorer, aims to globally measure the sun-induced-chlorophyll-fluorescence spectral emission from terrestrial vegetation. In the frame of the FLEX mission, several industrial and scientific studies have analyzed the instrument design, image processing algorithms, or modeling aspects. At the same time, a common tool is needed to address the overall FLEX mission performance by combining all these features. For this reason, an end-to-end mission performance simulator has been developed for the FLEX mission (FLEX-E). This paper describes the FLEX-E software design, which combines the generation of co…
A General Frame for Building Optimal Multiple SVM Kernels
2012
The aim of this paper is to define a general frame for building optimal multiple SVM kernels. Our scheme follows 5 steps: formal representation of the multiple kernels, structural representation, choice of genetic algorithm, SVM algorithm, and model evaluation. The computation of the optimal parameter values of SVM kernels is performed using an evolutionary method based on the SVM algorithm for evaluation of the quality of chromosomes. After the multiple kernel is found by the genetic algorithm we apply cross validation method for estimating the performance of our predictive model. We implemented and compared many hybrid methods derived from this scheme. Improved co-mutation operators are u…
Priority-based initial access for URLLC traffic in massive IoT networks: Schemes and performance analysis
2020
Abstract At a density of one million devices per square kilometer, the10’s of billions of devices, objects, and machines that form a massive Internet of things (mIoT) require ubiquitous connectivity. Among a massive number of IoT devices, a portion of them require ultra-reliable low latency communication (URLLC) provided via fifth generation (5G) networks, bringing many new challenges due to the stringent service requirements. Albeit a surge of research efforts on URLLC and mIoT, access mechanisms which include both URLLC and massive machine type communications (mMTC) have not yet been investigated in-depth. In this paper, we propose three novel schemes to facilitate priority-based initial …
Two Times Four Integrative Levels of Analysis: A Framework
2001
Walsham (2000) urged Information Systems researchers to truly cover all levels of analysis from the individual to the societal in their research agenda. In this paper, we accept his challenge by proposing a research framework offour integrative levels of analysis: individual, group/activity, organizational, and societal. Each level is divided into two viewpoints: intra (single case) and inter (multiple cases, relational, comparative). In addition, the temporal/historical dimension should be applied on all levels and viewpoints. We argue that although all of the levels and viewpoints of the “2 × 4 + history” framework cannot be thoroughly covered in any single study, researchers should alway…
The Architecture of a Complex System: The Oradea Metropolitan Area in Romania
2017
This chapter approaches the experience of inter-municipal cooperation in the metropolitan area corresponding to one of Romania’s largest cities. It shows that, in a national context not yet ready for the notion of metropolitan authority, making metropolitan cooperation work and achieving palpable results in terms of economies of scale and scope in service delivery require the setting up of a very complex structure. This complexity poses challenges in terms of decision-making and inter-sectoral coordination, while at the same time creates favourable premises for the political accountability of inter-municipal cooperation arrangements. This complexity has also proven to be the seed for volunt…