Search results for "GATES"
showing 10 items of 176 documents
Synthesis and Structure-Activity Relationships of Amino Acid Conjugates of Cholanic Acid as Antagonists of the EphA2 Receptor
2013
The Eph–ephrin system plays a critical role in tumor growth and vascular functions during carcinogenesis. We had previously identified cholanic acid as a competitive and reversible EphA2 antagonist able to disrupt EphA2-ephrinA1 interaction and to inhibit EphA2 activation in prostate cancer cells. Herein, we report the synthesis and biological evaluation of a set of cholanic acid derivatives obtained by conjugation of its carboxyl group with a panel of naturally occurring amino acids with the aim to improve EphA2 receptor inhibition. Structure-activity relationships indicate that conjugation of cholanic acid with linear amino acids of small size leads to effective EphA2 antagonists whereas …
The dark side of foetal bovine serum in extracellular vesicle studies
2022
Extracellular vesicles (EVs) have been shown to be involved in cell-cell communication and to take part in both physiological and pathological processes. Thanks to their exclusive cargo, which includes proteins, lipids, and nucleic acids from the originating cells, they are gaining interest as potential biomarkers of disease. In recent years, their appealing features have been fascinating researchers from all over the world, thus increasing the number of in vitro studies focused on EV release, content, and biological activities. Cultured cell lines are the most-used source of EVs; however, the EVs released in cell cultures are influenced by the cell culture conditions, such as the use of fo…
Probing ensemble polymorphism and single aggregate structural heterogeneity in insulin amyloid self-assembly.
2020
Ensembles of protein aggregates are characterized by a nano- and micro-scale heterogeneity of the species. This diversity translates into a variety of effects that protein aggregates may have in biological systems, both in connection to neurodegenerative diseases and immunogenic risk of protein drug products. Moreover, this naturally occurring variety offers unique opportunities in the field of protein-based biomaterials. In the above-mentioned fields, the isolation and structural analysis of the different amyloid types within the same ensemble remain a priority, still representing a significant experimental challenge. Here we address such complexity in the case of insulin for its relevance…
Glycosylated macromolecular conjugates of antiviral drugs with a polyaspartamide.
2004
Two new polymeric conjugates for specific liver targeting were prepared by conjugation of sugar moieties and antiviral drugs to alpha, beta-poly[N-2-(hydroxyethyl)-DL-aspartamide] (PHEA). PHEA-galactopyranosylphenylthiocarbamide-mono-O-succinylganciclovir (conjugate 7) and PHEA-mannopyranosylphenylthiocarbamide-O-succinylacyclovir (conjugate 8) were synthesized according to a multi-step procedure which allowed for obtaining high product yield and process standardization. Conjugate 7 contained 7.5 and 8.5% of galactose and ganciclovir (substituent/repeating unit, mol/mol), respectively, and conjugate 8 contained 14.2 and 10.8% of mannose and acyclovir, respectively. In vitro studies demonstr…
Interference Cancellation for LoRa Gateways and Impact on Network Capacity
2021
In this paper we propose LoRaSyNc (LoRa receiver with SyNchronization and Cancellation), a second generation LoRa receiver that implements Successive Interference Cancellation (SIC) and time synchronization to improve the performance of LoRa gateways. Indeed, the chirp spread spectrum modulation employed in LoRa experiences very high capture probability, and cancelling the strongest signal in case of collisions can significantly improve the cell capacity. An important feature of LoRaSyNc is the ability to track the frequency and clock drifts between the transmitter and receiver, during the whole demodulation of the interfered frame. Due to the use of low-cost oscillators on end-devices, a s…
Land Snail Fauna of “Porţile de Fier” (“Iron Gates”) Nature Park (Banat, Romania)
2014
Abstract The paper presents an analysis of terrestrial mollusc fauna of the “Iron Gates” Nature Park. Various types of habitats (e.g. forests, rocks, riparian areas) and substrates (e.g. limestone, conglomerate, crystalline schist) were analyzed. A total of 45 species of terrestrial gastropods were identified in 17 sampling points, four of which are mentioned for the first time in the area. Six other species cited in the literature were not found. Limestone substrate allows the development of large populations of terrestrial gastropods, but the specific diversity is larger when it is associated with a forest habitat. The current legal and illegal exploitation of limestone threatens the moll…
Physical and mechanical properties of sustainable hydraulic mortar based on marble slurry with waste glass
2021
This paper aims to propose and characterize a sustainable hydraulic mortar entirely obtained by the reuse of waste materials, with marble slurry coming from quarries in the north-western Sicily and glass powder coming from a waste collection plant in Marsala (Province of Trapani). The first was used as raw material to produce the mortar binder by a kilning and slaking process, while the second was used as a pozzolanic additive. The chemical and morphological characterization of the marble slurry was done by XRD, FTIR, STA and SEM analyses. Glass powder was analyzed through particle size distribution measurements, XRD and standard pozzolanic tests. Hydraulic mortars constituted by slaked lim…
Distribution patterns of neoglycoprotein-binding sites (endogenous lectins) and lectin-reactive glycoconjugates during cartilage and bone formation i…
1995
The distribution of endogenous lectins, visualized by labelled neoglycoproteins, and of defined oligosaccharide structures, reactive with plant lectins, during fetal development of the fingers was analyzed in sections of human 3- to 8-month-old fetal specimens. Chondrogenesis as well as ossification were correlated with characteristic modulations in the expression of both glycoligand-binding molecules and characteristic carbohydrate structures. Occurrence of xylose-specific receptors was judged to be an early sign of cartilage development. Similarly, α-mannosyl residues that had been attached to labelled carrier proteins were strongly bound by the extracellular matrix already during early s…
Tetrathiafulvalene-Capped Hybrid Materials for the Optical Detection of Explosives
2013
[EN] Mesoporous silica microparticles capped with TTF moieties and containing a ruthenium dye in the pores were used for the turn-on optical detection of the nitroaromatic explosives Tetryl and TNT via a selective pore uncapping and release of the entrapped dye.
Enzyme-Mediated Controlled Release Systems by Anchoring Peptide Sequences on Mesoporous Silica Supports
2011
[EN] Gated community: Peptides anchored to the surface of silica mesoporous supports by a valid procedure act as gatekeepers. In this way, "zero release" supports that selectively deliver the cargo in the presence of a suitable peptidase are obtained (see picture, red spheres: cargo, colored chains: peptides). © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.