Search results for "GREEN"
showing 10 items of 1660 documents
The role of greenways in the sustainable mobility development: The study case of western municipalities of Sicily
2018
As is known, mobility policies based on greenways represent an effective and comprehensive response to the demand for travel and recreation and to environmental protection as well. There are several reasons why spreading cycling mobility contributes to making cities more liveable: a more efficient urban transport system, less congested roads and, consequently, less noisy roads, more security on the roads, etc. Furthermore, cycling helps fight climate change and the conservation of fossil fuels, promotes forms of sustainable tourism development and, not least, physical activity and, thus, the health of citizens. Whereas greenways may constitute a strategic opportunity for the promotion of la…
Reshaping the sea-land interface through sustainable mobility: a project for a greenway in western Sicily
2018
This work illustrates the planning experience, still underway, carried out by five municipalities of western Sicily (Italy) in the preparation of a sustainable urban development strategy in the framework of the EU’s urban agenda 2014-2020. The strategy, whose general objectives are strengthening territorial cohesion and increasing accessibility to local resources, focuses on sustainable mobility corridors in coastal areas as the instruments through which such objectives can be better achieved and reciprocally integrated. After an exploration of the literature, the article provides an analysis of the potential demand for sustainable mobility in the given urban system, an explanation of the c…
Generation of reporter plasmids containing defined base modifications in the DNA strand of choice
2012
Physiological effects of DNA bases other than A, G, C, and T as well as ways of removal of such bases from genomes are studied intensely. Methods for targeted insertion of modified bases into DNA, therefore, are highly demanded in the fields of DNA repair and epigenetics. This article describes efficient procedures for incorporation of modified DNA bases into a plasmid-borne enhanced green fluorescent protein (EGFP) gene. The procedure exploits excision of a stretch of 18 nt from either the transcribed or nontranscribed DNA strand with the help of the sequence-specific nicking endonucleases Nb.Bpu10I and Nt.Bpu10I. The excised single-stranded oligonucleotide is then swapped for a synthetic …
Gene silencing induced by oxidative DNA base damage: association with local decrease of histone H4 acetylation in the promoter region
2010
Oxidized DNA bases, particularly 7,8-dihydro-8-oxoguanine (8-oxoG), are endogenously generated in cells, being a cause of carcinogenic mutations and possibly interfering with gene expression. We found that expression of an oxidatively damaged plasmid DNA is impaired after delivery into human host cells not only due to decreased retention in the transfected cells, but also due to selective silencing of the damaged reporter gene. To test whether the gene silencing was associated with a specific change of the chromatin structure, we determined the levels of histone modifications related to transcriptional activation (acetylated histones H3 and H4) or repression (methylated K9 and K27 of the hi…
8-Oxoguanine DNA glycosylase (Ogg1) causes a transcriptional inactivation of damaged DNA in the absence of functional Cockayne syndrome B (Csb) prote…
2008
We have analysed the effect of oxidative guanine lesions on the expression of a transfected reporter gene in mouse embryonic fibroblasts deficient in Cockayne syndrome B protein (Csb) and/or the 8-oxoguanine DNA glycosylase (Ogg1). We used a highly sensitive flow cytometry-based approach and quantitative real-time PCR to measure the changes in gene expression caused by the presence of oxidised guanine residues generated by photosensitisation in the vector DNA. In wild-type cells, small numbers (one or three) of oxidised guanines did not affect gene expression at short times after transfections, whereas progressive reduction of the transgene expression was observed at later time points. Alth…
The sensitivity of buckwheat plants to pathogens under conditions of mixed viral and mycoplasmal infection
2016
It was investigated, that mixed infection of buckwheat distributed in various ecological regions of Ukraine, the pathogens that belong to different taxonomic groups have different and peculiar morphological and structural properties. First was shown that buckwheat is affected by buckwheat burn virus (BBV) together with Mycoplasma. Mixed infection leads to proliferation of sepals, clarification of the flowers petals, reduction and greening of petals, hypoplasia or hypodevelopment of stamens and pistils: all of this can lead to partial or complete sterility.
Green Public Procurement: Case Study of Latvian Municipalities
2019
Abstract Green public procurement (GPP) is an instrument for public institutions that have made sustainable consumption and financial aids savings, particularly taking into consideration expenses of the total life cycle of the agreement, not just the procurement price. Municipalities are important to promotion of sustainable consumption through their positive effects on citizens and other stakeholders. The present paper analyses the contribution of municipalities of the Republic of Latvia in developing sustainable consumption through GPP. The aim of the paper is to explore the development of GPP in municipalities, analysing influencing factors. By analysing data from the Procurement Supervi…
Green Bonds: Between economic incentives and eco-change
2020
Abstract As the investor base committed to financing sustainable companies in an attempt to combat the climate crisis expands, green financial products have become more attractive to issuers, corporate and sovereign alike. As a result, the EU is attempting to create favourable market conditions which mobilise the allocation of private capital for investments that reduce the contribution to climate change. As part of the EU Commission’s Action Plan for Sustainable Finance, it intends to create Green Bond Standards which aim to support the transition to greener securities investments. As a foundation, we provide an overview of the green bond market development. We then consider investment cha…
Consensus guidelines for the detection of immunogenic cell death
2014
Apoptotic cells have long been considered as intrinsically tolerogenic or unable to elicit immune responses specific for dead cell-associated antigens. However, multiple stimuli can trigger a functionally peculiar type of apoptotic demise that does not go unnoticed by the adaptive arm of the immune system, which we named "immunogenic cell death" (ICD). ICD is preceded or accompanied by the emission of a series of immunostimulatory damage-associated molecular patterns (DAMPs) in a precise spatiotemporal configuration. Several anticancer agents that have been successfully employed in the clinic for decades, including various chemotherapeutics and radiotherapy, can elicit ICD. Moreover, defect…
Synthesis of iodobiaryls and dibenzofurans by direct coupling at BDD anodes.
2014
The first direct oxidative phenol-arene cross-coupling reactions of an iodine-containing guaiacol derivative and the possible over-oxidation products of electron-rich phenols are described. Hereby, a "green" and targeted synthesis for dibenzofurans was developed.