Search results for "GRI"
showing 10 items of 10209 documents
Intake and milk production of goats grazing Sulla forage under different stocking rates
2010
This investigation aimed to individuate the dietary factors affecting the milk urea nitrogen (MUN) concentration in goats grazing herbaceous pasture and, particularly, to verify the relationship linking the diet crude protein (CP) content to MUN. A total of 205 individual observations regarding dietary and milk variables of 37 Girgentana goats involved in two experiments were used. Goats, averaging 154±14 days in milk and 38.1±5.4 kg of live weight, grazed on swards and received 500 g/d of barley meal. Sward biomass, herbage selected by goats and individual milk yield were measured and sampled weekly. The herbage intake and diet digestibility were estimated by the n-alkane method. Milk urea…
Soil carbon quality and nitrogen fertilization structure bacterial communities with predictable responses of major bacterial phyla
2014
Abstract Agricultural practices affect the soil ecosystem in multiple ways and the soil microbial communities represent an integrated and dynamic measure of soil status. Our aim was to test whether the soil bacterial community and the relative abundance of major bacterial phyla responded predictably to long-term organic amendments representing different carbon qualities (peat and straw) in combination with nitrogen fertilization levels and if certain bacterial groups were indicative of specific treatments. We hypothesized that the long-term treatments had created distinctly different ecological niches for soil bacteria, suitable for either fast-growing copiotrophic bacteria, or slow-growing…
Influence of carbon and nutrient additions on a decomposer food chain and the growth of pine seedlings in microcosms
2001
Abstract Because of N deposition plant production is becoming increasingly limited by other nutrients in boreal forests. At the same time more C is suggested to become available for below-ground food webs because of enhanced CO2 fixation. We studied the effects of carbon and nutrient addition on a fungus–nematode food chain and on the growth of mycorrhizal or nonmycorrhizal pine seedlings (Pinus sylvestris L.) in microcosms with N-rich mineral soil and a humus layer. The role of the food chain and mycorrhizal fungi for pine growth was tested in a smaller set-up. The total pine biomass was not N-limited at the scale of two experimental growing seasons. In accordance with established knowledg…
Functional diversity of decomposer organisms in relation to primary production
1998
Abstract The term `biodiversity' is claimed to lack connections to a serious scientific background. In this work, we approached the concept of biodiversity from a functional point of view by asking: “At what level of the ecological organization (species, trophic species/feeding guilds, trophic levels etc.) should reduction in biodiversity matter to bring about visible changes in ecosystem performance?” We investigate the concepts of `functional diversity' and `ecosystem performance' in relation to feeding habits (such as fungivory, detritivory etc.) of soil fauna and plant growth. After analysing the results of a number of microcosm studies, we came into the following conclusions: (i) troph…
The rotifer community and its functional role in lakes of a neotropical floodplain
2014
Aquatic communities vary depending on local factors, such as environmental conditions and biological interactions and regional factors, such as the hydrological regime in floodplains. This study analyzed the community structure of rotifers and their secondary production in two lakes of an Upper Parana River floodplain (Brazil) differing in their hydrology: one connected to the river and the other isolated. Integrated samples of the water column were collected daily during 15 days in the low and high water periods. Three statistical methods, ANOVA, MRT, and IndVal were applied to distinguish the structure, biomass, and secondary production of their rotifer communities. The rotifer fauna was …
Temperature-induced microstructural changes in shells of laboratory-grown Arctica islandica (Bivalvia).
2021
Bivalve shells are increasingly used as archives for high-resolution paleoclimate analyses. However, there is still an urgent need for quantitative temperature proxies that work without knowledge of the water chemistry–as is required for δ18O-based paleothermometry–and can better withstand diagenetic overprint. Recently, microstructural properties have been identified as a potential candidate fulfilling these requirements. So far, only few different microstructure categories (nacreous, prismatic and crossed-lamellar) of some short-lived species have been studied in detail, and in all such studies, the size and/or shape of individual biomineral units was found to increase with water temperat…
Small unilamellar liposomes from mixed natural and polymeric phospholipids: stability and susceptibility to phospholipase A2.
1991
The concept of the uncorkable liposome composed of phase-separated mixtures of a polymerized phospholipid and an enzymically digestible phospholipid has been investigated, using small unilamellar vesicles composed of mixtures of (polymerized) dienoylphosphatidylcholine (DENPC) and dimyristoylphosphatidylcholine (DMPC). Mixed liposomes, even those containing only 10% DENPC, were much more stable than DMPC liposomes, as indicated by the release of entrapped [3H]inulin or [14C]glucose. DMPC liposomes released entrapped solute on exposure to phospholipase A2, whereas mixed vesicles were resistant. The results are compared with those of an earlier study on monolayers of similar compositions. It …
Performance of polyester-based electrospun scaffolds under in vitro hydrolytic conditions: From short-term to long-term applications
2019
The evaluation of the performance of polyesters under in vitro physiologic conditions is essential to design scaffolds with an adequate lifespan for a given application. In this line, the degradation-durability patterns of poly(lactide-co-glycolide) (PLGA), polydioxanone (PDO), polycaprolactone (PCL) and polyhydroxybutyrate (PHB) scaffolds were monitored and compared giving, as a result, a basis for the specific design of scaffolds from short-term to long-term applications. For this purpose, they were immersed in ultra-pure water and phosphate buffer solution (PBS) at 37 °
Cleavage of endometrial α-integrins into their functional forms is mediated by proprotein convertase 5/6.
2012
Background Proprotein convertases (PCs) post-translationally activate a large number of protein precursors through limited cleavage. PC5/6 (PC6) in the human endometrium is tightly regulated during receptivity for embryo implantation. Integrins are transmembrane glycoproteins, some of which play an important role in the adhesive interactions between the trophoblast (blastocyst) and uterine epithelium at implantation. Integrins require PC cleavage for post-translational modification. We hypothesize that pro-integrin-αs in the endometrial epithelium are post-translationally cleaved by PC6 into functional subunits for the binding of blastocyst and adhesion of extracellular matrix proteins. Met…
αv-Class integrin binding to fibronectin is solely mediated by RGD and unaffected by an RGE mutation.
2020
Fibronectin (FN) is an essential glycoprotein of the extracellular matrix; binds integrins, syndecans, collagens, and growth factors; and is assembled by cells into complex fibrillar networks. The RGD motif in FN facilitates cell binding and fibrillogenesis through binding to α5β1 and αv-class integrins. However, whether RGD is the sole binding site for αv-class integrins is unclear. Most notably, substituting aspartate with glutamate (RGE) was shown to eliminate integrin binding in vitro, while mouse genetics revealed that FNRGE preserves αv-class integrin binding and fibrillogenesis. To address this conflict, we employed single-cell force spectroscopy, engineered cells, and RGD motif–defi…