Search results for "GRI"
showing 10 items of 10209 documents
Novel α2β1 integrin inhibitors reveal that integrin binding to collagen under shear stress conditions does not require receptor preactivation.
2012
The interaction between α2β1 integrin (GPIa/IIa, VLA-2) and vascular collagen is one of the initiating events in thrombus formation. Here, we describe two structurally similar sulfonamide derivatives, BTT-3033 and BTT-3034, and show that, under static conditions, they have an almost identical effect on α2-expressing CHO cell adhesion to collagen I, but only BTT-3033 blocks platelet attachment under flow (90 dynes/cm(2)). Differential scanning fluorimetry showed that both molecules bind to the α2I domain of the recombinant α2 subunit. To further study integrin binding mechanism(s) of the two sulfonamides, we created an α2 Y285F mutant containing a substitution near the metal ion-dependent ad…
TREM-1 ligand expression on platelets enhances neutrophil activation
2007
Abstract The triggering receptor expressed on myeloid cells 1 (TREM-1) plays an important role in the innate immune response related to severe infections and sepsis. Modulation of TREM-1–associated activation improves the outcome in rodent models for pneumonia and sepsis. However, the identity and occurrence of the natural TREM-1 ligands are so far unknown, impairing the further understanding of the biology of this receptor. Here, we report the presence of a ligand for TREM-1 on human platelets. Using a recombinant TREM-1 fusion protein, we demonstrate specific binding of TREM-1 to platelets. TREM-1–specific signals are required for the platelet-induced augmentation of polymorphonuclear leu…
High-throughput elucidation of thrombus formation reveals sources of platelet function variability
2019
In combination with microspotting, whole-blood microfluidics can provide high-throughput information on multiple platelet functions in thrombus formation. Based on assessment of the inter-and intra-subject variability in parameters of microspot-based thrombus formation, we aimed to determine the platelet factors contributing to this variation. Blood samples from 94 genotyped healthy subjects were analyzed for conventional platelet phenotyping: i.e. hematologic parameters, platelet glycoprotein (GP) expression levels and activation markers (24 parameters). Furthermore, platelets were activated by ADP, CRP-XL or TRAP. Parallel samples were investigated for whole-blood thrombus formation (6 mi…
The first comprehensive and quantitative analysis of human platelet protein composition allows the comparative analysis of structural and functional …
2012
AbstractAntiplatelet treatment is of fundamental importance in combatting functions/dysfunction of platelets in the pathogenesis of cardiovascular and inflammatory diseases. Dysfunction of anucleate platelets is likely to be completely attributable to alterations in posttranslational modifications and protein expression. We therefore examined the proteome of platelets highly purified from fresh blood donations, using elaborate protocols to ensure negligible contamination by leukocytes, erythrocytes, and plasma. Using quantitative mass spectrometry, we created the first comprehensive and quantitative human platelet proteome, comprising almost 4000 unique proteins, estimated copy numbers for …
Griffonianone D, an isoflavone with anti-inflammatory activity from the root bark of Millettia griffoniana.
2003
A new isoflavone, griffonianone D (1), and the previously known compounds durmillone and odorantin were isolated from a chloroform extract of the root bark of Millettia griffoniana. The structure of 1 was established as (7E)-(6",7"-dihydroxy-3",7"-dimethyloct-2"-enyl)oxy-4'-methoxyisoflavone on the basis of its spectral data. The chloroform extract of the root bark of M. griffoniana and compound 1 showed anti-inflammatory effects in different experimental models of inflammation.
Enhancement of immunohistochemical staining
1982
The present paper describes two simple procedures which enhance immunohistochemical staining. One is to cover the sections with a plastic film to keep the serum uniformly distributed and minimize its evaporation. Secondly, rocking of the slides has been introduced, causing the serum to flow back and forth under the plastic film. Using this system, it has been possible to test for the effect of mixing on an immunohistochemical reaction (the demonstration of calcitonin in thyroid C cells). It has been found that mixing definitely enhances the reaction during the first 8 h. No effect of serum volume was observed.
Shortstop Recruits EB1/APC1 and Promotes Microtubule Assembly at the Muscle-Tendon Junction
2003
Abstract Background: Shot (previously named Kakapo), is a Drosophila Plakin family member containing both Actin binding and microtubule binding domains. In Drosophila , it is required for a wide range of processes, including axon extension, dendrite formation, axonal terminal arborization at the neuromuscular junction, tendon cell development, and adhesion of wing epithelium. Results: To address how Shot exerts its activity at the molecular level, we investigated the molecular interactions of Shot with candidate proteins in mature larval tendon cells. We show that Shot colocalizes with EB1/APC1 and with a compact microtubule array extending between the muscle-tendon junction and the cuticle…
Comparison between rectal and body surface temperature in dogs by the calibrated infrared thermometer
2020
Highlights • Dogs poorly tolerate rectal temperature measurements with a contact thermometer. • Existing alternative approaches used uncalibrated infrared thermometers. • Gum and inguinal temperature are correlated moderately to rectal temperature. • Hyperthermia was detected with sensitivity and specificity up to 90.0% and 78.6%. • Future studies should include a calibrated thermometer and control external factors.
Microremains from El Mirón Cave human dental calculus suggest a mixed plant/animal subsistence economy during the Magdalenian in Northern Iberia
2015
Abstract Despite more than a century of detailed investigation of the Magdalenian period in Northern Iberia, our understanding of the diets during this period is limited. Methodologies for the reconstruction of Late Glacial subsistence strategies have overwhelmingly targeted animal exploitation, thus revealing only a portion of the dietary spectrum. Retrieving food debris from calculus offers a means to provide missing information on other components of diet. We undertook analysis of human dental calculus samples from Magdalenian individuals (including the “Red Lady”) at El Miron Cave (Cantabria, Spain), as well as several control samples, to better understand the less visible dietary compo…
Sheep and Cattle Reservoirs in the Highest Human Fascioliasis Hyperendemic Area: Experimental Transmission Capacity, Field Epidemiology, and Control …
2020
The Northern Bolivian Altiplano is the human fascioliasis hyperendemic area where the highest prevalences and intensities of infection by Fasciola hepatica in humans have been reported. Four animal species are the reservoir species for F. hepatica in this area, namely, sheep, cattle, pigs, and donkeys. Livestock for the Aymara inhabitants is crucial because vegetable cultures are not viable due to the inhospitality of the very high altitude of 3,820–4,100 m. A One Health initiative has been implemented in this area in recent years, as the first such control action in a human endemic area ever. Among the different control axes included, special focus is devoted to the two main reservoirs she…