Search results for "Genus"

Using DNA barcodes to detect non-indigenous species: the case of the Asian copepod Mesocyclops pehpeiensis Hu, 1943 (Cyclopidae) in two regions of th…

2015

To date, little attention has been paid to analyses of copepods as exotic species. The genusMesocyclops, a freshwater cyclopoid, has a worldwide distribution, but individual species within the genus have a quite restricted geographical range.Mesocyclops pehpeiensisHu, 1943 is a Central-East Asian species, rarely found outside of this area, and when it appears should be considered as non-native. Based on morphology and DNA barcode analyses, using the COI gene, we confirmed records ofM. pehpeiensisin two ponds in Mexico and in a rice paddy near Valencia, Spain. The morphology of this species, based on morphometric analyses, was found to be variable, but DNA barcoding confirmed the same identi…

Evaluation of genetic variability and relatedness among eight Centaurea species through CAAT-box derived polymorphism (CBDP) and start codon targeted…

2021

Centaurea is a value-ultimate genus of medicinal plants showing high diversification levels, especially within the Mediterranean basin, and is still traditionally recognized as a complicated taxon. So far, few studies utilizing molecular markers have been done on Centaurea spp. towards a better dissection of its phylogeny and accurate assessment of genetic diversity. Here, two functional marker systems, start codon targeted (SCoT) polymorphism and CAAT box-derived polymorphism (CBDP), were implemented to assess the genetic diversity between eight wild Centaurea species in Egypt. Seventeen SCoT and 19 CBDP primers generated 197 and 179 bands, respectively. These primers generated 158 (80.2%)…

New chromosome numbers for plant taxa endemic to the Balearic Islands

2006

Mitotic chromosome numbers are reported from 25 vascular plant taxa, endemic to the Balearic Islands that are poorly known cytogenetically. The chromosome numbers ofAnthyllis vulneraria subsp.balearica (2n=12),Cymbalaria fragilis (2n=56), andPolygonum romanum subsp.balearicum (2n=40) were determined for the first time. A new chromosome number was found in several populations ofAnthyllis hystrix (2n=70) suggesting that this species is decaploid, in contrast to an earlier work reporting a higher ploidy level (2n=12x=84). The new chromosome number 2n=32 was reported inHypericum hircinum subsp.cambessedesii. It is suggested that the previous count (2n=40) could be explained by the presence of a…

Centromere-Like Elements in Megaselia Spiracularis (Diptera: Phoridae): A Fine-Structure and Cytogenetic Study

2004

The present study was concerned with the karyotype of a humpbacked fly, Megaselia spiracularis (Diptera: Phoridae). Chromosome preparations of neuroblasts isolated from male and female pupae uniformly showed two pairs of metacentric chromosomes and a telocentric chromosome pair. All chromosomes were homomorphic. The analysis of ultrathin serial sections through spermatogonia in metaphase using transmission electron microscopy, confirmed the presence of 3 regular chromosome pairs. In ultrathin sections, the centromeres could be detected as individual elements owing to their lesser electron density in comparison with the chromosome arms. In addition, the spindles contained two tiny elements s…

Genetic analysis of heat shock response in three Drosophila species of the obscura group

1992

Heat shock response was investigated in three species of the obscura group of the Drosophila genus (D. subobscura, D. guanche, and D. madeirensis) by chromosome cytology analysis and [3H]uridine labeling. A set of eight puffs (2C, 15DE, 18C, 27A, 31CD, 85AB, 89A, and 94A) were induced after heat treatments in each of the three species; 18C, 27A, 89A, and 94A were the most heavily labeled in the autoradiograms after the induced conditions. From the in situ results using the major heat shock genes of D. melanogaster as a probe, it was inferred that the 18C, 94A, 89A, and 27A loci of the three obscura group species are homologous to D. melanogaster loci, which contain, HSP82, HSP70, HSP68, an…

Populations, hybrids and the systematic concepts of species and subspecies in Chagas disease triatomine vectors inferred from nuclear ribosomal and m…

2009

In Chagas disease, triatomine vectors are the main target for control measures because of the absence of effective drugs. The broad usefulness of nuclear rDNA and mtDNA sequences explains why triatomine studies using these markers have increased so pronouncedly in recent years. This indicates the appropriateness of an updated review about these molecular markers, concentrating on aspects useful for research on Chagas disease vectors. A comparative analysis is presented on the efficiency, weight of their different characteristics, limitations and problems of each of the different DNA markers in the light of the results obtained in studies on populations, hybrids, subspecies and species of th…

Rapid characterization of wild and collection strains of the genus Zygosaccharomyces according to mitochondrial DNA patterns

1997

Several wild and collection strains of the genus Zygosaccharomyces were characterized using a rapid and simple method of restriction analysis of mitochondrial DNA. Patterns obtained with three endonucleases (HaeIII, HinfI and RsaI) made it possible to differentiate each species and to identify the wild strains, isolated from the same spoiled concentrated must, as belonging to the species Z. rouxii. The HinfI restriction enzyme produced a strain-specific pattern which allowed us to recognize that the seven wild isolates belonged to only three strains.

P2‐077: Identification of potential modifiers of Alzheimer's disease pathology by quantitative mass spectrometry and drosophila genetics

2015

Molecular characterisation of the species of the genus Zygosaccharomyces.

2003

The restriction fragments polymorphisms of the mitochondrial DNA and the PCR fragment that comprised the internal transcribes spacers and the 5.8S rRNA gene, together with the electrophoretic karyotypes of 40 strains from the 10 species of the genus Zygosaccharomyces, including the new species Z. lentus were examined. The RFLP's of the ITS-5.8S region showed a specific restriction pattern for each species, including the new species Z. lentus. The only exception were the species Z. cidri and Z. fermentati that produced identical restriction profiles. The electrophoretic chromosome patterns confirmed the differences between the species of this genus, including the phylogenetic closest species…

Evolution of gypsy Endogenous Retrovirus in the Drosophila obscura Species Group

2000

The Ty3/gypsy family of retroelements is closely related to retroviruses, and some of their members have an open reading frame resembling the retroviral gene env. Sequences homologous to the gypsy element from Drosophila melanogaster are widely distributed among Drosophila species. In this work, we report a phylogenetic study based mainly on the analysis of the 5' region of the env gene from several species of the obscura group, and also from sequences already reported of D. melanogaster, Drosophila virilis, and Drosophila hydei. Our results indicate that the gypsy elements from species of the obscura group constitute a monophyletic group which has strongly diverged from the prototypic D. m…