Search results for "Gram"
showing 10 items of 9069 documents
Specimens at the Center: An Informatics Workflow and Toolkit for Specimen-level analysis of Public DNA database data
2016
Pham, Kasey K. [et al.]
Biochemical characterization of the skeletal matrix of the massive coral, Porites australiensis - The saccharide moieties and their localization.
2018
11 pages; International audience; To construct calcium carbonate skeletons of sophisticated architecture, scleractinian corals secrete an extracellular skeletal organic matrix (SOM) from aboral ectodermal cells. The SOM, which is composed of proteins, saccharides, and lipids, performs functions critical for skeleton formation. Even though polysaccharides constitute the major component of the SOM, its contribution to coral skeleton formation is poorly understood. To this end, we analyzed the SOM of the massive colonial coral, Porites australiensis, the skeleton of which has drawn great research interest because it records environmental conditions throughout the life of the colony. The coral …
The chaperone-like protein Cdc48 regulates ubiquitin-proteasome system in plants.
2021
The degradation of misfolded proteins is mainly mediated by the ubiquitin-proteasome system (UPS). UPS can be assisted by the protein Cdc48 but the relationship between UPS and Cdc48 in plants has been poorly investigated. Here, we analyzed the regulation of UPS by Cdc48 in tobacco thanks to two independent cell lines overexpressing Cdc48 constitutively and plant leaves overexpressing Cdc48 transiently. In the cell lines, the accumulation of ubiquitinated proteins was affected both quantitatively and qualitatively and the amount of proteasomal subunits was modified, while proteolytic activities were unchanged. Similarly, the over-expression of Cdc48 in planta impacted the accumulation of ub…
Emendation of Rules 5b, 8, 15 and 22 of the International Code of Nomenclature of Prokaryotes to include the rank of phylum.
2021
Following the International Committee on Systematics of Prokaryotes electronic discussion and vote on proposals to include the rank of phylum in the rules of the International Code of Nomenclature of Prokaryotes, we here announce the results of the ballot. We also present draft versions of the emended Rules 5b, 8, 15 and 22, based on the outcome of the ballot, to be included in the proposal for the preparation of a new revision of the International Code of Nomenclature of Prokaryotes.
Proteomic insights into the immune response of the Colorado potato beetle larvae challenged with Bacillus thuringiensis.
2019
Bacillus thuringiensis (Bt) toxins constitute effective, environmentally safe biopesticides. Nevertheless, insects' tolerance to Bt is influenced by environmental factors affecting immunity. To understand larval immune response in the devastating coleopteran insect pest Colorado potato beetle (CPB), we undertook a proteomic analysis of hemolymph of non-treated control larvae and larvae consuming non-lethal doses of spore-crystal mixtures containing the coleopteran-active Cry3Aa toxin. Results revealed lower amount of proteins involved in insect growth and higher amount of immune response-related proteins in challenged insects, sustaining the larval weight loss observed. Additionally, we fou…
Functional characterization of the chaperon-like protein Cdc48 in cryptogein-induced immune response in tobacco
2017
SPEIPMUBINRASUPDATDOCT; Cdc48, a molecular chaperone conserved in different kingdoms, is a member of the AAA+ family contributing to numerous processes in mammals including proteins quality control and degradation, vesicular trafficking, autophagy and immunity. The functions of Cdc48 plant orthologues are less understood. We previously reported that Cdc48 is regulated by S-nitrosylation in tobacco cells undergoing an immune response triggered by cryptogein, an elicitin produced by the oomycete Phytophthora cryptogea. Here, we inv estigated the function of NtCdc48 in cryptogein signalling and induced hypersensitive-like cell death. NtCdc48 was found to accumulate in elicited cells at both th…
An integrated proteomic and metabolomic study to evaluate the effect of nucleus-cytoplasm interaction in a diploid citrus cybrid between sweet orange…
2018
Key message: Our results provide a comprehensive overview how the alloplasmic condition might lead to a significant improvement in citrus plant breeding, developing varieties more adaptable to a wide range of conditions. Abstract: Citrus cybrids resulting from somatic hybridization hold great potential in plant improvement. They represent effective products resulting from the transfer of organelle-encoded traits into cultivated varieties. In these cases, the plant coordinated array of physiological, biochemical, and molecular functions remains the result of integration among different signals, which derive from the compartmentalized genomes of nucleus, plastids and mitochondria. To dissect …
Boosting Biomass Quantity and Quality by Improved Mixotrophic Culture of the Diatom Phaeodactylum tricornutum
2021
Diatoms are photoautotrophic unicellular algae and are among the most abundant, adaptable, and diverse marine phytoplankton. They are extremely interesting not only for their ecological role but also as potential feedstocks for sustainable biofuels and high-value commodities such as omega fatty acids, because of their capacity to accumulate lipids. However, the cultivation of microalgae on an industrial scale requires higher cell densities and lipid accumulation than those found in nature to make the process economically viable. One of the known ways to induce lipid accumulation in Phaeodactylum tricornutum is nitrogen deprivation, which comes at the expense of growth inhibition and lower c…
100‐million‐year‐old ant–conifer associates inside French amber: a fortuitous or ecological association?
2020
International audience; Ants exhibit a plethora of ecological interactions with terrestrial plants. These interactions are broadly surveyed in modern ecosystems, but are much more difficult to unveil in the fossil record. Here, we report a unique ant–conifer association preserved in an opaque piece of 100‐million‐year‐old amber from Charentes in Western France, revealed by propagation phase‐contrast X‐ray synchrotron microtomography (PPC‐SRμCT). Most legs of the ant encircle the conifer twig, and the arthropod harbours a hooked position onto the leafy axis. The conifer is assigned to Glenrosa carentonensis Moreau, Néraudeau, Tafforeau and Dépré, whereas the ant is ascribed to Gerontoformica…
Prevention of Fusarium head blight infection and mycotoxins in wheat with cut-and-carry biofumigation and botanicals
2020
Fusarium head blight (FHB) is a devastating fungal disease of wheat worldwide causing yield losses and grain contamination with mycotoxins that jeopardise food and feed safety. Field experiments using mulch layers or botanicals were conducted in two consecutive years to investigate prevention measures with the potential to suppress FHB and reduce mycotoxins in wheat. We simulated a system with high disease pressure, i.e. maize-wheat rotation under no-tillage, by applying maize residues artificially inoculated with Fusarium graminearum in field plots after wheat sowing. For mulch layers, a novel cut-and-carry biofumigation approach was employed. Cover crops grown in separate fields were harv…