Search results for "HAp"

In situ analysis of the bacterial communities associated to farmed eel by whole-cell hybridization.

1999

Bacterial communities in water samples and eel slime were investigated by fluorescence in situ hybridization of whole bacterial cells in an eel intensive culture system over 1 year. A newly developed probe, matching 27 Vibrio spp., and a specific probe for Vibrio vulnificus were used. Phylogenetic probes complementary to selected regions of the 16S and 23S ribosomal RNA revealed that Proteobacteria of the alpha and beta subclass were predominant in water and eel slime. Members of the gamma subclass (e.g. vibrios and aeromonads) were more abundant in eel slime, although no V. vulnificus was detected.

2004

Background Arabidopsis thaliana copper metallochaperone CCH is a functional homologue of yeast antioxidant ATX1, involved in cytosolic copper transport. In higher plants, CCH has to be transported to specialised cells through plasmodesmata, being the only metallochaperone reported to date that leaves the cell where it is synthesised. CCH has two different domains, the N-terminal domain conserved among other copper-metallochaperones and a C-terminal domain absent in all the identified non-plant metallochaperones. The aim of the present study was the biochemical and biophysical characterisation of the C-terminal domain of the copper metallochaperone CCH.

Recovery of Endogenous β-Cell Function in Nonhuman Primates After Chemical Diabetes Induction and Islet Transplantation

2008

OBJECTIVE—To describe the ability of nonhuman primate endocrine pancreata to reestablish endogenous insulin production after chemical β-cell destruction. RESEARCH DESIGN AND METHODS—Eleven monkeys (Macaca fascicularis) were rendered diabetic with streptozotocin. Eight diabetic monkeys received intraportal porcine islet transplantation. RESULTS—Two monkeys transplanted after 75 days of type 1 diabetes showed recovery of endogenous C-peptide production a few weeks after transplantation, concomitant with graft failure. Histological analysis of the pancreas of these monkeys showed insulin-positive cells, single or in small aggregates, scattered in the pancreas and adjacent to ducts. Interesting…

Context dependent variation in corticosterone and phenotypic divergence of Rana arvalis populations along an acidification gradient

2022

Background Physiological processes, as immediate responses to the environment, are important mechanisms of phenotypic plasticity and can influence evolution at ecological time scales. In stressful environments, physiological stress responses of individuals are initiated and integrated via the release of hormones, such as corticosterone (CORT). In vertebrates, CORT influences energy metabolism and resource allocation to multiple fitness traits (e.g. growth and morphology) and can be an important mediator of rapid adaptation to environmental stress, such as acidification. The moor frog, Rana arvalis, shows adaptive divergence in larval life-histories and predator defense traits along an acidi…

Tissue-Specific Dynamics in the Endophytic Bacterial Communities in Arctic Pioneer Plant

2020

The rapid developments in the next-generation sequencing methods in the recent years have provided a wealth of information on the community structures and functions of endophytic bacteria. However, the assembly processes of these communities in different plant tissues are still currently poorly understood, especially in wild plants in natural settings. The aim of this study was to compare the composition of endophytic bacterial communities in leaves and roots of arcto-alpine pioneer plant Oxyria digyna, and investigate, how plant tissue (leaf or root) or plant origin affect the community assembly. To address this, we planted micropropagated O. digyna plants with low bacterial load (bait pla…

Molecular docking and oxidation kinetics of 3-phenyl coumarin derivatives by human CYP2A13

2021

CYP2A13 enzyme is expressed in human extrahepatic tissues, while CYP2A6 is a hepatic enzyme. Reactions catalysed by CYP2A13 activate tobacco-specific nitrosamines and some other toxic xenobiotics in lungs.To compare oxidation characteristics and substrate-enzyme active site interactions in CYP2A13 vs CYP2A6, we evaluated CYP2A13 mediated oxidation characteristics of 23 coumarin derivatives and modelled their interactions at the enzyme active site.CYP2A13 did not oxidise six coumarin derivatives to corresponding fluorescent 7-hydroxycoumarins. The Km-values of the other coumarins varied 0.85���97 ��M, Vmax-values of the oxidation reaction varied 0.25���60 min���1, and intrinsic clearance var…

Fondements et approches de modélisation 3D des environnements urbains pour la géosimulation

2015

National audience; Dans le prolongement de la théorie de la géographie virtuelle proposée par Mi¬chael Batty (1997, 2008), les Environne¬ments Géographiques Virtuels (EGVs) sont proposés comme une nouvelle génération d’outils d’analyses géographiques, d’amé¬nagements et de prospective de façon plus approfondie (Lin et al., 2012). Le déve¬loppement des EGVs répond aux trois exigences scientifiques fondamentales des sciences de l’information géographique : vi-sualisation multidimensionnelle, simulation dynamique de phénomènes géographiques et la participation collaborative pluridisci¬plinaire (Goodchild, 2009). A ce titre, les Environnements Virtuels Urbains pour la Géosimulation (EVUG) sont …

Microwave-Assisted Esterification of Tall Oil Fatty Acids with Methanol Using Lignin-Based Solid Catalyst

2016

During alkaline pulping significant amounts of lignin, carbohydrates (mostly hemicelluloses), and extractives (tall oil soap and turpentine) are removed from wood feedstock. In this study, the catalytic esterification of fatty acids in tall oil with methanol to produce fatty acid methyl esters under microwave irradiation was performed at 100 °C for 10–60 min. A novel heterogeneous acid catalyst tested for this purpose was synthesized from the hardwood alkali lignin that was precipitated by acidification from the black liquor from soda-AQ pulping. The comparative reaction data were obtained by using other solid catalysts, Amberlyst 15, and p-toluenesulfonic acid. The results showed the highe…

Taylorin sääntö ja Euroopan keskuspankin rahapolitiikka : onko euroalueen velkakriisi vaikuttanut EKP:n harjoittamaan rahapolitiikkaan?

2013

Tässä tutkielmassa selvitetään, noudattaako Euroopan keskuspankki Taylorin sääntöä rahapoliittisia päätöksiä tehdessään. Lisäksi tarkastellaan, onko euroalueen velkakriisi vaikuttanut Euroopan keskuspankin harjoittamaan rahapolitiikkaan. Taylorin (1993) mukaan keskuspankit huomioivat korkoja asettaessaan kotimaisen inflaation ja tuotannon poikkeamat niille asetetuista tavoitteista. Tarkasteluajanjakso alkaa euroalueen yhteisen rahapolitiikan käyttöönotosta tammikuussa 1999 ulottuen joulukuuhun 2012. Aineisto on koottu Euroopan keskuspankin pääasiassa reaaliaikaisen aineiston tietokannasta sekä OECD:n verkkosivuilta. Toteutettavassa tutkimuksessa estimoidaan senhetkisiä ja eteenpäin katsovia…

Effects of exercise training on maximal oxygen uptake in heart failure : a systematic review and meta-analysis

2012

SUMMARY Aims. Low cardiorespiratory fitness is a common physical status among patients with heart failure. Several studies have examined the effects of exercise training on maximal oxygen uptake (VO2max) in heart failure. Though, these studies had relatively small sample sizes and highly variable results. Therefore the aim of this study was to systemically review the effects of exercise training on VO2max in heart failure patients. Methods. Database search of randomized controlled trials was conducted from Ovid MEDLINE and Cochrane Central Register of Controlled Trials. Search was allocated to studies with patients with heart failure, structured exercise intervention, control group receivin…