Search results for "HOST"

Development of functionalized SYBR green II related cyanine dyes for viral RNA detection

2020

Fluorescent probes for sensing nucleic acids have found widespread use in the field of cell and molecular biology. However, probes combined with potential for post-synthetic conjugation, e.g. for intra-endosomal measurements of RNA, are unavailable. Herein we developed cyanine dyes that can be conjugated to viral capsid or other targets. First, we solved the crystal structure of SYBR Green II. The structural elucidation of this commonly used RNA probe provided the basis for synthesizing similar molecules with much desired function for post-synthetic conjugation. To address this need, cyanine dyes were prepared using an alternative synthesis protocol. All studied compounds showed considerabl…

Depletion of

2020

Child obesity constitutes a risk factor for developing insulin resistance which, if sustained, could lead to more severe conditions like type 2 diabetes (T2D) in adulthood. Our study identified previously unknown species whose depletion (Blautia luti and Blautia wexlerae) is associated with insulin resistance in obese individuals. Our results also indicate that these bacterial species might help to reduce inflammation causally linked to obesity-related complications. Childhood is considered a window of opportunity to tackle obesity. These new findings provide, therefore, valuable information for the future design of microbiota-based strategies for the early prevention of obesity-related com…

Ohjelmistokehittäjän keinoja Java-koodin tehostamiseen ja keinojen hyödyllisyys

2010

 Honkanen, Juho Ville-Pekka Ohjelmistokehittäjän keinoja Java-koodin tehostamiseen ja keinojen hyödyllisyys / Ville-Pekka Honkanen Jyväskylä: Jyväskylän yliopisto, 2010. 37 s. Kandidaatintutkielma Tässä tutkielmassa käydään lävitse keinoja, joilla voidaan parantaa Java-koodin suorituskykyä. Tutkielma on toteutettu kirjallisuuskatsauksena ja sen tarkoituksena on tutkia perinteisiä koodioptimointeja ja selvitetään onko niistä enää nykypäivänä hyötyä. Lisäksi käsitellään keinoja, joilla todella voi parantaa ohjelman suorituskykyä ja missä tilanteissa näitä voidaan käyttää. Läpi käydään myös koodaamistapoja, joita tulisi välttää, esimerkiksi lopetusmetodien käyttö. Tutkielmassa selviää, että us…

Editorial: Zoonotic Diseases: Their Host and Vectors

2021

Oral squamous cell carcinoma in a patient affected by graft-versus-host disease 20 years after allogeneic hematopoietic stem cell transplantation: a …

2020

Intensified job demands, stress of conscience and nurses' experiences during organizational change

2022

Background:Nurses frequently face ethically demanding situations in their work, and these may lead to stress of conscience. Working life is currently accelerating and job demands are intensifying. These intensified job demands include (1) work intensification, (2) intensified job-related planning demands, (3) intensified career-related planning demands, and (4) intensified learning demands. At the same time, many healthcare organizations are implementing major organizational changes that have an influence on personnel.Aim:The aim of the study was to investigate the association between intensified job demands and stress of conscience, and whether their association is moderated by organizatio…

Heart Rate Variability Monitoring During Strength and High-Intensity Interval Training Overload Microcycles

2019

Objective: In two independent study arms, we determine the effects of strength training (ST) and high-intensity interval training (HIIT) overload on cardiac autonomic modulation by measuring heart rate (HR) and vagal heart rate variability (HRV). Methods: In the study, 37 well-trained athletes (ST: 7 female, 12 male; HIIT: 9 female, 9 male) were subjected to orthostatic tests (HR and HRV recordings) each day during a 4-day baseline period, a 6-day overload microcycle, and a 4-day recovery period. Discipline-specific performance was assessed before and 1 and 4 days after training. Results: Following ST overload, supine HR, and vagal HRV (Ln RMSSD) were clearly increased and decreased (small …

Evolutionary Dynamics of Pathoadaptation Revealed by Three Independent Acquisitions of the VirB/D4 Type IV Secretion System in Bartonella

2017

The α-proteobacterial genus Bartonella comprises a group of ubiquitous mammalian pathogens that are studied as a model for the evolution of bacterial pathogenesis. Vast abundance of two particular phylogenetic lineages of Bartonella had been linked to enhanced host adaptability enabled by lineage-specific acquisition of a VirB/D4 type IV secretion system (T4SS) and parallel evolution of complex effector repertoires. However, the limited availability of genome sequences from one of those lineages as well as other, remote branches of Bartonella has so far hampered comprehensive understanding of how the VirB/D4 T4SS and its effectors called Beps have shaped Bartonella evolution. Here, we repor…

Spore-forming parasites infecting muscles of freshwater fishes : ecology and epidemiology

2017

Fish parasites may potentially harm fisheries and aquaculture. Infected fish are unusable, even though they would not pose a direct risk to human health. The aim of this thesis was to investigate the ecology and epidemiology of three previously unknown or poorly known spore-forming parasites infecting muscles of economically important fish species. The first novel microsporidian species, here described as Microsporidium luciopercae, was found from pike-perch (Sander lucioperca) and European perch (Perca fluviatilis) and formed opaque-looking patches in the muscles. The second novel microsporidian species, Myosporidium spraguei, was found from pike-perch and burbot (Lota lota) and occurred w…

MILKER’S NODULE. A PERPLEXING FARMYARD INFECTION AND THREAT TO THE IMMUNOCOMPROMISED

2017

Milker’s nodules, also called paravaccinia, is a DNA virus transmitted from infected cows to humans. It results from contact with cattle, cattle byproducts, or fomites. Classified as an occupational disorder, those at risk of exposure include farmers, butchers, and agricultural tourists. The viral infection begins 5—15 days after inoculation as an erythematous-purple, round nodule with a clear depressed center, and a surrounding erythematous ring. While familiar to those in farming communities, the presence of the nodule may be concerning to others, particularly the immunosuppressed. Milker’s nodules are selflimited in immunocompetent individuals and heal without scarring within 8 weeks. An…