Search results for "HT"

showing 10 items of 20594 documents

Flashing light emitting diodes (LEDs) induce proteins, polyunsaturated fatty acids and pigments in three microalgae

2020

As the periodic emission of light pulses by light emitting diodes (LEDs) is known to stimulate growth or induce high value biocompounds in microalgae, this flashing light regime was tested on growth and biochemical composition of the microalgae Nannochloropsis gaditana, Koliella antarctica and Tetraselmis chui. At low flashing light frequencies (e.g., 5 and 50 Hz, Duty cycle = 0.05), a strain-dependent growth inhibition and an accumulation of protein, polyunsaturated fatty acids, chlorophyll or carotenoids (lutein, β-carotene, violaxanthin and neoxanthin) was observed. In addition, a 4-day application of low-frequency flashing light to concentrated cultures increased productivities of eicos…

0106 biological sciences0301 basic medicinePigmentsLuteinBio Process EngineeringTotal lipidsSettore ING-IND/25 - Impianti ChimiciBioengineering01 natural sciencesApplied Microbiology and Biotechnology03 medical and health scienceschemistry.chemical_compoundPigment:Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Plantefysiologi: 492 [VDP]NeoxanthinPulsed lightChlorophytaVDP::Teknologi: 500::Bioteknologi: 590010608 biotechnologyVDP::Technology: 500::Biotechnology: 590MicroalgaeFood scienceBiomassCarotenoidVLAGchemistry.chemical_classificationDuty cycleDuty cycle Pigments PUFA Pulsed light Total lipidsFatty Acidsfood and beveragesGeneral Medicine:Matematikk og Naturvitenskap: 400::Basale biofag: 470::Molekylærbiologi: 473 [VDP]Flashing030104 developmental biologychemistryChlorophyllvisual_artvisual_art.visual_art_mediumFatty Acids Unsaturated:Teknologi: 500::Bioteknologi: 590 [VDP]StramenopilesPUFABiotechnologyPolyunsaturated fatty acidViolaxanthin
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Evolution of the Dentition in Holocephalans (Chondrichthyes) Through Tissue Disparity

2020

Abstract The Holocephali is a major group of chondrichthyan fishes, the sister taxon to the sharks and rays (Elasmobranchii). However, the dentition of extant holocephalans is very different from that of the elasmobranchs, lacking individual tooth renewal, but comprising dental plates made entirely of self-renewing dentine. This renewal of all tissues occurs at the postero-lingual plate surface, as a function of their statodont condition. The fossil record of the holocephalans illuminates multiple different trends in the dentition, including shark-like teeth through to those with dentitions completely lacking individual teeth. Different taxa illustrate developmental retention of teeth but w…

0106 biological sciences0301 basic medicinePlant Science010603 evolutionary biology01 natural sciences03 medical and health sciencesCalcification Physiologicstomatognathic systemExtant taxonElasmobranchiiAnimalsDentitionFossil RecordbiologyDentitionFishesBiological evolutionAnatomybiology.organism_classificationBiological EvolutionChondrichthyesHolocephalistomatognathic diseases030104 developmental biologyAnimal Science and ZoologyWear resistantToothIntegrative and Comparative Biology
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Effect of fruit-set time on the quality performance of Anona cherimola Mill. fruit in south italy

2019

Abstract The aim of this work was to analyse the differences between fruits obtained by different dates of fruit set and identify the proper fruit-set time that allows to obtain the optimum fruit quality in Annona cherimola cv Fino de Jete, planted in Southern Italy. Six fruit-set dates were selected from the first week of June to the second week of July. Fruits were sampled from October to November, when 1500 Growing Degree Days (DD) were accumulated; The DD were calculated using a base temperature of 12 °C. Fruit fresh weight (FW) and skin colour were measured. These parameters were measured also after storage, and in addition, the following parameters were measured: fruit shape (FS), pul…

0106 biological sciences0301 basic medicinePulp (paper)food and beveragesTitratable acidAnnona cherimolaGrowing degree-dayHorticultureBiologyengineering.materialbiology.organism_classificationAscorbic acid01 natural sciencesQuality performanceFruit set03 medical and health sciencesHorticulture030104 developmental biologyDry weightengineering010606 plant biology & botanyScientia Horticulturae
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Sugar exchanges in arbuscular mycorrhiza: RiMST5 and RiMST6, two novel Rhizophagus irregularis monosaccharide transporters, are involved in both suga…

2016

SPE IPM INRA UB CT1; International audience; Arbuscular mycorrhizal (AM) fungi are associated with about 80% of land plants. AM fungi provide inorganic nutrients to plants and in return up to 20% of the plant-fixed CO2 is transferred to the fungal symbionts. Since AM fungi are obligate biotrophs, unraveling how sugars are provided to the fungus partner is a key for understanding the functioning of the symbiosis. In this study, we identified two new monosaccharide transporters from Rhizophagus irregularis (RiMST5 and RiMST6) that we characterized as functional high affinity monosaccharide transporters. RiMST6 was characterized as a glucose specific, high affinity H(+) co-transporter. We prov…

0106 biological sciences0301 basic medicineRhizophagus irregularisLightPhysiology[SDV]Life Sciences [q-bio]Plant Sciencearbuscular mycorrhizal fungus01 natural sciencesrhizophagus irregularisGlomeromycotaSoilGene Expression Regulation PlantMycorrhizaeMedicagoPhylogeny2. Zero hungerMutualism (biology)Fungal proteinReverse Transcriptase Polymerase Chain Reactionglucose specificMonosaccharidesfood and beverageshigh affinity H+ co-transporterhigh affinity transporterArbuscular mycorrhizaBiochemistry[SDE]Environmental SciencesFungusSaccharomyces cerevisiaeBiologyFungal Proteins03 medical and health sciencesSymbiosisStress PhysiologicalBotanyGenetics[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyRNA MessengerGlomeromycotaObligateCell MembraneGenetic Complementation TestfungiMST5MST6Membrane Transport Proteins15. Life on landmonosaccharide transporterbiology.organism_classification030104 developmental biologyGlucose010606 plant biology & botany
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Selection of indigenous yeast strains for the production of sparkling wines from native Apulian grape varieties.

2018

We report the first polyphasic characterization of native Saccharomyces cerevisiae in order to select candidate strains for the design of starter cultures tailored for Apulian sparkling wines obtained from local grape variety. In addition, it is the first survey in our region that propose the selection of autochthonous starter cultures for sparkling wine i) including a preliminary tailored genotypic and technological screening, and ii) monitoring analytical contribution during secondary fermentation in terms of volatile compounds (VOCs). Furthermore, we exploit the potential contribute of autochthonous cultures throughout the productive chain, including the possible improvement of base wine…

0106 biological sciences0301 basic medicineSparkling wine production030106 microbiologyWineSaccharomyces cerevisiaeAutochthonous grape varietiesrr01 natural sciencesMicrobiologyInterdelta03 medical and health sciencesStarterSparkling wine010608 biotechnologyVitisFood scienceSelection (genetic algorithm)Fermentation in winemakingWineindigenous yeastsbiologyautochthonous starter culturesfood and beveragesGeneral Medicinebiology.organism_classificationggYeastFermentationFood MicrobiologyFermentationFood ScienceBiotechnologyInternational journal of food microbiology
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Oxygen Availability during Growth Modulates the Phytochemical Profile and the Chemo-Protective Properties of Spinach Juice.

2018

Fruits and vegetables are a good source of potentially biologically active compounds. Their regular consumption in the human diet can help reduce the risk of developing chronic diseases such as cardiovascular diseases and cancer. Plants produce additional chemical substances when subject to abiotic stress or infected by microorganisms. The phytochemical profile of spinach leaves (Spinacia oleracea L.), which is a vegetable with widely recognized health-promoting activity, has been affected by applying root hypoxic and re-oxygenation stress during plant growth. Leaf juice at different sampling times has been subject to liquid chromatography mass spectrometry (LC-MSn) analysis and tested on t…

0106 biological sciences0301 basic medicineSpinaciaAntioxidantHT29 cell lineCell Survivalmedicine.medical_treatmentLiquid Chromatography-Mass Spectrometry<i>Spinacia oleracea</i> L.lcsh:QR1-502antioxidant activitySpinacia oleracea L.Anti-proliferative activity; Antioxidant activity; Comet Assay; HT29 cell line; Liquid Chromatography-Mass Spectrometry; Spinacia oleracea L;medicine.disease_cause01 natural sciencesBiochemistrylcsh:MicrobiologyAntioxidantsMass SpectrometryArticle03 medical and health sciencesSpinacia oleraceamedicineHumansFood scienceMolecular BiologyCell ProliferationbiologyAbiotic stressChemistryChemistry PhysicalPlant Extractsfood and beveragesBiological activitybiology.organism_classificationAntineoplastic Agents PhytogenicComet assayFruit and Vegetable JuicesOxygen030104 developmental biologyPhytochemicalSpinachanti-proliferative activityComet AssayDrug Screening Assays AntitumorHT29 CellsOxidative stress010606 plant biology & botanyChromatography LiquidBiomolecules
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Production of extracellular fructans by Gluconobacter nephelii P1464.

2015

UNLABELLED Bacterial extracellular fructans, known as levans, have potential applications in food, pharmaceutical and cosmetic industries and high fructan producing strains could contribute into the cost reduction and more extensive commercial usage of them. An acetic acid bacteria (AAB) isolate P1464 was obtained from the Microbial Strain Collection of Institute of Microbiology and Biotechnology, University of Latvia and identified as Gluconobacter nephelii by DNA-DNA hybridization and the formation of extracellular fructans by this strain was confirmed. Isolated extracellular fructose polymers were characterized using FT-IR spectroscopy and the structural features of fructan appeared as s…

0106 biological sciences0301 basic medicineSucroseGluconobactermedicine.medical_treatment030106 microbiologyGluconobacterFructose01 natural sciencesApplied Microbiology and BiotechnologyZymomonas mobilis03 medical and health scienceschemistry.chemical_compoundFructanBioreactors010608 biotechnologySpectroscopy Fourier Transform InfraredExtracellularmedicineAcetic acid bacteriaAcetic AcidbiologyPrebioticPolysaccharides BacterialFructosebiology.organism_classificationDNA FingerprintingFructansMolecular WeightchemistryBiochemistryLetters in applied microbiology
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Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture.

2017

Thioredoxins (Trxs), key components of cellular redox regulation, act by controlling the redox status of many target proteins, and have been shown to play an essential role in cell survival and growth. The presence of a Trx system in the nucleus has received little attention in plants, and the nuclear targets of plant Trxs have not been conclusively identified. Thus, very little is known about the function of Trxs in this cellular compartment. Previously, we studied the intracellular localization of PsTrxo1 and confirmed its presence in mitochondria and, interestingly, in the nucleus under standard growth conditions. In investigating the nuclear function of PsTrxo1 we identified proliferati…

0106 biological sciences0301 basic medicineTFs transcription factorsOverexpressionBiologíaBiFC bimolecular fluorescence complementationClinical BiochemistryCell Culture TechniquesTobacco BY-2 cells01 natural sciencesBiochemistryTBY-2 tobacco bright yellow-2DTT 14-dithiothreitolBimolecular fluorescence complementationThioredoxinsGene Expression Regulation PlantTrx thioredoxinlcsh:QH301-705.5GFP green fluorescent proteinlcsh:R5-920biologyProliferating cell nuclear antigen (PCNA)Cell cycleGlutathione3. Good healthCell biologyMitochondriaNTR NADPH thioredoxin reductaseProtein TransportDEM diethyl maleateRT-qPCR Reverse transcription quantitative polymerase chain reactionThioredoxinlcsh:Medicine (General)Oxidation-ReductionAMS 4-acetamido-4-maleimidylstilbene-22-disulfonic acidResearch PaperPCNA proliferating cell nuclear antigenOex overexpressingCell cycleNucleusThioredoxin o103 medical and health sciencesROS reactive oxygen speciesDownregulation and upregulationProliferating Cell Nuclear AntigenTobaccoDAPI 46-diamidine-2-phenylindolmCBM monochlorobimaneCellular compartmentCell NucleusCell growthOrganic ChemistryBotánicaPeasMolecular biologyYFP yellow fluorescent proteinProliferating cell nuclear antigenTBS Tris-buffered salineOD optical density030104 developmental biologylcsh:Biology (General)Cell cultureRNA reactive nitrogen speciesbiology.proteinPrx peroxiredoxinBSA bovine serum albumin010606 plant biology & botanyRedox biology
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UV-screening and springtime recovery of photosynthetic capacity in leaves of Vaccinium vitis-idaea above and below the snow pack

2019

International audience; Evergreen plants in boreal biomes undergo seasonal hardening and dehardening adjusting their photosynthetic capacity and photoprotection; acclimating to seasonal changes in temperature and irradiance. Leaf epidermal ultraviolet (UV)-screening by flavonols responds to solar radiation, perceived in part through increased ultraviolet-B (UV-B) radiation, and is a candidate trait to provide cross-photoprotection. At Hyytiälä Forestry Station, central Finland, we examined whether the accumulation of flavonols was higher in leaves of Vaccinium vitis-idaea L. growing above the snowpack compared with those below the snowpack. We found that leaves exposed to colder temperature…

0106 biological sciences0301 basic medicineTime FactorsPhotoinhibitionBOREALPhysiologyPlant ScienceForests01 natural sciencesPlant EpidermisAnthocyaninsSoilFlavonolsLOW-TEMPERATURESnowPhotosynthesis1183 Plant biology microbiology virologychemistry.chemical_classificationspring dehardening.CLIMATE-CHANGEbiologyChemistryTemperatureUnderstoreyHorticultureLIGHTSeasonsVacciniumUltraviolet RaysGrowing seasonPhotosynthesisDWARF SHRUB03 medical and health sciencesLEAFPHOTOSYSTEM-IIGenetics[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyVaccinium vitis-idaeaFlavonoidsSpring dehardeningPhotoprotectionSpectral qualityPhotosystem II Protein ComplexPigments Biological15. Life on landEvergreenbiology.organism_classificationPhotosynthetic capacitySUB-ARCTIC HEATHPlant Leaves030104 developmental biology13. Climate actionPhotoprotectionWINTERB RADIATIONArctic browning010606 plant biology & botany
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Boosting Biomass Quantity and Quality by Improved Mixotrophic Culture of the Diatom Phaeodactylum tricornutum

2021

Diatoms are photoautotrophic unicellular algae and are among the most abundant, adaptable, and diverse marine phytoplankton. They are extremely interesting not only for their ecological role but also as potential feedstocks for sustainable biofuels and high-value commodities such as omega fatty acids, because of their capacity to accumulate lipids. However, the cultivation of microalgae on an industrial scale requires higher cell densities and lipid accumulation than those found in nature to make the process economically viable. One of the known ways to induce lipid accumulation in Phaeodactylum tricornutum is nitrogen deprivation, which comes at the expense of growth inhibition and lower c…

0106 biological sciences0301 basic medicine[SDV.BIO]Life Sciences [q-bio]/BiotechnologyPhotobioreactorBiomassPlant Sciencelcsh:Plant culture01 natural sciences03 medical and health sciencesAlgaemixotrophic growthgenome-scale metabolic modelSettore BIO/04 - Fisiologia Vegetalelcsh:SB1-1110Phaeodactylum tricornutumbiomass productivityOriginal ResearchbiologyChemistryPlinear programmingbiology.organism_classificationPulp and paper industryP. tricornutumdiatomLight intensity030104 developmental biologyDiatomtricornutumBiofuelmetabolismMixotroph010606 plant biology & botanyFrontiers in Plant Science
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