Search results for "HeLa"

Identification of a Dynein Interacting Domain in the Papillomavirus Minor Capsid Protein L2

2006

ABSTRACT Papillomaviruses enter cells via endocytosis (H. C. Selinka et al., Virology 299:279-287, 2002). After egress from endosomes, the minor capsid protein L2 accompanies the viral DNA to the nucleus and subsequently to the subnuclear promyelocytic leukemia protein bodies (P. M. Day et al., Proc. Natl. Acad. Sci. USA 101:14252-14257, 2004), suggesting that this protein may be involved in the intracytoplasmic transport of the viral genome. We now demonstrate that the L2 protein is able to interact with the microtubule network via the motor protein dynein. L2 protein was found attached to microtubules after uncoating of incoming human papillomavirus pseudovirions. Based on immunofluoresce…

Lead Nanowires for Microaccumulators Obtained Through Indirect Electrochemical Template Deposition

2010

Metallic lead nanowires were deposited within pores of commercial anodic alumina membranes having an average pore diameter of 210 nm. "Direct" electrodeposition was attempted from 0.1 M Pb(NO 3 ) 2 aqueous solution with a variable concentration of H 3 BO 3 as a chelating agent, but it gave unsatisfactory results. An "indirect" two-step deposition procedure was then adopted, consisting of the anodic electrodeposition of α-PbO 2 nanowires, followed by their in situ reduction to metallic lead. Both these processes occurred at a high rate so that the indirect method led to a complete template pore filling with pure polycrystalline Pb in short times and with a high current efficiency.

A nitroreductase and glutathione responsive nanoplatform for integration of gene delivery and near-infrared fluorescence imaging

2020

A novel platform rationally integrating indocyanine green analogues and an arginine-rich dendritic peptide with both nitroreductase (NTR) and glutathione (GSH) reduction responsive linkers was developed. This multifunctional platform can enable selective and efficient gene delivery and specific turn-on fluorescence imaging in tumors.

Double stimuli-responsive polysaccharide block copolymers as green macrosurfactants for near-infrared photodynamic therapy

2019

The NIR absorbing photosensitizer phthalocyanine zinc (PC(Zn)) was stabilized in aqueous media as water-dispersible nanoparticles with a reduction- and pH-responsive full polysaccharide block copolymer. A cellular uptake and also photo switchable intracellular activity of the cargo upon irradiation at wavelengths in the near infrared region were shown. The block copolymer was synthesized by applying a copper-free click strategy based on a thiol exchange reaction, creating an amphiphilic double-stimuli-responsive mixed disulfide. The dual-sensitive polysaccharide micelles represent a non-toxic and biodegradable green macrosurfactant for the delivery of phthalocyanine zinc. By encapsulation i…

Clinical and Biological Characterization of Patients with Low/Intermediate-1 Risk Myelodysplastic Syndrome and Iron Overload

2012

Abstract Abstract 4956 Introduction. Patients with Myelodysplastic Syndrome (MDS) are susceptible to developing iron overload as a response to the red blood cell (RBC) transfusions and ineffective hematopoiesis. This iron overload (IOL) is characterized by an increase in oxygen-reactive species accompanied by a decrease in antioxidants, and results in hepatic, cardiac and endocrine disorders, as well as an increased risk of infection. Ineffective hematopoiesis promotes iron absorption at intestinal level. This process is enhanced by the presence of mutations in the hereditary hemochromatosis gene (HFE). This study aims to define the features that accompany patients with iron overload, compa…

Complexes of organothallium(III) with Cis-1,2-dicyanoethylenedithiolate ion and a ‘tripod’ ligand. Synthesis, infrared and proton magnetic resonance …

1980

Abstract The reaction of cis-1,2-dicyanoethylenedithiolate ion (mnt2− with organothallium(III) derivatives has been examined. The complexes [(CH3)2Tl]2mnt and [(C4H9)4N] [(C6H5)2Tl mnt] have been synthesized. The results seem to indicate a trend in reactivity similar to that of arylmercury(II) derivatives towards chelating agents with nucleophilic character. In addition, the complex [(CH3)2Tl(tren)] [BPh4] has been obtained as a test of the complexing ability of 2,2′, 2″-triaminotriethylamine(tren) towards organometallic moieties. I.r. and 1H n.m.r. are presented and discussed.

6-Thiopurine complexes of organotin(IV) moieties. Synthesis and structural characterization by infrared and Mössbauer spectroscopy

1982

Abstract The novel complexes Me3Sn(6-TP−1) and Bun2Sn(6-TP−1)2, where 6-TP−1 is the mono-anion of 6-thiopurine, have been synthesized. The sites of stannylation of 6-TP have been inferred from the known directions of electrophilic substitution reactions of 6-TP itself. The compounds have been characterized by infrared and Mossbauer spectroscopy. A solid state polymeric structure has been proposed for Me3Sn(6-TP−1), where planar SnC3 skeletons are bridged by 6-TP−1 (thione tautomer) axially bound to Sn through N(3) and N(1) atoms. For Bun2Sn(6-TP−1)2, a molecular structure has been advanced, with the ‘aromatic’ ligand anions chelating Sn by S-N(7) atoms.

The Use of the Macrocyclic Chelator DOTA in Radiochemical Separations

2019

Deep-Red-Emitting Electrochemical Cells Based on Heteroleptic Bis-chelated Ruthenium(II) Complexes

2009

Two ruthenium(II)-based complexes were prepared that show intense deep-red light emission at room temperature. Solid-state electroluminescent devices were prepared using one of the ruthenium complexes as the only active component. These devices emit deep-red light at low voltages and exhibit extraordinary stabilities, demonstrating their potential for low-cost deep-red light sources.

Diastereospecific control in the synthesis of enantiomerically pure bis-equatorial rhodium(II) catalysts by chiral phosphanes

2000

The reaction of Rh2(O2CR)4 [R = CH3, CF3] with the chiral phosphane (1S,2S,5R)-(2-hydroxy-5-isopropenyl-2-methylcyclohexyl)(diphenyl)phosphane, (+)PPh2(CH−R*−OH) or its enantiomer (1R,2R,5S)-(2-hydroxy-5-isopropenyl-2-methylcyclohexyl)(diphenyl)phosphane, (−)-PPh2(CH−R*−OH), results in the specific formation of the products (P)-Rh2(μ-O2CR)2(η1-O2CR)2{η2-(+)PPh2(CH−R*−OH)}2 [P(+)1, R = CH3; P(+)2 R = CF3] and (M)-Rh2(μ-O2CR)2(η1-O2CR)2{η2-(−)-PPh2(CH−R*−OH)}2 [M(−)1, R = CH3; M(−)2, R = CF3] in a high yield. Their synthesis, characterisation and catalytic behaviour in metal−carbenoid reactions are reported. X-ray analysis of P(+)2 and M(−)2 shows, in each case, a Rh24+ unit supported by two …