Search results for "Heterokaryon"
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Complementation among developmental mutants in Aspergillus nidulans.
1973
In heterokaryons between pairs of aconidial mutants of Aspergillus nidulans one of the component strains usually shows a striking prevalance in the contribution to the conidial crop. By assuming that the prevailing strain is blocked earlier and the succumbent one later in the process of differentiation, a series of mutations can be arranged in a consistent order. Some mutant strains do not fit the scheme exactly but show a general tendency to be succumbent to “early” mutants and prevalent over the “late” ones. A criterion for arraying genes involved in differentiation according to the order of their physiological action is proposed.
Self-diploidization in Saccharomyces cerevisiae kar2 heterokaryons
1993
Zygotes isolated by micromanipulation from crosses of Saccharomyces cerevisiae strains, one of which carries a kar mutation, give rise most frequently to cytoductant colonies showing the nuclear constitution of either one of the two haploid parental strains. In crosses of kar2-1 strains to wild-type, about 10% of the cytoductants of both mating types are homozygous autodiploids. There is evidence indicating that self-diploidization occurs by fusion between sibling nuclei in the heterokaryotic zygote. Here we describe this phenomenon and propose to take advantage of it for the construction of genotypically-defined diploids able to mate, and of polyploid strains, which are useful tools in gen…