Search results for "Hybridization"
showing 10 items of 812 documents
Performance of interdigitated nanoelectrodes for electrochemical DNA biosensor.
2003
An electrochemical methodology for bio-molecule sensing using an array of well-defined nanostructures is presented. We describe the fabrication by e-beam lithography of nanoelectrodes consisting of a 100 micro m x 50 micro m area containing interdigitated electrodes of 100 nm in width and interelectrode distance of 200 nm. Sensitivity and response time of the nanoelectrodes are compared to the responses of macro- and microelectrodes. The specificity of the sensor is studied by modifying the gold electrodes with DNA. The technique enables to characterize both single and double-stranded DNA of 15 nucleotides. A special electrochemical cell is adapted to control the temperature and measure the…
Interspecific hybridization improves the performance of Lotus spp. under saline stress
2019
Abstract Salinity is one of the most frequent limiting conditions in pasture production for grazing livestock. Legumes, such as Lotus spp. with high forage quality and capable of adapting to different environments, improves pasture performance in restrictive areas. In order to determine potential cultivars with better forage traits, the current study assess the response to salt stress of L. tenuis, L. corniculatus and a novel L. tenuis x L. corniculatus accession. For this purpose, chlorophyll fluorescence, biomass production, ion accumulation and anthocyanins and proanthocyanidins levels have been evaluated in control and salt-treated plants PSII activity was affected by salt in L. tenuis,…
Investigations for fine mapping of amplifications in chromosome 3q26.3-28 frequently occurring in squamous cell carcinomas of the head and neck.
2002
<i>Objective:</i> Overrepresentations of chromosomal material on the long arm of chromosome 3 frequently occur in squamous cell carcinoma of the head and neck. This experimental study was conducted for further fine mapping of these overrepresentations by interphase fluorescence in situ hybridization (FISH) of tumor cells in cell lines. <i>Methods:</i> Seven cell lines derived from squamous cell carcinomas of the head and neck were investigated by comparative genomic hybridization to analyze unbalanced chromosomal aberrations. Overrepresentations of chromosomal material on the telomeric part of the long arm of chromsome 3 were further analyzed by interphase FISH using…
Eight million years of maintained heterozygosity in chromosome homologs of cercopithecine monkeys
2020
In the Cercopithecini ancestor two chromosomes, homologous to human chromosomes 20 and 21, fused to form the Cercopithecini specific 20/21 association. In some individuals from the genus Cercopithecus, this association was shown to be polymorphic for the position of the centromere, suggesting centromere repositioning events. We set out to test this hypothesis by defining the evolutionary history of the 20/21 association in four Cercopithecini species from three different genera. The marker order of the various 20/21 associations was established using molecular cytogenetic techniques, including an array of more than 100 BACs. We discovered that five different forms of the 20/21 association w…
LRRK2 is expressed in areas affected by Parkinson's disease in the adult mouse brain.
2006
The leucine-rich repeat kinase 2 (LRRK2) gene was recently found to have multiple mutations that are causative for autosomal dominant inherited Parkinson's disease (PD). Previously, we used Northern blot analysis to show that this gene was expressed in the cerebellum, cerebral cortex, medulla, spinal cord, occipital pole, frontal lobe, temporal lobe and caudate putamen. However, a more comprehensive map of LRRK2 mRNA localization in the central nervous system is still lacking. In this study we have mapped the distribution of the mRNA encoding for LRRK2 using nonradioactive in situ hybridization. We detected a moderate expression of this PD-related gene throughout the adult B2B6 mouse brain.…
Interstitial deletions of chromosome 1p: novel 1p31.3p22.2 microdeletion in a newborn with craniosynostosis, coloboma and cleft palate, and review of…
2022
Abstract Background Rearrangements of unstable DNA sequences may alter the structural integrity or the copy number of dose-sensitive genes, resulting in copy number variations. They may lead more frequently to deletions, in addition to duplications and/or inversions, which are the underlying pathogenic mechanism of a group of conditions known as genomic disorders (or also contiguous gene syndromes). Interstitial deletions of the short arm of chromosome 1 are rare, and only about 30 patients have been reported. Their clinical features are variable, in respect of the extent of the deleted region. They include global developmental delay, central nervous system (CNS) malformations, craniosynost…
Clostridium ģints baktēriju noteikšana dzeramajā ūdenī izmantojot Fluorescento in situ hibridizāciju
2015
Bakalaura darba mērķis bija ātri un precīzi identificēt dzeramajā ūdenī Clostridium ģints baktērijas izmantojot Fluorescento in situ hibridizāciju. Līdz ar to tika izmērīts un salīdzināts fluorescences intensitātes līmenis dažādām Clostridium sugām, kā arī citiem mikroorganismiem izmantojot Clostridium spp. specifiskas fluorescentās zondes. 92% no Clostridium acetobutylicum izmērītam šūnām, relatīvās gaismas vienības (RLU) pārsniedza 500 vienību robežu, 99% no Clostridium beijernickii un 96% no Clostridium tetahomorphum, salīdzinājumā ar Sphingomonas paucimobilis, kur tikai 2% pārsniedza 300 RLU. Veiktais variācijas analīzes tests (ANOVA) liecināja, ka starp Clostridium ģints baktērijām un …
Inheritance patterns of photoperiodic diapause induction in Leptinotarsa decemlineata
2016
Photoperiod is a reliable indicator of season and an important cue that many insects use for phenological synchronization. Undergoing range expansion insects can face a change in the local photoperiod to which they need to resynchronize. Rapid range expansion can be associated with rapid photoperiodic adaptation, which can be associated with intense selection on strongly heritable polygenic traits. Alternatively, it is proposed that, in insects with an XO sex-determination system, genes with large effect residing on the sex chromosome could drive photoperiodic adaptation because the gene or genes are exposed to selection in the sex carrying only a single X-chromosome. The present study seek…
DNA-DNA hybridization and ribotyping of Acidovorax delafieldii isolates from eels and aquatic environments
1992
Eleven strains of Acidovorax isolated from a freshwater lake and from healthy and diseased European eels in a hatchery were investigated by genotypic methods. DNA-DNA slot blot hybridization confirmed the identity of all strains as A. delafieldii. Isolates were heterogeneous in EcoR1 ribopatterns with four types recognizable. Most freshwater and healthy eel strains had the same ribotype but differed from the disease-associated strains. Typical ribopatterns of A. delafieldii contained 1·3, 1·5, and 3·2 kb fragments which provided useful additional taxonomic markers for species identification.
Copy number variation and missense mutations of the agouti signaling protein (ASIP) gene in goat breeds with different coat colors.
2009
In goats, classical genetic studies reported a large number of alleles at the Agouti locus with effects on coat color and pattern distribution. From these early studies, the dominant A(Wt) (white/tan) allele was suggested to cause the white color of the Saanen breed. Here, we sequenced the coding region of the goat ASIP gene in 6 goat breeds (Girgentana, Maltese, Derivata di Siria, Murciano-Granadina, Camosciata delle Alpi, and Saanen), with different coat colors and patterns. Five single nucleotide polymorphisms (SNPs) were identified, 3 of which caused missense mutations in conserved positions of the cysteine-rich carboxy-terminal domain of the protein (p.Ala96Gly, p.Cys126Gly, and p.Val1…