Search results for "Hybridization"
showing 10 items of 812 documents
In situ analysis of the bacterial communities associated to farmed eel by whole-cell hybridization.
1999
Bacterial communities in water samples and eel slime were investigated by fluorescence in situ hybridization of whole bacterial cells in an eel intensive culture system over 1 year. A newly developed probe, matching 27 Vibrio spp., and a specific probe for Vibrio vulnificus were used. Phylogenetic probes complementary to selected regions of the 16S and 23S ribosomal RNA revealed that Proteobacteria of the alpha and beta subclass were predominant in water and eel slime. Members of the gamma subclass (e.g. vibrios and aeromonads) were more abundant in eel slime, although no V. vulnificus was detected.
Detection of endophytic bacteria in leaves of Vitis vinifera by using fluorescence in situ hybridization
2008
Previous investigation on five cultivars of healthy Sicilian grapevine allowed the isolation of endophytic bacteria belonging to Bacillus genus from different organs (bud, leaf, stalk and shoot). The aim of this work was to use fluorescence in situ hybridization (FISH) experiments in healthy and damaged leaf tissues of Vitis vinifera to visualize and localize bacteria associated with plant materials. The leaves were cleared to minimize the autofluorescence of the plant fragments. The use of fluorescently labelled bacterial probe Eub338 in FISH experiments on discoloured grapevine leaf disks allowed the estimation of the spatial distribution of different bacterial colonies. At the same time,…
Experimental introgression in Drosophila : Asymmetric postzygotic isolation associated with chromosomal inversions and an incompatibility locus on th…
2022
Interspecific gene flow (introgression) is an important source of new genetic variation, but selection against it can reinforce reproductive barriers between interbreeding species. We used an experimental approach to trace the role of chromosomal inversions and incompatibility genes in preventing introgression between two partly sympatric Drosophila virilis group species, D. flavomontana and D. montana. We backcrossed F1 hybrid females from a cross between D. flavomontana female and D. montana male with the males of the parental species for two generations and sequenced pools of parental strains and their reciprocal 2nd generation backcross (BC2mon and BC2fla) females. Contrasting the obser…
WILLIAMS-BEUREN MAPPING IN CALLITHRIX ARGENTATA, CALLICEBUS CUPREUS AND ALOUATTA CARAYA INDICATES DIFFERENT PATTERNS OF CHROMOSOMAL REARRANGEMENTS IN…
2007
Human chromosome 7 has a complex syntenic origin. It was divided into two segments in both the ancestral primate karyotype and in Platyrrhini. Apparently, a small segment in the ancestral platyrrhine karyotype was associated with HSA5 and the remainder formed a middle-sized submetacentric. We tested the dynamics of platyrrhine chromosomes by hybridizing the locus specific Willams-Beuren probe (7q 11.23, 450 kb) to chromosomes of representative species from the three families of the New World monkeys recently proposed by molecular genomics: Cebidae, Callithrix argentata (bare ear marmoset or silvery marmoset, 2n = 44); Pitheciidae, Callicebus cupreus [red titi monkey, or coppery monkey, 2n =…
The extent and causes of interspecific reproductive interactions in damselflies
2015
Molecular Cytogenetics as a Tool for Primate Taxonomic identification: A Case Study in Captivity
2015
caratteristiche peculiari essendo notturne e monogame. Hanno un’ampia distribuzione geografica che si estende dallo stretto di Panama al nord dell’Argentina; questo determina una notevole complessità nella distribuzione e di conseguenza nell’interpretazione sistematica e filogenetica. Lo studio del genere Aotus mediante l’analisi di caratte ri morfologici e genetici ha prodotto una tassonomia controversa. Inizialmente era riconosciuta solo la specie Aotus trivirgatus, successivamente in base alla colorazione del pelo del collo, alla diversa suscettibilità alla malaria e ai dati citogenetici sono state identificate fino a nove specie, oltre a due sibling species, per un totale di 11.1,2 Il n…
Imperfections et hybridation générique dans « Les Employés » d’Honoré de Balzac
2020
L’imperfezione dell’essere umano e delle sue costruzioni sociali è al centro della Comédie humaine. Il presente articolo intende esplorare questo aspetto della produzione letteraria di Balzac attraverso l’analisi de Les Employés (1845), in cui la mediocrità del mondo burocratico viene esaminata in dettaglio. Nel romanzo, la mediocrità vince sul merito, ma la rappresentazione dell’imperfezione trascende la dimensione puramente narrativa: Les Employés è un grande laboratorio in cui l’ibridazione tra genere romanzesco e genere teatrale interroga i limiti della creazione letteraria. The imperfection of the human being and his social constructions is at the heart of the Comédie humaine. This art…
Järjestödemokratia ja ammatillinen johtaminen suomalaisten sosiaali- ja terveysjärjestöjen myytteinä ja seremonioina
2023
Yhdistyslaissa korostuu toiminnan jäsenvaltaisuus. Toimintaympäristön muutos on kuitenkin johtanut ammatillistumiseen, ja palkattujen toimihenkilöiden asema on korostunut. Artikkelissa pohditaan, onko jäsendemokratiasta muodostumassa ammatillistuvissa järjestöissä myytti, jota pidetään yllä seremoniallisesti, kun järjestöjä käytännössä johtavat palkatut ammattilaiset. The article examines the role of social and health associations as civil society organisations (CSOs) in Finnish society. In recent decades the main sources of public funding of social and health associations in Finland have called for a stronger managerial and professional orientation, with a welfare policy guidance focus. CS…
Language for International communication: Linking Interdisciplinary Perspectives on Cultural, Professional and Scientific Capacity Building: Book of …
2019
Accounting, microfoundations, hybridization and longitudinal conflict in a Finnish health care organization
2021
PurposeThis article analyzes the cognitive microfoundations, conflicting institutional logics and professional hybridization in a case characterized by conflict.Design/methodology/approachIn contrast to the majority of earlier studies focusing on special health care, the study was conducted in a Finnish basic health care organization. The empirical data include 36 interviews, accounting reports, budgets, newspaper articles and meeting notes collected 2013–2018.FindingsThe use of accounting techniques in this case did not offer professionals sufficient support under conditions of conflict. The authors suggest that this perceived lack of support intensified the negative emotions toward accoun…