Search results for "Hydrolysate"
showing 10 items of 46 documents
Evaluation of in vitro iron bioavailability in free form and as whey peptide-iron complexes
2018
Abstract Finding alternatives for food fortification in a bioavailable form of iron is needed because iron deficiency leads to several diseases. Iron solubility and in vitro iron absorption were evaluated in free and complexed forms, as iron salts or peptide-iron complexes. Whey peptide-iron complexes were synthesized with various ligands (whey protein hydrolysate; its fractions >5 kDa and 85%), only complexes that were synthesized with low-molecular-mass peptides (
Preliminary characterization of the material released to the culture medium by Candida albicans yeast and mycelial cells.
1995
Culture filtrate concentrates were obtained from Candida albicans yeast and mycelial cells grown in the presence of 14C-protein hydrolysate for radioactive labeling of cellular polypeptides. Both growth forms released to the medium minor but significant amounts of proteinaceous materials. The analysis of culture filtrate concentrates by means of SDS-polyacrylamide gel electrophoresis and fluorography revealed a similar and complex electrophoretic pattern, though some qualitative and quantitative differences between samples obtained from yeast and mycelial cells were observed. Materials released, mostly composed of mannoproteins as shown by their affinity towards concanavalin A, presented (i…
Factors Influencing Digitalis Obscura Mesophyll Protoplast Development
1988
In a previous work (1), we found that a combination of liquid-over-agar-solidified MS (2) modified medium was superior to either all liquid or all solid medium for culture of Digitalis obscura L. mesophyll protoplasts. In order to achieve optimum culture conditions we now report on the influence of a different gelling agent (agarose) as well as the effect of several nutrient media on D. obscura mesophyll protoplasts development.
Dialyzability of iron, zinc, and copper of different types of infant formulas marketed in Spain
1998
The bioavailability of trace elements in infant formulas is affected by different physiological and dietetic factors. In vitro methods based on element dialyzability have been proposed to estimate the bioavailability. Infant formulas of the same type but from different manufacturers can differ in the salt used for supplementation and in the contents of other components that can affect mineral bioavailability. The aim of our study is to estimate the dialyzability of iron, zinc, and copper of formulas marketed in Spain, in order to detect possible differences in formulas of the same type coming from different manufacturers. At the same time, the effects of the type of formula, the composition…
Calcium dialysability as an estimation of bioavailability in human milk, cow milk and infant formulas
1999
Abstract The calcium dialysability of human milk, cow milk and infant formulas is estimated by applying the in vitro method (Miller, Schricker, Rasmussen, & Van Campen, (1981). American Journal of Clinical Nutrition, 34, 2248–2256) slightly modified, as a measure of its bioavailability. The influence of the protein composition of infant formula samples on calcium bioavailability is also studied. Whole cow milk, a pool of human milk and 18 commercial infant formulas of 6 different types are analysed. The highest values of calcium dialysability (mg Ca/100 ml infant formula) correspond to premature (13.6) and follow-up formulas (11.8) and the lowest ones to adapted formulas (3.7); the latter v…
Antihypertensive effect of a bovine lactoferrin pepsin hydrolysate: Identification of novel active peptides
2012
et al.
An antihypertensive lactoferrin hydrolysate inhibits angiotensin I-converting enzyme, modifies expression of hypertension-related genes and enhances …
2015
This study was aimed to explore whether an antihypertensive lactoferrin hydrolysate (LFH) can inhibit angiotensin I-converting enzyme (ACE) activity and modify the expression of genes related to hypertension in human umbilical vein endothelial cells (HUVEC). LFH induced significant inhibition of ACE activity but it did not affect ACE mRNA levels after 24 h of exposure. LFH treatment significantly affected the expression of genes encoding for proteins involved in nitric oxide pathway such as soluble guanylate cyclase 1 α3 subunit (GUCY1A3; 4.42-fold increase) and nitric oxide synthase trafficking (NOSTRIN; 2.45-fold decrease). Furthermore, expression of the PTGS2/COX-2 gene encoding prostagl…
PURIFICATION OF HARDWOOD-DERIVED AUTOHYDROLYSATES
2012
Carbohydrate-containing hydrolysates (1.1 to 14.9% of wood dry matter) obtained from autohydrolysis (at 130 to 150°C for 30 to 120 minutes) of birch (Betula pendula) chips prior to pulping were purified with respect to non-carbohydrate materials, without carbohydrate losses, either by ethyl acetate extraction or XAD-4 resin treatment. In the former case, about 50% of lignin and practically all the furanoic compounds (2-furaldehyde and 5-(hydroxymethyl)furfural) could be removed, whereas in the latter case, the corresponding amounts were about 30% and 50 to 90%, respectively. A partial recovery of various unsaturated impurities is of importance, because they may act as inhibitors when bioche…
Multivariate Correlation between Analysis Data on Dissolved Organic Material from Scots Pine (Pinus sylvestris) Chips and their Autohydrolysis Pre-Tr…
2013
Various chemometric techniques were used to establish the relationship between the autohydrolysis conditions prior to pulping and the chemical compositions of the soluble organic materials removed from Scots pine (Pinus sylvestris) wood chips. The aqueous chip pre-treatments (autohydrolysis) were administered at 130 °C and 150 °C for 30, 60, 90, and 120 min, and the hydrolysates obtained were characterized in terms of total carbohydrates (various mono-, oligo-, and polysaccharides together with uronic acid side groups), volatile acids (acetic and formic acids), lignin, and furans (furfural and 5-(hydroxymethyl)furfural). Based on the analytical data gathered, a relatively accurate model for…
Two new biologically active triterpenoidal saponins acylated with salicylic acid from Albizia adianthifolia
2003
International audience; Two new oleanane-type triterpene saponins, adianthifoliosides A (1) and B (2), were isolated from a 95% ethanolic extract of roots of Albizia adianthifolia. Their structures were elucidated mainly by using a combination of 600 MHz 1D and 2D NMR techniques (COSY, NOESY, TOCSY, HSQC, and HMBC) and by FABMS and HRESIMS. Compounds 1 and 2 were characterized as glycosides of acacic acid acylated by an o-hydroxybenzoyl unit. The crude saponin mixture (CSM), compounds 1 and 2 together with 3 and 4 (prosapogenins obtained from the mild alkaline hydrolysate of the CSM), were evaluated for immunomodulatory activity on the Jurkat T cell line and for hemolytic property against s…