Search results for "Immunomagnetic Separation"

showing 5 items of 15 documents

Specific stabilization of the 4F7 molecule on dendritic cells by contact allergens.

1996

Our laboratory has recently developed the monoclonal antibody 4F7 which recognizes a molecule on dendritic cells in the dermis of mice that is upregulated after application of contact allergens in vivo. Furthermore, this antibody detects an antigen on dendritic cells in spleen, lymph nodes and colon. In order to study the influence of contact allergens on the surface expression of the 4F7 molecules on dendritic cells, FACScan analysis of splenic dendritic cells was carried out after in vitro application of contact allergens. Freshly isolated splenic dendritic cells were found to be positive for 4F7, 33D1, N418 (CD11c) and MHC class II. After overnight culture the expression of the dendritic…

MaleLangerhans cellCD11cDown-RegulationDermatologyCycloheximideAntigen-Antibody Reactionschemistry.chemical_compoundMiceAntigenmedicineAnimalsCycloheximideMonensinCells CulturedProtein Synthesis InhibitorsMHC class IIMice Inbred BALB CbiologyIonophoresImmunomagnetic SeparationAntibodies MonoclonalGeneral MedicineDendritic cellDendritic CellsAllergensMolecular biologyIn vitromedicine.anatomical_structurechemistryBiochemistryCell cultureAntigens Surfacebiology.proteinFemaleSpleenArchives of dermatological research
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Double-stranded RNA polyinosinic-polycytidylic acid immobilized onto gamma-Fe2O3 nanoparticles by using a multifunctional polymeric linker.

2007

Materials scienceMacromolecular SubstancesPolymersSurface PropertiesMolecular ConformationNanoparticleMaterials testingFerric CompoundsBiomaterialschemistry.chemical_compoundCoated Materials BiocompatibleMaterials TestingNanotechnologyGeneral Materials ScienceParticle SizeRNA Double-Strandedchemistry.chemical_classificationFerric CompoundsImmunomagnetic SeparationRNAGeneral ChemistryDouble stranded rnaPolymerCombinatorial chemistryNanostructuresPoly I-CchemistryBiochemistryPolyinosinic:polycytidylic acidCrystallizationLinkerBiotechnologySmall (Weinheim an der Bergstrasse, Germany)
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Large scale preparation of human MHC class II+ integrin beta(1)+ Tregs.

2010

Abstract The human CD4 + CD25 + FoxP3 + regulatory T cell population (Tregs) contains both MHC class II + and MHC class II − cells. MHC class II + Tregs belong to the integrin α 4 β 1 + subpopulation and exclusively execute contact-dependent suppressive activity. Here we present a method optimized for isolation of these MHC class II expressing Tregs from large leukaphereses products using magnetic microbeads that achieves a reproducible purity of more than 90% and enables the use of this small-sized Treg population in pre-clinical application and basic research.

Regulatory T cellImmunologyPopulationIntegrinchemical and pharmacologic phenomenaIntegrin alpha4beta1T-Lymphocytes RegulatoryT-Lymphocyte SubsetsmedicineImmune ToleranceImmunology and AllergyHumansIL-2 receptorLeukapheresiseducationCells CulturedMHC class IIeducation.field_of_studybiologyImmunomagnetic SeparationHistocompatibility Antigens Class IIInterleukin-2 Receptor alpha SubunitFOXP3hemic and immune systemsForkhead Transcription FactorsT lymphocyteMHC restrictionFlow CytometryCell biologyHigh-Throughput Screening Assaysmedicine.anatomical_structureImmunologyCD4 Antigensbiology.proteinJournal of immunological methods
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Superparamagnetic γ-Fe2O3 nanoparticles with tailored functionality for protein separation

2007

Polymer coated superparamagnetic gamma-Fe(2)O(3) nanoparticles were derivatized with a synthetic double-stranded RNA [poly(IC)], a known allosteric activator of the latent (2-5)A synthetase, to separate a single 35 kDa protein from a crude extract which cross reacted with antibodies raised against the sponge enzyme.

Surface PropertiesAllosteric regulationNanoparticleLigandsFerric CompoundsCatalysisMagneticsProtein purification2'5'-Oligoadenylate SynthetaseMaterials ChemistryAnimalsParticle Sizechemistry.chemical_classificationBinding SitesMolecular StructurebiologyImmunomagnetic SeparationMetals and AlloysRNADNA-Directed RNA PolymerasesGeneral ChemistryPolymerbiology.organism_classificationPoriferaSurfaces Coatings and FilmsElectronic Optical and Magnetic MaterialsSpongeEnzymeBiochemistrychemistryCeramics and CompositesNanoparticlesPeptidesSuperparamagnetismChemical Communications
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Oxymetazoline modulates proinflammatory cytokines and the T‐cell stimulatory capacity of dendritic cells

2007

The nasal decongestant oxymetazoline (OMZ) is frequently used in the topical treatment of rhinitis/sinusitis. As proinflammatory cytokines play a critical role in the development and maintenance of local inflammation, the aim of this study was to investigate the influence of OMZ on immune cells in order to diminish the mucosal infiltration of the nose. Peripheral blood mononuclear cells (PBMC) from buffy coats of healthy volunteers were isolated and stimulated in the presence or absence of OMZ. In addition, monocyte-derived dendritic cells (DC) were generated and different concentrations of OMZ were added. DC phenotype and their T-cell stimulatory properties were analysed. The vasoactive su…

oxymetazolinemedicine.medical_treatmentT cellT-LymphocytesInflammationEnzyme-Linked Immunosorbent AssayDermatologyimmunomodulationLymphocyte ActivationBiochemistryProinflammatory cytokinerhinitismedicineHumansAntigen-presenting cellMolecular BiologyCells CulturedDose-Response Relationship Drugbusiness.industryImmunomagnetic SeparationDendritic cellDendritic CellsFlow CytometryNasal decongestantNasal DecongestantsCytokinemedicine.anatomical_structureImmunologyproinflammatory cytokinesLeukocytes MononuclearCytokinesTumor necrosis factor alphaOriginal Articlemedicine.symptombusinessExperimental Dermatology
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