Search results for "Inoculation"
showing 10 items of 105 documents
Can the grey mould disease of the grape-vine be controlled by yeast?
2000
Botrytis cinerea has been found to be highly pathogenic to ‘Chardonnay’ and ‘Pinot noir’ cultivars of the grape-vine producing the characteristic grey mould symptoms within 7 days of inoculation to the vitro-plants. The yeast Pichia anomala (strain FY-102), isolated from apple skin, was found to be antagonistic to B. cinerea as it completely inhibited the appearance of the grey mould symptoms when grown together. The yeast was responsible for morphological changes such as coagulation and leakage of the cytoplasm of B. cinerea. The pathogen, when applied together with P. anomala, failed to bring about the grey mould symptoms on the grape-vine, suggesting that the yeast could control the expr…
Countering accusations with inoculation: The moderating role of consumer-company identification.
2013
Abstract Accusations of wrongdoing, baseless or justified, can severely tarnish a company's reputation. Once disseminated, even baseless accusations can persist and cause considerable damage for a company. This study examines the proactive crisis communication strategy of inoculating individuals against invalid accusations before they go viral. An experiment was conducted in a real world consumer context among members of an online consumer panel using an electronics discounter as the research stimulus. Expanding previous inoculation research on the role of value-relevant involvement for inoculation and the effectiveness of inoculation in the case of different preexisting attitudes, we find …
Influence of the methodology of inoculation in the performance of air-breathing microbial fuel cells
2017
In this work, four air-breathing microbial fuel cells (AB-MFC) were operated for 1 month in order to determine if the methodology of inoculation affects the steady-state performance of this type of MFCs. For this purpose, anaerobic and aerobic sludge were fed to two identical AB-MFCs without any external carbon source into a tight sealed environment during the first three days of start-up. For comparison purposes, other two AB-MFCs were operated mixing the initial sludge and an amount of sodium acetate as substrate. Results point out that the inoculation procedure does not affect the steady-state treatment capacity of the cells but it affects very seriously the production of electricity. On…
Influence of the initial sludge characteristics and acclimation on the longterm performance of double-compartment acetate-fed microbial fuel cells
2018
Abstract In this work, three double-compartment MFCs (DC-MFC) were operated for 1 month in order to compare their performances in terms of wastewater treatment capacity and electricity production and to get information about how this performance is influenced by the start-up procedure. To do this, they underwent different start-up procedures. One of them (aerobic-starved MFC) was inoculated with 100% fresh aerobic sludge, another (anaerobic-starved MFC) using 100% fresh anaerobic sludge, and finally a third one (aerobic-fed MFC) was inoculated using a mixture 10% fresh aerobic sludge and 90% synthetic wastewater (based on acetate). Then, from this start-up, the cells were operated exactly u…
Effects of Citrus Exocortis Viroid Infection on the Peroxidase/IAA-Oxidase System of Gynura aurantiaca and Lycoperszcon esculentum
1987
Summary The peroxidase/indoleacetic acid (IAA) oxidase system of plants of Gynura aurantiaca and Lycopersicon esculentum healthy and infected by citrus exocortis viroid (CEV) was studied. In these hosts, the infection induced an increase of both enzymic activities, when they were measured between 40 and 50 d post inoculation. The analysis of isozyme composition by polyacrylamide gel electrophoresis and specific staining of peroxidase revealed an intensification of most of the zymogram bands as well as the premature appearance of one of the isozymes in the two plants investigated. All the tomato isoperoxidases exhibited IAA-oxidase activity when measured with the oxygen electrode. These resu…
Co-Infections by Fusarium circinatum and Phytophthora spp. on Pinus radiata: Complex Phenotypic and Molecular Interactions
2021
13 Pág. Instituto de Ciencias Forestales (ICIFOR)
Impact of inoculation with the phytostimulatory PGPR Azospirillum lipoferum CRT1 on the genetic structure of the rhizobacterial community of field-gr…
2009
International audience; The phytostimulatory PGPR Azospirillum lipoferum CRT1 was inoculated to maize seeds and the impact on the genetic structure of the rhizobacterial community in the field was determined during maize growth by Automated Ribosomal Intergenic Spacer Analysis (ARISA) of rhizosphere DNA extracts. ARISA fingerprints could differ from one plant to the next as well as from one sampling to the next. Inoculation with strain CRT1 enhanced plant-to-plant variability of the ARISA fingerprints and caused a statistically significant shift in the composition of the indigenous rhizobacterial community at the first two samplings. This is the first study on the ecological impact of Azosp…
Animal Model for Cutaneous Leishmaniasis
2012
Using cutaneous leishmaniasis of mice, the existence of so-called T helper (Th) cells type 1 and type 2 had been identified more than 20 years ago. Nowadays, it is well accepted that additional T cell populations as well as B cell-mediated immunity is required for immunity against Leishmania major. Finally, using inbred mouse strains, the relevance of genetical factors that influence anti-pathogen immunity as well as elements of the skin-immune system have been identified. This protocol describes a model for murine experimental leishmaniasis that tries to mimic natural parasite transmission by several means: (1) utilization of only infectious-stage parasites that are found in sand fly saliv…
Protein and DNA fingerprinting of a soil bacterial community inoculated into three different sterile soils
2007
The functional and genetic structures of a soil bacterial community were characterized after inoculation into three different sterile soils using a protein and DNA fingerprinting method, respectively. Principal component analysis (PCA) of profiles revealed that, depending on soil characteristics, bacterial communities with similar genetic structures harbored different functional structures and thus could potentially be of differing ecological significance for soil functioning. Co-inertia analysis between protein fingerprinting data and the corresponding sets of soil physicochemical characteristics demonstrated the correlation between the functional structure of the bacterial community and s…
Response of cybrids and a somatic hybrid of lemon to Phoma tracheiphila infections
2000
The reaction of lemon hybrids obtained by symmetrical and asymmetrical protoplast fusion, toward “mal secco” infection caused by Phoma tracheiphila (Petri) Kanc. et Ghik. was examined. Resistance was tested in `Valencia' sweet orange [Citrus sinensis (L.) Osbeck] and `Femminello' lemon [C. limon (L.) Burm. f.] somatic hybrid and cybrids, by stem and leaf inoculation tests and by analysis of propagule number of P. tracheiphila in the xylem of stem-inoculated plants. In general, the somatic hybrid and the cybrids showed an intermediate degree of resistance, with slight differences in disease symptoms, in comparison with resistant `Monachello' lemon and susceptible `Femminello' lemon, used as …