Search results for "Ion microscopy"
showing 10 items of 91 documents
Two-photon exchange correction in elastic unpolarized electron-proton scattering at small momentum transfer
2015
We evaluate the two-photon exchange (TPE) correction to the unpolarized elastic electron-proton scattering at small momentum transfer $ Q^2 $. We account for the inelastic intermediate states approximating the double virtual Compton scattering by the unpolarized forward virtual Compton scattering. The unpolarized proton structure functions are used as input for the numerical evaluation of the inelastic contribution. Our calculation reproduces the leading terms in the $ Q^2 $ expansion of the TPE correction and goes beyond this approximation by keeping the full $ Q^2 $ dependence of the proton structure functions. In the range of small momentum transfer, our result is in good agreement with …
Two-photon spectroscopy of mercury and velocity-selective double resonances
2009
Two-photon laser spectroscopy of the $6\text{ }{^{1}S}_{0}\ensuremath{-}7\text{ }{^{1}S}_{0}$ transition in mercury has been performed using two copropagating continuous-wave laser beams. One laser beam is at 254 nm wavelength and can be tuned to the $6\text{ }{^{1}S}_{0}\ensuremath{-}6\text{ }{^{3}P}_{1}$ resonance. The other laser beam is at 408 nm. Two very different regimes can be distinguished, one far off resonance and one near resonance with the one-photon resonance. A resonance which is not Doppler broadened has been observed for low Rabi frequencies. This velocity-selective double resonance in a three-level ladder system is analogous to the dark resonance in three-level $\ensuremat…
Operation conditions and stability of a degenerate two-photon laser
1993
Abstract The presence of a far off-resonant intermediate level in a degenerate three-level two-photon laser model manifests through a steady pump-dependent and a dynamic frequency shift on the laser equations. The steady shift strongly affects the pump threshold necessary for emission, preventing it in certain cases. The dynamic shift makes the frequency pulling/pushing to be intensity-dependent and substantially modify the domains of existence and stability of the lasing solution permitting stable operation for large cavity losses in certain cases.
Quasi-spherical focal spot in two-photon scanning microscopy by three-ring apodization
2005
International audience; We present a beam-shaping technique for two-photon excitation (TPE) fluorescence microscopy. We show that by inserting a properly designed three-ring pupil filter in the illumination beam of the microscope, the effective optical sectioning capacity of such a system improves so that the point spread function gets a quasi-spherical shape. Such an improvement, which allows the acquisition of 3D images with isotropic quality, is obtained at the expense of only a small increase of the overall energy in the axial sidelobes. The performance of this technique is illustrated with a scanning TPE microscopy experiment in which the image of small beads is obtained. We demonstrat…
Sidelobe decline in single-photon 4Pi microscopy by Toraldo rings.
2003
We demonstrate theoretically the feasibility of single-photon 4Pi-confocal microscopy. By inserting a pair of properly designed multi-ring phase-only pupil filters in the illumination path of a 4Pi microscope the height of the sidelobes of the point spread function substantially reduced, so that there is no ambiguity in the 3D image. Then, an axial resolution up to four times higher than that of single-photon confocal microscope can be effectively achieved.
Light Sheet Fluorescence Microscopy (LSFM) for Two-Photon Excitation Imaging of Thick Samples.
2015
Over the last decades, fluorescence microscopy techniques have been developed in order to provide a deeper, faster and higher resolution imaging of three-dimensional biological samples. Within this framework, Light Sheet Fluorescence Microscopy (LSFM) became an increasingly useful and popular imaging technique able to answer several biological questions in the field of developmental biology [1]. Thanks to the spatial confinement of the excitation process within a thin sheet in the focal plane, it provides an intrinsic optical sectioning and a reduced phototoxicity. On the other side, Two-Photon Excitation (2PE), thanks to the use of IR wavelengths, has become an invaluable tool to improve i…
Morphological patterns of circumpulpal dentin affected by radiation-related caries
2020
Background The aim of this work was to evaluate the microscopic characteristics through polarized light microscopy, scanning electron microscopy and the mineral content of circumpulpal dentin of irradiated (IT) and non-irradiated teeth (NIT), with deep caries that reached the root canal. Material and methods A total of 25 IT were analyzed macroscopically, and radiographed. 5 NIT were used as controls. Two 100-μm-thick sections, per specimen, were evaluated in a polarized light microscopy and a scanning electron microscope. Demographics and clinical data were collected. Results The results did not demonstrate distinct morphology of the IT compared with NIT. Mineral content values by weight p…
Photoemission microscopy with microspot-XPS by use of undulator radiation and a high-throughput multilayer monochromator at BESSY
1999
We present a new experiment for photoelectron microspectroscopy by use of undulator radiation, which has been set up at the beamline U2 at the Berlin electron storage ring BESSY 1. This approach employs a non-imaging simulated hemispherical electron energy analyser attached to an imaging photoemission electron microscope (FOCUS IS-PEEM) with integrated microarea selector. The photoemission microscope exhibits a lateral resolution of 25 nm (with 4.9 eV UV-excitation), while the resolution with incident synchrotron radiation in the soft X-ray range is about 100-120 nm (mainly due to chromatic aberrations). Photoemission microscopy as well as photoelectron microspectroscopy of selected areas o…
Closed form for two-photon free–free transition matrix elements
2000
Abstract Two-photon free–free transitions happen in the multiphoton ionization with more than one excess photon and in bremsstralung. Up to now, the configuration space free–free transition amplitudes have not been written in closed form. We propose a modified Coulomb Green’s function (CGF) Sturmian expansion which allows one to obtain expressions for two-photon radial transition matrix elements in the closed form which are easy to continue analytically to calculate free–free transitions in H.
Preferential Type II Muscle Fiber Damage From Plyometric Exercise
2012
Context: Plyometric training has been successfully used in different sporting contexts. Studies that investigated the effect of plyometric training on muscle morphology are limited, and results are controversial with regard to which muscle fiber type is mainly affected. Objective: To analyze the skeletal muscle structural and ultrastructural change induced by an acute bout of plyometric exercise to determine which type of muscle fibers is predominantly damaged. Design: Descriptive laboratory study. Setting: Research laboratory. Patients or Other Participants: Eight healthy, untrained individuals (age = 22 ± 1 years, height = 179.2 ± 6.4 cm, weight = 78.9 ± 5.9 kg). Intervention(s): Partici…