Search results for "Isolation"

Beetwen Berlin and Moscow: Polish Western Thought in the Twenty-Year Interwar Period

2020

The article was submitted on 27.02.2020. This article is not primarily focused on presenting arguments and views held by Polish political groups with reference to the territorial shape of the Polish state after the First World War. Instead, its aim is to draw attention to actions taken by these groups towards the defence of Polish western lands. One of the key problems of Poland’s foreign policy after 1918 was the question of relations with its neighbours, chiefly Germany and Russia (and the Soviet Union). For many years, the most serious problem faced by post-Versailles Europe was that of the Germans striving to revise the legal order, to break their political isolation, and return to the …

Peroxynitrite generated from constitutive nitric oxide synthase mediates the early biochemical injury in short-term cultured hepatocytes

2000

AbstractEarly loss of P450 in rat hepatocyte cultures appears directly related to nitric oxide (NO) overproduction. This study provides experimental evidence for the induction – shortly after isolation through the classical procedure – of strong oxidative stress that involves both oxygen-derived and NO-derived species. NO formation at this stage is due to the early activation of liver constitutive NO synthase (cNOS). Immunodetection of nitrated proteins provides direct evidence of endogenous peroxynitrite (PN) formation upon hepatocyte isolation. On the basis of the combined use of dihydrorhodamine 123 and NOS inhibitors, the analysis of the amount, time course and nature of the species inv…

Geographical separation and physiology drive differentiation of microbial communities of two discrete populations of the bat Leptonycteris yerbabuenae

2020

In this paper, we explore how two discrete and geographically separated populations of the lesser long‐nosed bat (Leptonycteris yerbabuenae)—one in central and the other in the Pacific region of Mexico—differ in their fecal microbiota composition. Considering the microbiota–host as a unity, in which extrinsic (as food availability and geography) or intrinsic factors (as physiology) play an important role in the microbiota composition, we would expect differentiation in the microbiota of two geographically separated populations. The Amplicon Sequences Variants (ASVs) of the V4 region of the 16s rRNA gene from 68 individuals were analyzed using alpha and beta diversity metrics. We obtained a …

Impact of biocide treatments on the bacterial communities of the Lascaux Cave.

2009

The Lascaux Cave contains a remarkable set of paintings from the Upper Palaeolithic. Shortly after discovery in 1940, the cave was modified for public viewing and, in 2001, was invaded by a Fusarium solani species complex. Benzalkonium chloride was used from 2001 to 2004 to eliminate the fungal outbreak. In this study, we carried out a sampling in most of the cave halls and galleries. Sequence analysis and isolation methods detected that the most abundant genera of bacteria were Ralstonia and Pseudomonas. We suggest that, as a result of years of benzalkonium chloride treatments, the indigenous microbial community has been replaced by microbial populations selected by biocide application.

Monitoring complex bacterial communities using culture-independent molecular techniques: application to soil environment

2000

Over the last decade, important advances in molecular biology led to the development of culture-independent approaches to describing bacterial communities. These new strategies, based on the analysis of DNA directly extracted from environmental samples, circumvent the steps of isolation and culturing of bacteria, which are known for their selectivity leading to a non-representative view of the extent of bacterial diversity. This review provides an overview of the potentials and limitations of some molecular approaches currently used in microbial ecology. Examples of applications to the study of indigenous soil microbial community illustrate the feasibility and the power of such approaches.

High maternal species density mediates unidirectional heterospecific matings inCalopteryxdamselflies

2012

Hybridization is a well-known phenomenon, but there are still relatively few studies addressing the question of reproductive isolation between related sympatric animal species with largely overlapping ranges. Population density, relative abundance, and operational sex ratio (OSR) are among the factors known to have an influence on the frequency of heterospecific matings in sympatric populations. Here we had two aims. First, we used microsatellite markers and field observations to study the frequency of hybrids, and backcrosses, and the rate of heterospecific matings between two sympatric damselfly species Calopteryx splendens (Harris, 1780) and Calopteryx virgo (Linne, 1758). Second, we inv…

Isolation, structure elucidation, and biological evaluation of the unusual heterodimer chrysoxanthone from the ascomycete IBWF11-95A

2009

Chrysoxanthone, an unusual heterodimer of blennolide A and 2-hydroxychrysophanol linked through a diaryl ether bridge, was isolated from mycelia of the ascomycete IBWF11-95A grown in submerged culture. Its structure was elucidated by two-dimensional NMR spectroscopy. The metabolite shows antibacterial activity against different species with MIC values between 2.5 and 20 μg/mL while also inhibiting the growth of several fungi.

Isolation and Functional Analysis of Organelles

2006

Fractal Dimension Logarithmic Differences Method for Low Voltage Series Arc Fault Detection

2021

Series arc faults introduce singularities in the current signal and changes over time. Fractal dimension can be used to characterize the dynamic behaviour of the current signal by providing a degree of signal chaos. This measure of irregularity exhibits changes in signal behaviour that can suitably be used as a basis for series arc fault detection. In this paper, an efficient low voltage series arc fault detection method based on the logarithmic differences of the estimate of the fractal dimension of the current signal using the multiresolution length-based method is presented. The discrete wavelet transform and the hard thresholding denoising with the universal threshold are also used. Exp…

Sample Preparation to Determine Pharmaceutical and Personal Care Products in an All-Water Matrix: Solid Phase Extraction

2020

© 2020 by the authors. Pharmaceuticals and personal care products (PPCPs) are abundantly used by people, and some of them are excreted unaltered or as metabolites through urine, with the sewage being the most important source to their release to the environment. These compounds are in almost all types of water (wastewater, surface water, groundwater, etc.) at concentrations ranging from ng/L to µg/L. The isolation and concentration of the PPCPs from water achieves the appropriate sensitivity. This step is mostly based on solid-phase extraction (SPE) but also includes other approaches (dispersive liquid-liquid microextraction (DLLME), buckypaper, SPE using multicartridges, etc.). In this rev…