Search results for "K-12"
showing 10 items of 10 documents
LrhA as a new transcriptional key regulator of flagella, motility and chemotaxis genes in Escherichia coli
2002
The function of the LysR-type regulator LrhA of Escherichia coli was defined by comparing whole-genome mRNA profiles from wild-type E. coli and an isogenic lrhA mutant on a DNA microarray. In the lrhA mutant, a large number (48) of genes involved in flagellation, motility and chemotaxis showed relative mRNA abundances increased by factors between 3 and 80. When a representative set of five flagellar, motility and chemotaxis genes was tested in lacZ reporter gene fusions, similar factors for derepression were found in the lrhA mutant. In gel retardation experiments, the LrhA protein bound specifically to flhD and lrhA promoter DNA (apparent K-D approximate to 20 nM), whereas the promoters of…
Structure-based analyses of Salmonella RcsB variants unravel new features of the Rcs regulon
2021
18 páginas, 7 figuras, 2 tablas
Salmonella bongori Provides Insights into the Evolution of the Salmonellae
2011
The genus Salmonella contains two species, S. bongori and S. enterica. Compared to the well-studied S. enterica there is a marked lack of information regarding the genetic makeup and diversity of S. bongori. S. bongori has been found predominantly associated with cold-blooded animals, but it can infect humans. To define the phylogeny of this species, and compare it to S. enterica, we have sequenced 28 isolates representing most of the known diversity of S. bongori. This cross-species analysis allowed us to confidently differentiate ancestral functions from those acquired following speciation, which include both metabolic and virulence-associated capacities. We show that, although S. bongori…
Comparing technology acceptance of K‐12 teachers with and without prior experience of learning management systems : A Covid‐19 pandemic study
2021
Covid-19 pandemic has caused a massive transformation in K-12 settings towards online education. It is important to explore the factors that facilitate online teaching technology adoption of teachers during the pandemic. The aim of this study was to compare Learning Management System (LMS) acceptance of Finnish K-12 teachers who have been using a specific LMS as part of their regular teaching before the Covid-19 pandemic (experienced group) and teachers who started using it for emergency remote teaching during the pandemic (inexperienced group). Based on the Unified Theory of Acceptance and Use of Technology framework, a self-report questionnaire was administered to 196 teachers (nexperienc…
Digital competence across boundaries - beyond a common Nordic model of the digitalisation of K-12 schools?
2021
This paper explores policy related to digital competence and the digitalisation of Nordic K-12 schools. Anchored in some key transnational policies on digital competence, it describes some current Nordic movements in the national policies of Denmark, Finland, Norway and Sweden. The concept of boundary objects is used as an analytical lens, for understanding digital competence as a plastic and temporal concept that can be used to discuss the multi-dimensional translation of this concept in these Nordic countries. The paper ends with a discussion of the potential to view digital competence as a unifying boundary object that, with its plasticity, temporality and n-dimensionality, can show sign…
Algorithms and Logic as Programming Primers
2019
To adapt all-immersive digitalization, the Finnish National Curriculum 2014 (FNC-2014) ‘digi-jumps’ by integrating programming into elementary education. However, applying the change to mathematics teachers’ everyday praxis is hindered by a too high-level specification. To elaborate FNC-2014 into more concrete learning targets, we review the computer science syllabi of countries that are well ahead, as well as the education recommendations set by computer science organizations, such as ACM and IEEE. The whole mathematics syllabus should be critically viewed in the light of these recommendations and feedback collected from software professionals and educators. The feedback reveals an imbalan…
On the impact of computer science outreach events on K-12 students
2016
Many countries have begun to adopt computer science (CS) and computational thinking (CT) into national curricula of compulsory education and upper secondary education. It is argued that learning rigorous CS concepts not only secures a workforce for the future’s digital industries but also benefits all students by improving their problem-solving and logical reasoning skills. However, the popularity of CS as a university major declined in the beginning of the 21st century, resulting in the development of a range of student outreach activities to engage young students in the study of computing. This thesis originated from this need to attract and retain students in the CS field. The focus of thi…
Data from: Fight evolution with evolution: plasmid-dependent phages with a wide host range prevent the spread of antibiotic resistances
2013
The emergence of pathogenic bacteria resistant to multiple antibiotics is a serious worldwide public health concern. Whenever antibiotics are applied, the genes encoding for antibiotic resistance are selected for within bacterial populations. This has led to the prevalence of conjugative plasmids that carry resistance genes and can transfer themselves between diverse bacterial groups. In this study, we investigated whether it is feasible to attempt to prevent the spread of antibiotic resistances with a lytic bacteriophage, which can replicate in a wide range of gram-negative bacteria harboring conjugative drug-resistance conferring plasmids. The counter-selection against the plasmid was sho…
The genome sequence of Blochmannia floridanus: Comparative analysis of reduced genomes
2003
Bacterial symbioses are widespread among insects, probably being one of the key factors of their evolutionary success. We present the complete genome sequence of Blochmannia floridanus , the primary endosymbiont of carpenter ants. Although these ants feed on a complex diet, this symbiosis very likely has a nutritional basis: Blochmannia is able to supply nitrogen and sulfur compounds to the host while it takes advantage of the host metabolic machinery. Remarkably, these bacteria lack all known genes involved in replication initiation ( dna A, pri A, and rec A). The phylogenetic analysis of a set of conserved protein-coding genes shows that Bl. floridanus is phylogenetically related to Buch…
Elementary Math to Close the Digital Skills Gap
2018
All-encompassing digitalization and the digital skills gap pressure the current school system to change. Accordingly, to ’digi-jump’, the Finnish National Curriculum 2014 (FNC-2014) adds programming to K-12 math. However, we claim that the anticipated addition remains too vague and subtle. Instead, we should take into account education recommendations set by computer science organizations, such as ACM, and define clear learning targets for programming. Correspondingly, the whole math syllabus should be critically viewed in the light of these changes and the feedback collected from SW professionals and educators. These findings reveal an imbalance between supply and demand, i.e., what is ove…