Search results for "KNO"

Using Local Ecological Knowledge of Fishers to Reconstruct Abundance Trends of Elasmobranch Populations in the Strait of Sicily

2020

Fishers “local ecological knowledge” (LEK) can be used to reconstruct long-term trends of species that are at very low biomass due to overfishing. In this study, we used historical memories of Sicilian fishers to understand their perception of change in abundance of cartilaginous fish in the Strait of Sicily over the last decades. We conducted interviews with 27 retired fishers from Mazara del Vallo harbor (SW Sicily) working in demersal fisheries, using a pre-defined questionnaire with a series of open and fixed questions related to the abundance of sharks and rays. The questionnaire included specific questions about the trends they perceived in catch or by-catch of cartilaginous fish abun…

Interaction Between ABA Signaling and Copper Homeostasis inArabidopsis thaliana

2016

ABA is involved in plant responses to non-optimal environmental conditions, including nutrient availability. Since copper (Cu) is a very important micronutrient, unraveling how ABA affects Cu uptake and distribution is relevant to ensure adequate Cu nutrition in plants subjected to stress conditions. Inversely, knowledge about how the plant nutritional status can interfere with ABA biosynthesis and signaling mechanisms is necessary to optimize stress tolerance in horticultural crops. Here the reciprocal influence between ABA and Cu content was addressed by using knockout mutants and overexpressing transgenic plants of high affinity plasma membrane Cu transporters (pmCOPT) with altered Cu up…

Transcriptomic responses to biotic stresses in Malus x domestica: a meta-analysis study.

2017

AbstractRNA-Seq analysis is a strong tool to gain insight into the molecular responses to biotic stresses in plants. The objective of this work is to identify specific and common molecular responses between different transcriptomic data related to fungi, virus and bacteria attacks in Malus x domestica. We analyzed seven transcriptomic datasets in Malus x domestica divided in responses to fungal pathogens, virus (Apple Stem Grooving Virus) and bacteria (Erwinia amylovora). Data were dissected using an integrated approach of pathway- and gene- set enrichment analysis, Mapman visualization tool, gene ontology analysis and inferred protein-protein interaction network. Our meta-analysis revealed…

Benefits of immune protection versus immunopathology costs: a synthesis from cytokine KO models.

2017

5 pages; International audience; The inflammatory response can produce damage to host tissues and in several infectious diseases the most severe symptoms are due to immunopathology rather than a direct effect of pathogen multiplication. One hypothesis for the persistence of inflammatory damage posits that the benefits of protection towards infection outweigh the costs. We used data on knocked-out (KO) cytokine models [and the corresponding wild-type (WT) controls] to test this hypothesis. We computed differences in pathogen load and host survival between WT and KO and divided them by the WT values. Using this ratio provides an internal control for variation in pathogen species, host strain,…

Flashing light emitting diodes (LEDs) induce proteins, polyunsaturated fatty acids and pigments in three microalgae

2020

As the periodic emission of light pulses by light emitting diodes (LEDs) is known to stimulate growth or induce high value biocompounds in microalgae, this flashing light regime was tested on growth and biochemical composition of the microalgae Nannochloropsis gaditana, Koliella antarctica and Tetraselmis chui. At low flashing light frequencies (e.g., 5 and 50 Hz, Duty cycle = 0.05), a strain-dependent growth inhibition and an accumulation of protein, polyunsaturated fatty acids, chlorophyll or carotenoids (lutein, β-carotene, violaxanthin and neoxanthin) was observed. In addition, a 4-day application of low-frequency flashing light to concentrated cultures increased productivities of eicos…

2019

RNA interference (RNAi) is a powerful tool for studying functions of candidate genes in both model and nonmodel organisms and a promising technique for therapeutic applications. Successful application of this technique relies on the accuracy and reliability of methods used to quantify gene knockdown. With the limitation in the availability of antibodies for detecting proteins, quantitative PCR (qPCR) remains the preferred method for quantifying target gene knockdown after dsRNA treatment. We evaluated how qPCR primer binding site and target gene expression levels affect quantification of intact mRNA transcripts following dsRNA-mediated RNAi. The use of primer pairs targeting the mRNA sequen…

Co-Designing Social Simulation Models For Policy Advise: Lessons Learned From the INFSO-SKIN Study

2019

This paper describes a participatory approach to co-designing social simulation models with policymakers using a case study of modeling European Commission policy. Managing the collaboration of a wide range of individuals or organizations is challenging but increasingly important as policy making becomes more complex. A framework for a co-design process based on a participatory approach is proposed. The framework suggests that the collaborative design should go through the following phases: Identifying user questions, data provision, model discussion for validation, visualization of results and discussing scope and limitations with stakeholders. Key findings are that the co-design process r…

Acceptance and knowledge of evolutionary theory among third-year university students in Spain

2020

The theory of evolution is one of the greatest scientific achievements in the intellectual history of humankind, yet it is still contentious within certain social groups. Despite being as robust and evidence-based as any other notable scientific theory, some people show a strong reluctance to accept it. In this study, we used the Measure of Acceptance of the Theory of Evolution (MATE) and Knowledge of Evolution Exam (KEE) questionnaires with university students from four academic degree programs (Chemistry, English, History, and Biology) of ten universities from Spain to measure, respectively, acceptance and knowledge of evolutionary theory among third-year undergraduate students (nMATE = 9…

Interactions between Climate Change and Infrastructure Projects in Changing Water Resources: An Ethnobiological Perspective from the Daasanach, Kenya

2021

The fast and widespread environmental changes that have intensified in the last decades are bringing disproportionate impacts to Indigenous Peoples and Local Communities. Changes that affect water resources are particularly relevant for subsistence-based peoples, many of whom already suffer from constraints regarding reliable access to safe water. Particularly in areas where water is scarce, climate change is expected to amplify existing stresses in water availability, which are also exacerbated by multiple socioeconomic drivers. In this paper, we look into the local perceptions of environmental change expressed by the Daasanach people of northern Kenya, where the impacts of climate change …

Coping with Difficult Weather and Snow Conditions : Reindeer herders’ views on climate change impacts and coping strategies

2016

Winter is a critical season for reindeer herding, with the amount and quality of snow being among the most important factors determining the condition of reindeer and the annual success of the livelihood. Our first aim was to model the future (2035–2064) snow conditions in northern Finland, especially the quantities related to ground ice and/or ice layers within the snow pack, exceptionally deep snow and late snow melt. Secondly, we studied the strategies by which herders cope with the impacts of difficult weather and snow conditions on herding by interviewing 21 herders. SNOWPACK simulations indicate that snow cover formation will be delayed by an average of 19 days and snow will melt 16 d…