Search results for "Karyotype"

Spermatocyte chromosome analysis of the slugLehmannia melitensis(Lessona and Pollonera, 1891) (Mollusca, Pulmonata) using conventional, NOR- and C-ba…

1993

SUMMARYDiploid number 2n = 40 and haploid number n = 20 for the slug Lehmannia melitensis have been determined. These chromosome values being considerably different from those reported for slugs belonging to other limacid genera support the notion that the genus Lehmannia is particular within the family Limacidae. Nucleolus organizer regions (NORs) and C-banding pattern of L. melitensis are described.

Flow Cytometry and Karyotype Analysis ofD. melanogasterEye Disc Cells

2008

The developing Drosophila eye-antennal disc is a particularly suited system for the genetic and cellular studies of complex biological processes. Methods to analyze Drosophila eye discs by flow cytometry are mainly based on the dissociation of tissues with trypsin. Dissociation operated by trypsin is very effective, though it causes a lot of stress to live cells often compromising the use of treated cells for further analyses. Here, we report a method to produce dissociated eye-disc cells that retain cell-membrane markers and that can be used for flow cytometry and cytological analysis of mitotic chromosomes. The method described is a great complementing tool for the cellular characterizati…

Cytogenetic characterization ofBrachidontes pharaonis(Fisher P., 1870): Karyotype, banding and fluorescentin situhybridization (fish) (Mollusca: Biva…

2000

Abstract The mussel Brachidontes pharaonis (Fisher P., 1870) (Bivalvia: Mytilidae) has a diploid chromosomal set of 28 made up of 14 pairs of which eight are mono-armed (ST) and six bi-armed (M+SM). Fourteen bivalents occur in spermatocytes both at pachytene and metaphase-I. The use of combined silver and CMA3 staining reveals that nucleolus organizer regions (NORs) are located terminally on the long arm of a small subtelocentric chromosome pair (pair 14) and are compartmentalized in GC base pairs. A Paracentrotus lividus (Echinodermata) 4.3 kilobase (kb) rDNA probe (prR14) consisting of sequences from the 3′ end of 18S rDNA to the 3′ end of 26S rDNA was used to map the rDNA loci of B. phar…

Microduplications At 22q11.21 are Associated with Classic Bladder Exstrophy

2010

Purpose Classic exstrophy of the bladder (CBE) is part of the exstrophy-epispadias complex (EEC), a spectrum of urogenital anomalies in which part or all of the distal urinary tract fails to close. Familial occurrence has been observed, and previous studies have suggested an underlying multifactorial mode of inheritance. To date, no causative genetic or non-genetic factor has been unequivocally identified in humans. The present study aimed to identify microaberrations characterized by loss or gain of genomic material that contribute to the EEC at a genome-wide level. Material and Methods Molecular karyotyping, utilizing 549,839 single nucleotide polymorphisms (SNPs) with an average spacing …

Healthy 12-year-old boy with mosaic inv dup(15)(q13)

2006

Mapping CAP-A satellite DNAs by FISH in Sapajus cay paraguay and S. macrocephalus (Platyrrhini, Primates)

2023

Satellite DNAs such as Cap-A sequences are potentially informative taxonomic and phylogenetic markers useful for characterizing primate genomes. They have also been used as cytogenetic markers facilitating species identification in many taxa. The aim of this work is to map Cap-A sequences by FISH (fluorescent in situ hybridization) on two Platyrrhini (Primates) species genomes, Sapajus cay paraguay and S. macrocephalus, in order to study their distribution pattern on chromosomes. The Cap-A probes showed bright signals with almost the same interstitial pattern of distribution in correspondence with C and CMA3 rich regions on six pairs of chromosomes in both Sapajus species. An additional pai…

Exploring Evolution in Ceboidea (Platyrrhini, Primates) by Williams-Beuren Probe (HSA 7q11.23) Chromosome Mapping

2007

The ancestral platyrrhine karyotype was characterised by a syntenic association of human 5 and a small segment of human 7 orthologues. This large syntenic association has undergone numerous rearrangements in various phylogenetic lines. We used a locus-specific molecular cytogenetic approach to study the chromosomal evolution of the human 7q11.23 orthologous sequences (William-Beuren syndrome, WS) in various Ceboidea (Platyrrhini) species. The fluorescent in situ hybridisation of the WS probe revealed a two-way pattern of chromosomal organisation that suggests various evolutionary scenarios. The first pattern (seen in Callimico and Saimiri ) includes a fairly simple disruption of the 7/5 syn…

.Chromosome sex determination and Y-autosome fusion in Blennius tentacularis Brunnich, 1765 (Pisces, Blennidae)

1987

Diploid modal numbers of 2n=48 for females, and 2n=48 and 2n=47 for males of Blennius tentacularis caught in the Gulf of Palermo (Sicily) are established. Chromosome sex-determination is proposed for this species in which a Y-autosome fusion has been found.

Karyotype analysis of the sea urchinParacentrotus lividus (Echinodermata): evidence for a heteromorphic chromosome sex mechanism

1996

A consistent diploid number of 2n = 36 was determined for the sea urchinParacentrotus lividus from the Gulf of Palermo by analysis of mitotic chromosomes of both early developing embryos and male gonads. The haploid numbern = 18 was determined by counts of spermatocyte bivalents at diakinesis. A heteromorphic chromosome sex mechanism of the XY type is likely present in this species. This is indicated by the occurrence of a chromosomal pair, pair No. 2, which is heteromorphic in both morphology and size in about 50% of the mitotic figures (metaphases and anaphases) of einbryos. In addition, heteromorphism of the same pair of chromosomes occurred during spermatogonial metaphases in the five m…

Chromosomal nucleolar organizer region (NOR) phenotypes in nine species of the genus Ophryotrocha (Polychaeta: Dorvilleidae)

1995

Chromosomal nucleolar organizer region (NOR) phenotypes have been characterized in nine species of the genus Ophryotrocha (Polychaeta: Dorvilleidae), namely O. notoglandulata, O. sp. macrovifera, O. sp. labronica pacifica, O. labronica labronica, O. puerilis puerilis, O. diadema, O. sp. robusta, O. gracills and O. hartmanni. Irrespective of chromosome number and morphology, Ag positive regions were terminally located in all but one species, O. diadema, where the NORs were pericentromerical in a metacentric pair. The presence of a single chromosome pair bearing NOR in invertebrates is considered an ancestral trait. According to this assumption, O. sp. robusta, O. dialema, and perhaps O. p. p…