Search results for "Kindling"
showing 10 items of 10 documents
Regulation of vascular function and inflammation via cross talk of reactive oxygen and nitrogen species from mitochondria or nadph oxidase—implicatio…
2020
Oxidative stress plays a key role for the development of cardiovascular, metabolic, and neurodegenerative disease. This concept has been proven by using the approach of genetic deletion of reactive oxygen and nitrogen species (RONS) producing, pro-oxidant enzymes as well as by the overexpression of RONS detoxifying, antioxidant enzymes leading to an amelioration of the severity of diseases. Vice versa, the development and progression of cardiovascular diseases is aggravated by overexpression of RONS producing enzymes as well as deletion of RONS detoxifying enzymes. We have previously identified cross talk mechanisms between different sources of RONS, which can amplify the oxidative stress-m…
Toward evidence-based severity assessment in rat models with repeated seizures: I. Electrical kindling
2018
Objective: Rodent epilepsy models can significantly contribute to our understanding of pathophysiological mechanisms and to validation of biomarker and target candidates. Evidence-based severity assessment is a presupposition for the ethical evaluation of animal experimentation allowances as well as for the development of efficacious refinement concepts. Methods: Aiming to improve our understanding of the impact of experimental procedures and repeated seizures, we have completed a comprehensive behavioral and biochemical analysis assessing various parameters that can inform about the influence of an electrical kindling paradigm on well-being in rats. Thereby, we have focused on the immediat…
Design of composite measure schemes for comparative severity assessment in animal-based neuroscience research: A case study focussed on rat epilepsy …
2020
PLOS ONE 15(5), e0230141 (2020). doi:10.1371/journal.pone.0230141
NMDA-GABA interactions in an animal model of behaviour: a gating mechanism from motivation toward psychotic-like symptoms
1994
We studied the effects of desipramine, alprazolam, muscimol and dizocilpine (MK-801) (alone or associated with desipramine) in the forced swimming test in rats after long-lasting termination of chronic exposure to vehicle and pentylenetetrazol. Sensitisation with pentylenetetrazol was ineffective in changing immobility time in the forced swimming test compared to vehicle treatment; pentylenetetrazol enhanced the anti-immobility effect of desipramine, abolished the anti-immobility effect of alprazolam and did not affect the anti-immobility effect of muscimol. MK-801 at the dose that did not modify immobility time in vehicle-treated rats and in pentylenetetrazol-treated animals strongly poten…
Memantine presents different effects from MK-801 in motivational and physical signs of morphine withdrawal
2003
Adaptive changes in neural systems due to chronic opiate exposure are related to the neural plasticity phenomenon, NMDA receptors being implicated in these processes, e.g. tolerance, dependence or withdrawal. In this work, we investigated the effect of two non-competitive NMDA antagonists, memantine and MK-801, in motivational (Conditioned Place Aversion paradigm, CPA) and physical aspects of morphine withdrawal. After the induction of morphine dependence, animals in which the CPA was studied, received memantine (5 and 10 mg/kg) or MK-801 (0.3-0.006 mg/kg) either during the acquisition (conditioning) or expression (test) phase of this procedure. Both drugs were capable of inhibiting conditi…
Effects of desipramine and alprazolam in the forced swim test in rats after long-lasting termination of chronic exposure to picrotoxin and pentylenet…
1993
Abstract Rats were treated for 5 weeks with three subconvulsant doses of picrotoxin (PTX) and pentylenetetrazol (PTZ) per week to induce a persistent reduction of the GABA A receptor function which results in chemical kindling. Fifteen days after termination of this treatment schedule, the effect of desipramine (DMI) and alpraxolam (ALP) on immobility time in the forced swim test (FST) was evaluated. Chronic PTX and PTZ did not alter the immobility time. Acute PTX and PTZ reduced the immobility of rats chronically treated with vehicle but not of those exposed chronically to PTX and PTZ. Chronic PTX did not influence the anti-immobility effect of DMI, but blocked that of ALP. Chronic PTZ mar…
Continuous intra-amygdalar infusion of GABA in the amygdala kindling model of epilepsy in rat.
2003
Objective: To explore the effect of continuous intra-amygdalar infusion of GABA in the amygdala kindling model of epilepsy in rat. Methods: An electrode and cannula complex was implanted in adult male Wistar rats, the electrode being targeted to the left basolateral amygdala. The animals were subjected to a standard kindling procedure. Osmotic minipumps filled with either GABA or mannitol were connected to cannulas and allowed to infuse during 7 days. Kindling experiments measuring after-discharge and seizure thresholds, seizure severity and duration, and behavioral toxicity were performed before, during and after the drug infusion period. Results: Both after-discharge and seizure threshold…
Epoch Parameterization by Gabor Atom Density in Experimental Epilepsy
2007
An Electrocorticogram (ECoG), during an epilepsy episode can change dramatically from the normal state into a high amplitude low frequency signal and suddenly return to the normal sate. It is possible to identify some stages in the epilepsy seizure, the most representative of them: basal, preictal, ictal and posictal. ECoG are highly non periodical signals, so they are analyze with T-F algorithms, in order to follow up its frequency evolution through the seizure stages. Each seizure stage has different frequency components and they show up at different time. Experimental epilepsy produce by kindling model in rats is used; signals are recorded at cortex level. The ECoG is decompose by means …
Epilepsy stages diagnosis by Gabor atom density according to their aspect ratio
2008
During epilepsy seizure Electrocorticogram (ECoG) may change dramatically from a nearly chaotic signal (basal state) into a highly synchronized signal during a seizure, characterized by high amplitude and low frequency, and suddenly go back to the basal sate, making hard to identify them from time series. The epileptic seizure shows some stages as it is evolving, the here studied are: basal, preictal, ictal and posictal. As most of the bioelectrical signal, ECoG is a highly non periodical signal, so the most suitable techniques to analyze them are the Time-Frequency algorithms (T-F), allowing to follow up its frequency evolution through the seizure. Each seizure stage has a set of frequency…
Effect of Acetaldehyde Intoxication and Withdrawal on NPY Expression: Focus on Endocannabinoidergic System Involvement
2014
Acetaldehyde (ACD), the first alcohol metabolite, plays a pivotal role in the rewarding, motivational and addictive properties of the parental compound. Many studies have investigated the role of ACD in mediating neurochemical and behavioral effects induced by alcohol administration, but very little is known about the modulation of neuropeptide systems following ACD intoxication and withdrawal. Indeed the neuropeptide Y (NPY) system is altered during alcohol withdrawal in key regions for cerebrocortical excitability and neuroplasticity. The primary goal of this research was to investigate the effects of ACD intoxication and withdrawal by recording rat behavior and by measuring neuropeptide …