Search results for "LSM"
showing 10 items of 57 documents
Prefoldins contribute to maintaining the levels of the spliceosome LSM2–8 complex through Hsp90 in Arabidopsis
2020
14 p.-7 fig.-2 tab.
The endophytic microbiota of Citrus limon is transmitted from seed to shoot highlighting differences of bacterial and fungal community structures
2021
AbstractCitrus limon (L.) Burm. F. is an important evergreen fruit crop whose rhizosphere and phyllosphere microbiota have been characterized, while seed microbiota is still unknown. Bacterial and fungal endophytes were isolated from C. limon surface-sterilized seeds. The isolated fungi—belonging to Aspergillus, Quambalaria and Bjerkandera genera—and bacteria—belonging to Staphylococcus genus—were characterized for indoleacetic acid production and phosphate solubilization. Next Generation Sequencing based approaches were then used to characterize the endophytic bacterial and fungal microbiota structures of surface-sterilized C. limon seeds and of shoots obtained under aseptic conditions fr…
The seed endosphere of Anadenanthera colubrina is inhabited by a complex microbiota, including Methylobacterium spp. and Staphylococcus spp. with pot…
2018
Background and aims: Plant seeds are emerging micro–habitats, whose importance as reservoir and vector of beneficial microbes just begins to be recognized. Here we aimed to characterize the bacterial microbiota of the Anadenanthera colubrina seed endosphere, with special focus to beneficial traits and to the colonization pattern. Methods: Cultivation–dependent (isolation from surface–sterilized seeds) and cultivation–independent (pyrosequencing of 16S rRNA gene from metagenomic seed DNA) analyses, functional tests and microscopical investigations (fluorescence in situ hybridization coupled with confocal laser scanning microscopy (FISH-CLSM) were performed. Results: We isolated several Methy…
Manual de la literatura hispanoamericana
2019
The combination of liver stiffness measurement and NAFLD fibrosis score improves the noninvasive diagnostic accuracy for severe liver fibrosis in pat…
2014
Background & Aims The accuracy of noninvasive tools for the diagnosis of severe fibrosis in patients with nonalcoholic fatty liver disease(NAFLD) in clinical practice is still limited. We aimed at assessing the diagnostic performance of combined noninvasive tools in two independent cohorts of Italian NAFLD patients. Methods We analysed data from 321 Italian patients(179 Sicilian-training cohort, and 142 northern Italy-validation cohort) with an histological diagnosis of NAFLD. Severe fibrosis was defined as fibrosis ≥ F3 according to Kleiner classification. The APRI, AST/ALT, BARD, FIB-4, and NFS scores were calculated according to published algorithms. Liver stiffness measurement(LSM) was …
Ārvalstu tiešās investīcijas Latvijā un algu nevienlīdzība starp pašmāju un ārvalstu uzņēmumiem
2022
Līdz ar nepārtrauktu kapitāla aprites pieaugumu pēdējo gadu laikā ir ievērojami palielinājusies nodarbinātība ārvalstu īpašumā esošajos uzņēmumos. Pieejamā literatūra par algu līmeņu atšķirībām starp pašmāju un ārvalstu uzņēmumiem atklāj pretējus rezultātus. Darba mērķis ir noskaidrot, kā ārvalstu kapitāla klātbūtne uzņēmumos ietekmē darba algu atšķirības starp vietējiem un ārvalstu uzņēmumiem Latvijā. Pētījuma rezultāti atklāj, ka nepastāv cieša sakarība starp augstāku darba samaksu Latvijā reģistrētos uzņēmumos, kuros ir ārvalstu tiešās investīcijas. Rezultāti liecina arī, ka 54% no profesijām augstākas algas ir pašmāju īpašumā esošajos uzņēmumos, un vienas profesijas darba samaksa vidēji…
Endophytes from medicinal plants’ seeds: exploring new reservoirs of bioactive molecules
Plant-associated microorganisms have recently gained more attention for their influence on plant health and biotechnological relevance. Bioactive molecules have been already isolated from plant-associated bacteria. Seed-borne bacterial endophytes have not been much explored though. Such endophytes are particularly important, since they can influence germination and be transmitted from generation to generation. Interestingly, seed endophytes from medicinal plants could influence the production of molecules with therapeutic properties. Bacterial endophytic strains were extracted from surface-sterilized seeds of the medicinal plant Echinacea purpurea and their 16S rRNA genes were sequenced. Th…
Dažādas izcelsmes medus autentiskuma novērtējums
2018
Darbā ir apkopota informācija par medu, tā viltošanu un viltojumu noteikšanas metodēm. Pētītas publikācijas par proteīnu izdalīšanu, vieglo stabilo izotopu masspektrometriju un aplūkotas hromatogrāfijas metodes cukuru noteikšanai. Tika veikta proteīnu izdalīšana no dažādas izcelsmes un viltotu medus paraugiem. Tika veikta vieglo stabilo izotopu attiecību analīze medus paraugos un viltošanai izmantotajām piedevām. Atsevišķi tika pētīti ar hromatogrāfijas metodi viltoti medus paraugi. Iegūtie rezultāti tika izmantoti, lai pētītu atšķirības starp dažādu reģionu izcelsmes medus paraugiem, kā arī, lai atrastu liecības par apzināti viltotiem medus paraugiem un pārliecinātos par to autentiskumu.
A method for measuring mitochondrial mass and activity
2007
Mitochondria, responsible for the energy-generating process essential for the cell metabolism, differ for the number, localization and activity in animal cells and tissues in relation to the energetic needs. Using fluorescent probes specific for mitochondria, Mitotracker Green (MTG) and Orange (MTO), and Confocal Laser-Scanning Microscope (CLSM), we elaborated a method to measure in vivo the mitochondrial mass and activity, in sea urchin Paracentrotus lividus eggs and embryos. The analysis of captured images, revealed a variation of mitochondrial distribution and an increase of activity after fertilization.