Search results for "Labeling"

Sentence Induced Transformations in Conceptual Spaces

2008

The proposed work illustrates how "primitive concepts" can be automatically induced from a text corpus. The primitive concepts are identified by the orthonormal axis of a "conceptual" space induced by a methodology inspired to the latent semantic analysis approach. The methodology represents a natural language sentence by means of a set of rotations of an orthonormal basis in the "conceptual"space. The rotations, triggered by the sequence of words composing the sentence and realized by means of geometric algebra rotors, allow to highlight "conceptual" relations that can arise among the primitive concepts.

Diventare normali. Teorie, analisi e applicazioni interazioniste della devianza e del crimine

2016

La devianza e il crimine sono condizioni «oggettive»? Perché si manifestano in maniera diversa nel tempo e nei vari contesti socio-culturali? Si tratta di meri «fatti» registrati al loro occorrere nelle varie fonti statistiche? Perché mai a ogni denuncia non segue necessariamente un arresto? Perché consideriamo minaccioso il «burquini» e non ci preoccupiamo più delle discariche abusive di rifiuti tossici? Perché si stigmatizza lo stupratore e, in taluni casi, si arriva ad ammirare il manager d’azienda corrotto? Devianti e «normali» sono così diversi tra loro? Devianti e criminali si diventa o si nasce? Sicuro che alcuni non riuscirebbero mai a compiere un’azione deviante oppure siamo tutti …

Comparison of the exoproducts of gram-negative bacteria by SDS-page

1985

The protein exoproducts released during exponential growth of Gram-negative bacteria were analysed and compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-Page). The following bacterial strains were tested: Escherichia coli, Klebsiella pneumoniae, Citrobacter freundii, Enterobacter cloacae, Serratia liquefaciens, Serratia rubidaea, Proteus mirabilis, Proteus vulgaris, Salmonella minnesota, Pseudomonas aeruginosa and Pseudomonas fluorescens. It is demonstrated by SDS-Page that members of one species show identical protein pattern, whereas different species show besides comparable protein bands a species characteristic pattern. All members of Enterobacteriaceae were sho…

Infrared Difference Spectroscopy of Proteins: From Bands to Bonds

2020

Infrared difference spectroscopy probes vibrational changes of proteins upon their perturbation. Compared with other spectroscopic methods, it stands out by its sensitivity to the protonation state, H-bonding, and the conformation of different groups in proteins, including the peptide backbone, amino acid side chains, internal water molecules, or cofactors. In particular, the detection of protonation and H-bonding changes in a time-resolved manner, not easily obtained by other techniques, is one of the most successful applications of IR difference spectroscopy. The present review deals with the use of perturbations designed to specifically change the protein between two (or more) functional…

3D Morphology, ultrastructure and development of Ceratomyxa puntazzi stages: first insights into the mechanisms of motility and budding in the Myxozo…

2012

Free, amoeboid movement of organisms within media as well as substrate-dependent cellular crawling processes of cells and organisms require an actin cytoskeleton. This system is also involved in the cytokinetic processes of all eukaryotic cells. Myxozoan parasites are known for the disease they cause in economical important fishes. Usually, their pathology is related to rapid proliferation in the host. However, the sequences of their development are still poorly understood, especially with regard to pre-sporogonic proliferation mechanisms. The present work employs light microscopy (LM), electron microscopy (SEM, TEM) and confocal laser scanning microscopy (CLSM) in combination with specific…

Radio k-Labelings for Cartesian Products of Graphs

2005

International audience; Frequency planning consists in allocating frequencies to the transmitters of a cellular network so as to ensure that no pair of transmitters interfere. We study the problem of reducing interference by modeling this by a radio k-labeling problem on graphs: For a graph G and an integer k ≥ 1, a radio k-labeling of G is an assignment f of non negative integers to the vertices of G such that |f(x)−f(y)| ≥ k+1−dG(x,y), for any two vertices x and y, where dG(x,y) is the distance between x and y in G. The radio k-chromatic number is the minimum of max{f(x)−f(y):x,y ∈ V(G)} over all radio k-labelings f of G. In this paper we present the radio k-labeling for the Cartesian pro…

Staining of immunoblots by immunochromatography.

1994

GFP immunogold staining, from light to electron microscopy, in mammalian cells.

2012

GFP has emerged as an important reporter for monitoring gene expression, protein localization, cell transformation and cell lineage. The development of GFP as a marker in many different biological systems has emphasized the need to image GFP at high resolution. GFP immunogold labeling with colloidal gold particles becomes essential for electron microscopy (EM) ultrastructural detection. Because of the small size, colloidal gold particles require silver enhancement, a procedure to increase the size of the particle as well as gold toning to stabilize the silver layer. GFP preembedding immunogold staining enables high quality cellular-ultrastructural EM analysis mainly for two reasons, on one …

Is immunohistochemistry more sensitive than hematoxylin-eosin staining for identifying perineural or lymphovascular invasion in oral squamous cell ca…

2021

This study aimed to analyze whether immunohistochemistry (IHC) is more sensitive than hematoxylin-eosin (H&E) staining for identifying perineural invasion (PNI) or lymphovascular invasion (LVI) in oral squamous cell carcinoma (OSCC). In this systematic review and meta-analysis (Prospective Register of Systematic Reviews ? CRD 42021256515), data were obtained from six databases (PubMed, Scopus, LILACS, Web of Science, EBSCO, LIVIVO, Embase) and the grey literature. Cross-sectional observational studies of the diagnostic sensitivity of IHC for PNI and LVI were included. Studies were selected in two phases: first collection and reference retrieval. The Quality Assessment of Diagnostic Accuracy…

ANTIMICROBIAL AND ANTISTAPHYLOCOCCAL BIOFILM ACTIVITY FROM THE SEA URCHIN PARACENTROTUS LIVIDUS

2009

Aims: Staphylococcal biofilm-associated infections are resistant to conventional antibiotics. Consequently, new agents are needed to treat them. With this aim, we focused on the effector cells (coelomocytes) of the sea urchin Paracentrotus lividus immune system. Methods and Results: We tested the activity of the 5-kDa peptide fraction of the cytosol from coelomocytes (5-CC) against a group of Gram-positive, Gram-negative bacteria and fungi. We determined minimal inhibitory concentrations (MICs) ranging from 253.7 to 15.8 mg ml(-1). We observed an inhibitory activity and antibiofilm properties of 5-CC against staphylococcal biofilms of reference strains Staphylococcus epidermidis DSM 3269 an…