Search results for "Laboratory"

showing 10 items of 923 documents

The COPD multi-dimensional phenotype: A new classification from the STORICO Italian observational study.

2019

BackgroundThis paper is aimed to (i) develop an innovative classification of COPD, multi-dimensional phenotype, based on a multidimensional assessment; (ii) describe the identified multi-dimensional phenotypes.MethodsAn exploratory factor analysis to identify the main classificatory variables and, then, a cluster analysis based on these variables were run to classify the COPD-diagnosed 514 patients enrolled in the STORICO (trial registration number: NCT03105999) study into multi-dimensional phenotypes.ResultsThe circadian rhythm of symptoms and health-related quality of life, but neither comorbidity nor respiratory function, qualified as primary classificatory variables. Five multidimension…

MalePulmonologyPhysiologyComorbidityAnxietyPathology and Laboratory MedicineCohort StudiesPulmonary Disease Chronic ObstructiveMathematical and Statistical TechniquesQuality of lifeMedicine and Health SciencesCoughingCluster AnalysisRespiratory functionPublic and Occupational HealthAged 80 and overCOPDMultidisciplinaryDepressionApplied MathematicsSimulation and ModelingQStatisticsRMiddle AgedExploratory factor analysisCircadian RhythmBody FluidsCircadian RhythmsPhenotypeItalyPhysical SciencesAnxietyMedicineFemalemedicine.symptomAnatomyFactor AnalysisAlgorithmsCohort studyResearch Articlemedicine.medical_specialtyScienceMemory EpisodicChronic Obstructive Pulmonary DiseaseSettore MED/10 - Malattie Dell'Apparato RespiratorioResearch and Analysis MethodsClustering AlgorithmsSigns and SymptomsDiagnostic MedicineInternal medicinemedicineCOPDHumansStatistical MethodsAgedbusiness.industryBiology and Life SciencesPhysical Activitymedicine.diseaseComorbidityrespiratory tract diseasesMucusDyspneaCoughQuality of LifeObservational studybusinessFactor Analysis StatisticalSleepPhysiological ProcessesChronobiologymultiple phenotypesMathematicsPloS one
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Mechanical strain causes adaptive change in bronchial fibroblasts enhancing profibrotic and inflammatory responses

2016

Asthma is characterized by periodic episodes of bronchoconstriction and reversible airway obstruction; these symptoms are attributable to a number of factors including increased mass and reactivity of bronchial smooth muscle and extracellular matrix (ECM) in asthmatic airways. Literature has suggested changes in cell responses and signaling can be elicited via modulation of mechanical stress acting upon them, potentially affecting the microenvironment of the cell. In this study, we hypothesized that mechanical strain directly affects the (myo)fibroblast phenotype in asthma. Therefore, we characterized responses of bronchial fibroblasts, from 6 normal and 11 asthmatic non-smoking volunteers,…

MalePulmonologyPulmonary FibrosisAdult; Asthma; Biomechanical Phenomena; Bronchi; Case-Control Studies; Female; Fibroblasts; Humans; Male; Pneumonia; Pulmonary Fibrosis; Stress Mechanical; Medicine (all); Biochemistry Genetics and Molecular Biology (all); Agricultural and Biological Sciences (all)Glycobiologylcsh:MedicinePathology and Laboratory MedicineBiochemistryAnimal CellsMedicine and Health Scienceslcsh:ScienceImmune ResponseMusculoskeletal SystemConnective Tissue CellsSmooth MusclesMusclesMedicine (all)Extracellular MatrixBiomechanical PhenomenaConnective TissueFibroblastProteoglycansFemaleCellular TypesAnatomyCellular Structures and OrganellesCase-Control StudieResearch ArticleHumanAdultPulmonary FibrosiImmunologyBronchiSigns and SymptomsExtraction techniquesDiagnostic MedicineHumansInflammationBiochemistry Genetics and Molecular Biology (all)Settore BIO/16 - Anatomia Umanalcsh:RBiology and Life SciencesProteinsCell BiologyPneumoniaFibroblastsRNA extractionAsthmaResearch and analysis methodsBiological TissueAgricultural and Biological Sciences (all)Case-Control Studieslcsh:QStress MechanicalCollagens
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Psychometric Assessment of the Japanese Version of the Zurich Claudication Questionnaire (ZCQ): Reliability and Validity.

2016

Purpose The Zurich Claudication Questionnaire (ZCQ) is a self-administered measure to evaluate symptom severity, physical function, and surgery satisfaction in lumbar spinal stenosis (LSS). The purpose of this study is to assess the psychometric properties of the Japanese ZCQ in LSS patients. Methods LSS patients who are scheduled to undergo surgery were recruited from 12 facilities. Responses to several questionnaires, including the Japanese ZCQ; the visual analogue scale (VAS) to evaluate the degree of pain in the buttocks/legs, numbness in the buttocks/legs, and low back pain; the Oswestry Disability Index (ODI); and the SF-36v2, were collected before surgery and again 3 months after sur…

MaleQuestionnairesResearch ValidityPsychometricsSocial Scienceslcsh:MedicinePathology and Laboratory Medicine0302 clinical medicineSpinal StenosisJapanSurveys and QuestionnairesMedicine and Health SciencesPsychology030212 general & internal medicinelcsh:ScienceMusculoskeletal SystemStenosisMultidisciplinaryLumbar VertebraeMiddle AgedResearch AssessmentLow back painOswestry Disability IndexPatient SatisfactionResearch DesignLegsFemalemedicine.symptomAnatomyResearch Articlemedicine.medical_specialtyPsychometricsVisual analogue scaleConcurrent validityLower Back PainPainSurgical and Invasive Medical ProceduresResearch and Analysis Methods03 medical and health sciencesPatient satisfactionPhysical medicine and rehabilitationSigns and SymptomsCronbach's alphaDiagnostic MedicineMental Health and PsychiatrymedicineHumansAgedSurvey Researchbusiness.industryLimbs (Anatomy)lcsh:RBiology and Life SciencesPhysical therapylcsh:QbusinessClaudication030217 neurology & neurosurgeryPLoS ONE
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The effectiveness of peer-support for people living with HIV: A systematic review and meta-analysis

2021

Background The practice of involving people living with HIV in the development and provision of healthcare has gained increasing traction. Peer-support for people living with HIV is assistance and encouragement by an individual considered equal, in taking an active role in self-management of their chronic health condition. The objective of this systematic review was to assess the effects of peer-support for people living with HIV. Methods We conducted a systematic review in accordance with international guidelines. Following systematic searches of eight databases until May 2020, two reviewers performed independent screening of studies according to preset inclusion criteria. We conducted ri…

MaleRNA virusesEpidemiologyHIV InfectionsPeer supportPathology and Laboratory MedicineMathematical and Statistical TechniquesImmunodeficiency VirusesHealth careMedicine and Health SciencesPublic and Occupational HealthRandomized Controlled Trials as TopicMultidisciplinaryVDP::Medisinske Fag: 700::Helsefag: 800::Samfunnsmedisin sosialmedisin: 801StatisticsQRHIV diagnosis and managementMetaanalysisResearch AssessmentVaccination and ImmunizationPeer reviewTreatment OutcomeAnti-Retroviral AgentsVDP::Medisinske Fag: 700::Helsefag: 800Medical MicrobiologyMeta-analysisViral PathogensVirusesPhysical SciencesMedicineFemalePathogensResearch Articlemedicine.medical_specialtyDrug Research and DevelopmentSystematic ReviewsScienceImmunologyMEDLINEAntiretroviral TherapyResearch and Analysis MethodsMicrobiologyPeer GroupQuality of life (healthcare)Antiviral TherapyRetrovirusesMental Health and PsychiatrymedicineHumansClinical TrialsStatistical MethodsMicrobial PathogensPharmacologybusiness.industryLentivirusOrganismsSocial SupportBiology and Life SciencesHIVOdds ratioRandomized Controlled TrialsDiagnostic medicineFamily medicineRelative riskMedical Risk FactorsHIV-1Preventive MedicineClinical MedicineVDP::Medical disciplines: 700::Health sciences: 800::Community medicine Social medicine: 801businessMathematics
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Low bone mineral density in HIV-positive young Italians and migrants.

2020

Background Human immunodeficiency virus (HIV) infected individuals may have osteoporosis. We aimed to evaluate the bone mineral density (BMD) in naïve antiretroviral (ARV) treated HIV positive patients comparing native Italian group (ItG) to a Migrants group (MiG) upon arrival in Italy. Methods We conducted a cross-sectional study on 83 HIV patients less than 50 years old. We used the dual-energy X-ray absorptiometry (DXA) within six months from the HIV diagnosis. Participants were categorized as having low BMD if the femoral neck or total lumbar spine Z-score was– 2 or less. Results MiG showed low BMD more often than ItG (37.5% vs.13.6%), especially for the female gender (16.7% vs. 0.0%). …

MaleRNA virusesEuropean PeopleCritical Care and Emergency MedicineBone densityEpidemiologyOsteoporosisHIV InfectionsLogistic regressionPathology and Laboratory Medicine0302 clinical medicineAbsorptiometry PhotonImmunodeficiency VirusesBone DensityMedicine and Health SciencesEthnicities030212 general & internal medicineVitamin DConnective Tissue DiseasesMusculoskeletal SystemTrauma MedicineBone mineralTransients and MigrantsMultidisciplinaryQRvirus diseasesHuman immunodeficiency virus (HIV)HIV diagnosis and managementosteoporosis.Middle AgedItalian Peoplemedicine.anatomical_structureAnti-Retroviral AgentsItalyMedical MicrobiologyConnective TissueBone FractureViral PathogensVirusesMedicineInfectious diseasesFemalePathogensAnatomyTraumatic InjuryResearch ArticleAdultMedical conditionsmedicine.medical_specialtyScience030209 endocrinology & metabolismViral diseasesMicrobiology03 medical and health sciencesLumbarSex FactorsRheumatologyInternal medicineRetrovirusesmedicineVitamin D and neurologyHumansBoneMicrobial PathogensSkeletonFemoral neckbusiness.industryLentivirusOrganismsBiology and Life SciencesHIVBone fracturemedicine.diseaseDiagnostic medicineCD4 Lymphocyte CountCross-Sectional StudiesLogistic ModelsBiological TissueMedical Risk FactorsPeople and PlacesOsteoporosisPopulation GroupingsbusinessPloS one
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Increased liver stiffness is associated with mortality in HIV/HCV coinfected subjects: The French nationwide ANRS CO13 HEPAVIH cohort study

2019

Background The association between liver stiffness measurements (LSM) and mortality has not been fully described. In particular the effect of LSM on all-cause mortality taking sustained virological response (SVR) into account needs further study. Methods HIV/HCV participants in the French nation-wide, prospective, multicenter ANRS CO13 HEPAVIH cohort, with ≥1 LSM by FibroScan (FS) and a detectable HCV RNA when the first valid FS was performed were included. Cox proportional hazards models with delayed entry were performed to determine factors associated with all-cause mortality. LSM and SVR were considered as time dependent covariates. Results 1,062 patients were included from 2005 to 2015 …

MaleRNA virusesSustained Virologic ResponseMetabolic disordersSocial SciencesHIV InfectionsHepacivirusmedicine.disease_causeGastroenterology0302 clinical medicineImmunodeficiency VirusesRisk FactorsPsychologyAlcohol consumptionProspective Studies030212 general & internal medicineProspective cohort studyPathology and laboratory medicineMultidisciplinaryDeath ratesCoinfectionHepatitis C virusMortality rateQHazard ratioRvirus diseasesHepatitis CMiddle AgedMedical microbiologyAddicts3. Good healthLiverDrug usersVirusesCohortElasticity Imaging TechniquesMedicineFemale030211 gastroenterology & hepatologyFrancePathogensResearch ArticleCohort studyAdultmedicine.medical_specialtyScienceHepatitis C virusLiver fibrosisAddictionGastroenterology and HepatologyAntiviral AgentsMicrobiology03 medical and health sciencesPopulation MetricsInternal medicineRetrovirusesmedicineHumansMortalityLiver diseasesProportional Hazards ModelsNutritionMedicine and health sciencesBiology and life sciencesFlavivirusesPopulation Biologybusiness.industryProportional hazards modelLentivirusOrganismsViral pathogensHIVHepatitis C Chronicmedicine.diseaseHepatitis virusesMicrobial pathogensDiet[SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologiebusinessPLOS ONE
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A population-based screening for hepatitis C antibodies and active infection using a point-of-care test in a low prevalence area

2020

Background Data on the true prevalence of hepatitis C virus (HCV) infection in the general population is essential. We evaluated a program implementing free universal HCV screening using a non-invasive point-of-care test (POCT) (OraQuick-HCV rapid test) in oral fluid in an urban area in Valencia, South-Eastern Spain. Methods A cross-sectional study was performed during 2015–2017. Free HCV screening was offered by regular mail to 11,500 individuals aged 18 and over, randomly selected from all census residents in the Health Department. All responding participants filled in a questionnaire about HCV infection risk factors and were tested in their tertiary Hospital. In those with a positive POC…

MaleRNA virusesSustained Virologic ResponseUrban PopulationEpidemiologyPhysiologyCross-sectional studyHepacivirusmedicine.disease_causeBiochemistryGeographical locations0302 clinical medicineRisk FactorsImmune PhysiologyPrevalence030212 general & internal medicineEnzyme-Linked ImmunoassaysYoung adultPathology and laboratory medicineAged 80 and overResponse rate (survey)education.field_of_studyImmune System ProteinsMultidisciplinaryHepatitis C virusQRMiddle AgedMedical microbiologyHepatitis CEuropeVirusesRNA ViralMedicineFemale030211 gastroenterology & hepatologyStandardized ratePathogensResearch ArticleAdultmedicine.medical_specialtyAdolescentPoint-of-Care SystemsScienceHepatitis C virusPoint-of-care testingImmunologyPopulationResearch and Analysis MethodsAntiviral AgentsMicrobiologyAntibodiesYoung Adult03 medical and health sciencesInternal medicinemedicineHumansEuropean UnionRisk factorImmunoassayseducationAgedMedicine and health sciencesBiology and life sciencesFlavivirusesbusiness.industryOrganismsViral pathogensProteinsHepatitis C AntibodiesFibrosisHepatitis virusesMicrobial pathogensHealth CareCross-Sectional StudiesSpainMedical Risk FactorsImmunologic TechniquesInterferonsPeople and placesbusinessDevelopmental BiologyPLOS ONE
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Management of suspected COVID-19 patients in a low prevalence region

2020

The spread of the SARS-CoV-2 infection among population has imposed a re-organization of healthcare services, aiming at stratifying patients and dedicating specific areas where patients with suspected COVID-related respiratory disease could receive the necessary health care assistance while waiting for the confirmation of the diagnosis of COVID-19 disease. In this scenario, the pathway defined as a “grey zone” is strongly advocated. We describe the application of rules and pathways in a regional context with low diffusion of the infection among the general population in the attempt to provide the best care to respiratory patients with suspected COVID-19. To date, this process has avoided t…

MaleRespiratory Tract DiseasesDiseaseClinical Laboratory Technique0302 clinical medicineCOVID-19 TestingHealth carePrevalence030212 general & internal medicinehealth-care managementeducation.field_of_studyrespiratory symptomsOrganizational InnovationGrey zoneCritical PathwayItalyCritical PathwaysFemaleCoronavirus InfectionsHumanPulmonary and Respiratory Medicinemedicine.medical_specialtyCoronavirus disease 2019 (COVID-19)Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)PopulationPneumonia ViralContext (language use)Settore MED/10 - Malattie Dell'Apparato RespiratorioDiagnosis Differential03 medical and health sciencesBetacoronavirusmedicineHumansIntensive care medicineeducationPandemicsAgedrespiratory symptomInfection ControlBetacoronavirubusiness.industryCoronavirus InfectionClinical Laboratory TechniquesSARS-CoV-2Hot TopicCOVID-19Patient Care ManagementCOVID-19 Drug Treatment030228 respiratory systemEpidemic outbreakbusinessChronic Respiratory Disease
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Where are patients missed in the tuberculosis diagnostic cascade? A prospective cohort study in Ghana.

2020

BackgroundGhana's national prevalence survey showed higher than expected tuberculosis (TB) prevalence, indicating that many people with TB are not identified and treated. This study aimed to identify gaps in the TB diagnostic cascade prior to starting treatment.MethodsA prospective cohort study was conducted in urban and rural health facilities in south-east Ghana. Consecutive patients routinely identified as needing a TB test were followed up for two months to find out if sputum was submitted and/or treatment started. The causal effect of health facility location on submitting sputum was assessed before risk factors were investigated using logistic regression.ResultsA total of 428 persons …

MaleRural PopulationBacterial DiseasesRNA virusesResearch FacilitiesUrban PopulationPhysiologyHIV InfectionsPathology and Laboratory MedicineGhanaCohort StudiesGeographical Locations0302 clinical medicineImmunodeficiency VirusesHealth facilityHealth careOdds RatioMedicine and Health SciencesCoughingProspective Studies030212 general & internal medicineProspective cohort studyMultidisciplinaryRural healthQRMiddle AgedBody FluidsInfectious DiseasesMedical MicrobiologyResearch DesignViral PathogensVirusesTuberculosis Diagnosis and ManagementMedicineFemaleAnatomyPathogensmedicine.symptomResearch LaboratoriesResearch ArticleCohort studyAdultmedicine.medical_specialtyTuberculosisAdolescentScience030231 tropical medicineResearch and Analysis MethodsMicrobiologyYoung Adult03 medical and health sciencesSigns and SymptomsTuberculosis diagnosisDiagnostic MedicineRetrovirusesmedicineHumansTuberculosisMicrobial PathogensAgedbusiness.industryLentivirusSputumOrganismsBiology and Life SciencesHIVTropical Diseasesmedicine.diseaseMucusFamily medicinePeople and PlacesAfricaSputumHealth FacilitiesPhysiological ProcessesbusinessGovernment LaboratoriesPLoS ONE
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Differences between Human Plasma and Serum Metabolite Profiles

2011

BackgroundHuman plasma and serum are widely used matrices in clinical and biological studies. However, different collecting procedures and the coagulation cascade influence concentrations of both proteins and metabolites in these matrices. The effects on metabolite concentration profiles have not been fully characterized.Methodology/principal findingsWe analyzed the concentrations of 163 metabolites in plasma and serum samples collected simultaneously from 377 fasting individuals. To ensure data quality, 41 metabolites with low measurement stability were excluded from further analysis. In addition, plasma and corresponding serum samples from 83 individuals were re-measured in the same plate…

MaleSerumClinical Research DesignEpidemiologyScienceMetaboliteProtein metabolismType 2 diabetesPharmacologyBiologyBiochemistryPlasmachemistry.chemical_compoundDiagnostic MedicineBlood plasmaPathologymedicineMetabolomeHumansClinical EpidemiologyBiologyAgedAged 80 and overClinical ChemistryReproducibilityMultidisciplinaryChromatographyQChromatography; Metabolomics; Collection; Samples; Issues; AcidRReproducibility of ResultsMiddle Agedmedicine.diseaseClinical Laboratory SciencesBiomarker EpidemiologychemistrySmall MoleculesBlood ChemistryMetabolomeMedicineBiomarker (medicine)FemaleBiomarkersDrug metabolismResearch ArticleGeneral PathologyPLoS ONE
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