Search results for "Ligo"
showing 10 items of 1427 documents
Migration of Leukocytes into Filters Coated Homogeneously with Immune Complexes, Antigens, Lectins or Tripeptides
1980
Cellulose nitrate filters were incubated in solutions of albumin, a chemotactically active tripeptide (f-Met-Leu-Phe), immune complexes or lectins and afterwards washed with buffer. They showed a dose-dependent increased leukocyte migration, when tested in typical Boyden chambers in comparison to filters treated only with buffer. The tripeptide, the immune complexes and the lectins were stimulatory at very low concentrations and acted inhibitory at high concentrations. Treating filters with formaldehyde or glutardialdehyde had no clear stimulatory effect. These findings extend earlier observations obtained with casein. They show that cells move very effectively on solid substrata in the abs…
Secretion and antigenicity of hepatitis B virus small envelope proteins lacking cysteines in the major antigenic region.
1995
Abstract Disulfide bonds are of crucial importance for the structure and antigenic properties of the hepatitis B virus (HBV) envelope. We have evaluated the role of the eight highly conserved cysteines of the major antigenic region for assembly, secretion, and antigenicity of the envelope proteins. Mutants carrying single or multiple substitutions of alanine for cysteine were analyzed using epitope tagging and transient expression in COS-7 cells. The only single cysteines found to be indispensable for efficient secretion were Cys-107 and Cys-138, but double mutation of Cys-137 and Cys-139 also created a block to secretion. Poorly secreted mutants formed aberrant oligomeric structures. The a…
Cytoprotective organoselenium compounds for oligodendrocytes
2021
Abstract Herein we report the synthesis of peptide-like and tetrazole-based organoselenium compounds via Ugi and Ugi-azide reactions, respectively. The organoselenium compounds' intrinsic cytoprotective and antioxidant capacities were evaluated in 158 N and 158JP murine oligodendrocytes. Furthermore, their redox properties were theoretically evaluated using Molecular Operating Environment-docking studies. Most of the compounds did not exhibit any cytotoxicity against the 158JP and 158 N cells. Among the tested compounds, the tetrazole- (e.g., 6, 7, and 9) and the pseudopeptide-based organoselenium compounds (e.g., 11, 15, and 17) displayed antioxidant properties. On the other hand, the quin…
Computational approach to design of aptamers to the receptor binding domain of SARS-CoV-2
2021
The aim of the research. In this work, in silico selection of DNA-aptamers to the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein was performed using molecular modeling methods. Material and methods. A new computational approach to aptamer in silico selection is based on a cycle of simulations, including the stages of molecular modeling, molecular docking, molecular dynamic simulations, and quantum chemical calculations. To verify the obtained calculated results flow cytometry, fluorescence polarization, and small-angle X-ray scattering methods were applied. Results. An initial library consisted of 256 16-mer oligonucleotides was modeled. Based on molecular docking results, th…
Study of oligogalacturonides-triggered Nitric Oxide (NO) production provokes new questioning about the origin of NO biosynthesis in plants
2014
Addendum to: Rasul S, Dubreuil-Maurizi C, Lamotte O, Koen E, Poinssot B, Alcaraz G, et al. Nitric oxide production mediates oligogalacturonide-triggered immunity and resistance to Botrytis cinerea in Arabidopsis thaliana. Plant Cell Environ 2012; PMID:22394204; http://dx.doi. org/10.1111/j.1365-3040.2012.02505.x.; International audience; We investigated the production and function of nitric oxide (NO) in Arabidopsis thaliana leaf discs as well as whole plants elicited by oligogalacturonides (OGs). Using genetic, biochemical and pharmacological approaches, we provided evidence that OGs induced a Nitrate Reductase (NR)-dependent NO production together with an increased NR activity and NR tran…
Comparison of global responses to mild deficiency and excess copper levels in Arabidopsis seedlings
2013
[EN] Copper is an essential micronutrient in higher plants, but it is toxic in excess. The fine adjustments required to fit copper nutritional demands for optimal growth are illustrated by the diverse, severe symptoms resulting from copper deficiency and excess. Here, a differential transcriptomic analysis was done between Arabidopsis thaliana plants suffering from mild copper deficiency and those with a slight copper excess. The effects on the genes encoding cuproproteins or copper homeostasis factors were included in a CuAt database, which was organised to collect additional information and connections to other databases. The categories overrepresented under copper deficiency and copper e…
CAUSE DI OLIGOANALGESIA IN AREA D’EMERGENZA AL TEMPO DELLE GRANDI IMMIGRAZIONI
2013
Obiettivo. L’AOU Policlinico di Palermo ha un centro dedi- cato ai bisogni sanitari degli immigrati regolari e clandestini, pertanto l’area di emergenza (PS) è il punto di riferimento di questa popolazione straniera. Il dolore, causa principale di accesso al PS, è sottotrattato. Le ipotesi delle differenze nella qualità del sollievo sono svariate, e comprendono anche etnia, razza, sesso ed età. Anche il modo in cui pazienti, di culture differenti, esprimono il proprio dolore e le interazioni tra medici e pazienti di diversa origine etnica possono influenza- re la valutazione. Abbiamo cercato di determinare se il sollie- vo dal dolore in PS è associato negativamente con la percezio- ne del m…
Oxidative stress response of tumor cells: microarray-based comparison between artemisinins and anthracyclines
2004
The antimalarial artemisinins also reveal profound cytotoxic activity against tumor cells. Artemisinins harbor an endoperoxide bridge whose cleavage results in the generation of reactive oxygen species (ROS) and/or artemisinin carbon-centered free radicals. Established cancer drugs such as anthracyclines also form ROS and free radicals that are responsible for the cardiotoxicity of anthracyclines. In contrast, artemisinins do not reveal cardiotoxicity. In the present investigation, we compared the cytotoxic activities of different artemisinins (artemisinin, artesunate, arteether, artemether, artemisitene, dihydroartemisinylester stereoisomers) in 60 cell lines of the National Cancer Institu…
GWTC-1: A Gravitational-Wave Transient Catalog of Compact Binary Mergers Observed by LIGO and Virgo during the First and Second Observing Runs
2019
We present the results from three gravitational-wave searches for coalescing compact binaries with component masses above 1$\mathrm{M}_\odot$ during the first and second observing runs of the Advanced gravitational-wave detector network. During the first observing run (O1), from September $12^\mathrm{th}$, 2015 to January $19^\mathrm{th}$, 2016, gravitational waves from three binary black hole mergers were detected. The second observing run (O2), which ran from November $30^\mathrm{th}$, 2016 to August $25^\mathrm{th}$, 2017, saw the first detection of gravitational waves from a binary neutron star inspiral, in addition to the observation of gravitational waves from a total of seven binary …
A first search for coincident gravitational waves and high energy neutrinos using LIGO, Virgo and ANTARES data from 2007
2013
A search for high-energy neutrinos coming from the direction of the Sun has been performed using the data recorded by the ANTARES neutrino telescope during 2007 and 2008. The neutrino selection criteria have been chosen to maximize the selection of possible signals produced by the self-annihilation of weakly interacting massive particles accumulated in the centre of the Sun with respect to the atmospheric background. After data unblinding, the number of neutrinos observed towards the Sun was found to be compatible with background expectations. The 90% CL upper limits in terms of spin-dependent and spin-independent WIMP-proton cross-sections are derived and compared to predictions of two sup…