Search results for "Linea"
showing 10 items of 7724 documents
Leukemia-associated activating mutation of Flt3 expands dendritic cells and alters T cell responses
2016
Lau et al. show that the FLT3-ITD mutation directly affects dendritic cell development in preleukemic mice, indirectly modulating T cell homeostasis and supporting the expansion of regulatory T cells.
Structural Basis of the High Affinity Interaction between the Alphavirus Nonstructural Protein-3 (nsP3) and the SH3 Domain of Amphiphysin-2
2016
We show that a peptide from Chikungunya virus nsP3 protein spanning residues 1728–1744 binds the amphiphysin-2 (BIN1) Src homology-3 (SH3) domain with an unusually high affinity (Kd 24 nM). Our NMR solution complex structure together with isothermal titration calorimetry data on several related viral and cellular peptide ligands reveal that this exceptional affinity originates from interactions between multiple basic residues in the target peptide and the extensive negatively charged binding surface of amphiphysin-2 SH3. Remarkably, these arginines show no fixed conformation in the complex structure, indicating that a transient or fluctuating polyelectrostatic interaction accounts for this …
Development of a method to determine axitinib, lapatinib and afatinib in plasma by micellar liquid chromatography and validation by the European Medi…
2017
A method based on micellar liquid chromatography to quantify the tyrosine kinase inhibitors axitinib, lapatinib and afatinib in plasma is reported. The sample pretreatment was a simple 1/5-dilution in a pure micellar solution, filtration and direct injection, without requiring extraction or purification steps. The three drugs were resolved from the matrix in 17 min, using an aqueous solution of 0.07 M sodium dodecyl sulfate – 6.0% 1-pentanol, buffered at pH 7 with 0.01 M phosphate salt as mobile phase, running under isocratic mode at 1 mL/min through a C18 column. The detection was performed by absorbance at 260 nm. An accurate mathematical relationship was established between the retention…
Mass, phylogeny, and temperature are sufficient to explain differences in metabolic scaling across mammalian orders?
2016
Abstract Whether basal metabolic rate‐body mass scaling relationships have a single exponent is highly discussed, and also the correct statistical model to establish relationships. Here, we aimed (1) to identify statistically best scaling models for 17 mammalian orders, Marsupialia, Eutheria and all mammals, and (2) thereby to prove whether correcting for differences in species’ body temperature and their shared evolutionary history improves models and their biological interpretability. We used the large dataset from Sieg et al. (The American Naturalist 174, 2009, 720) providing species’ body mass (BM), basal metabolic rate (BMR) and body temperature (T). We applied different statistical ap…
The effect of visual motion stimulus characteristics on vection and visually induced motion sickness
2019
Abstract Several factors contribute to the likelihood of experiencing illusory sensations of self-motion (i.e., vection) in Virtual Reality (VR) applications. VR users can also experience adverse effects such as disorientation, oculomotor issues, or nausea known as visually induced motion sickness (VIMS). The goal of the present study was to systematically investigate three characteristics of visual motion stimuli—speed, density, and axis of rotation—and how they relate to both vection and VIMS. Two experiments were conducted. In Experiment 1, a stereoscopic stimulus containing a star field of white spheres on a black background was presented to 21 participants. The stimulus contained linea…
Ultrasonographic Measurements of the Achilles Tendon in Elderly Athletes and Sedentary Men
1994
Ultrasonography was performed on the Achilles tendon of the dominant limb of 18 male elderly athletes and 11 sedentary men, aged 70 to 80 years. The tendons were examined with a real-time linear array scanner using a 7.5 MHz transducer. Both longitudinal and transverse images were taken. The mean width of the Achilles tendon was significantly larger in the athletes than in the control subjects. The tendon thickness and cross-sectional area did not differ significantly between the groups, but the figures may indicate a tendency for larger cross-sectional area in the athletes. The results suggest tendon hypertrophy following long-term training.
Stage-specific control of oligodendrocyte survival and morphogenesis by TDP-43
2021
AbstractGeneration of oligodendrocytes in the adult brain enables both adaptive changes in neural circuits and regeneration of myelin sheaths destroyed by injury, disease, and normal aging. This transformation of oligodendrocyte precursor cells (OPCs) into myelinating oligodendrocytes requires processing of distinct mRNAs at different stages of cell maturation. Although mislocalization and aggregation of the RNA binding protein TDP-43 occur in both neurons and glia in neurodegenerative diseases, the consequences of TDP-43 loss within different stages of the oligodendrocyte lineage are not well understood. By performing stage-specific genetic inactivation of Tardbp in vivo, we show that olig…
Multisite field potential recordings and analysis of the impulse propagation pattern in cardiac cells culture
2007
To provide further insights into the impulse propagation between cardiac myocytes, we performed multiparametric studies of excitation spread with cellular resolution in confluent monolayers of cultured cardiomyocytes (CM). Simultaneous paired intracellular recordings of action potentials in two individual CM revealed slight periodic spontaneous advances/delays in the interspike time lag. Multisite field potential recordings performed with microelectrode arrays (MEA) confirmed random and iterative cycle-to-cycle changes in the direction of excitation spread. These local spontaneous variations in the cardiac impulse propagation pathways may be a safety process protecting against microscopical…
SORT-CC: A procedure for the statistical treatment of free sorting data
2008
International audience; A statistical approach for the analysis of free sorting data is discussed. In a first stage, the sorting data from each subject are arranged into a dataset consisting of indicator variables which reflect the memberships of the stimuli to the groups formed by the subject under consideration. Thereafter, an appropriate standardization is applied on these data and a three way statistical method, namely Common Components and Specific Weights Analysis, is performed on the datasets thus obtained. This makes it possible to take account of the individual differences among the subjects and to depict graphical displays showing the relationships among the stimuli on the one han…
Betweenness Centrality for Networks with Non-Overlapping Community Structure
2018
Evaluating the centrality of nodes in complex networks is one of the major research topics being explored due to its wide range of applications. Among the various measures that have been developed over the years, Betweenness centrality is one of the most popular. Indeed, it has proved to be efficient in many real-world situations. In this paper, we propose an extension of the Betweenness centrality designed for networks with nonoverlapping community structure. It is a linear combination of the so-called “local” and “global” Betweenness measures. The Local measure takes into account the influence of a node at the community level while the global measure depends only on the interactions betwe…