Search results for "Lost"

Application of HPP in food fermentation processes

2020

Abstract High pressure processing (HPP) is widely used in the food industry for nonthermal pasteurization of juices, ready-to-eat meals, dairy products, pet food, etc. The pasteurization effect is induced by damaging the membranes of microorganisms (leading to cell lysis) as well as by protein denaturation, thus interrupting cellular functions such as nutrient uptake, DNA replication, etc. Nevertheless, as a thermodynamic variable, pressure can also be used to enhance the fermentative processes if applied at sublethal levels (up to 50/60 MPa) to induce metabolic shifts in microorganisms, This allows accelerating the fermentative processes or even obtaining different compounds resulting from…

Gastrin-cholecystokinin-like immunoreactivity in the central nervous system of the milkweed bug, Spilostethus pandurus. Ultrastructural aspects of th…

1995

Summary All the ganglia belonging to the central nervous system of adults of the milkweed bug Spilostethus pandurus (Hemiptera) were screened immunohistochemically for vertebrate gastrin-cholecystokinin (CCK-8(s))-like peptides. Several large reactive perikarya are present in the median part of the protocerebrum, their processes extending to the dorsal ‘aorta’. These cell bodies are also paraldehyde fuchsin-positive, ie they are A-type cells. In the lateral part of the protocerebrum, in the deutocerebrum and tritocerebrum, and in the suboesophageal, prothoracic and abdominal ganglia, a few immunoreactive cell bodies send axonal processes into their respective neuropiles. The A-type cells re…

Residential greenness-related DNA methylation changes

2021

Abstract Background Residential greenness has been associated with health benefits, but its biological mechanism is largely unknown. Investigation of greenness-related DNA methylation profiles can contribute to mechanistic understanding of the health benefits of residential greenness. Objective To identify DNA methylation profiles associated with greenness in the immediate surroundings of the residence. Methods We analyzed genome-wide DNA methylation in 1938 blood samples (982 participants) from the Swiss Cohort Study on Air Pollution and Lung and Heart Diseases in Adults (SAPALDIA). We estimated residential greenness based on normalized difference vegetation index at 30 × 30 m cell (green3…

APPRENDERE AL MUSEO STUDIO DI CASO - LA VALIDITA’ DI UN MODELLO DI APPROCCIO DI TIPO ESPLORATIVO-ESPERENZIALE AL BENE MUSEALE COME OGGETTO DALLE MOLT…

Il museo, luogo privilegiato per l'educazione al Patrimonio culturale, per la sua funzione educativa, può collaborare produttivamente con l'istituzione scolastica, in vista di un apprendimento efficace basato sulla valorizzazione dei suoi oggetti e collezioni. Lo studio di caso oggetto del presente lavoro, intende mostrare con approccio qualitativo, la validità di un modello teorico-applicativo per la formazione dei docenti, basato sull'esplorazione della concezione di museo, sulla considerazione del valore antropologico del conservare, sulle potenzialità apprenditive delle "cose" che possono fornire innumerevoli spunti per una programmazione multidisciplinare ed interdisciplinare.

Exploring the mechanisms by which reindeer droppings induce fen peat methane production

2021

Abstract Peatlands, especially fens, are known to emit methane. Reindeer (Rangifer tarandus) use mires mainly as spring and summer pastures. In this work we observed that adding reindeer droppings to fen peat increased the potential methane production by 40%. This became apparent when droppings originating from reindeer kept in pen or pasture in winter were added to methanogenic fen peat samples. The droppings introduced Methanobacteriaceae (Methanobrevibacter; > 90% of the mcrA MiSeq reads) to the peat, which was originally populated by Methanosarcinaceae, Methanosaetaceae, Methanoregulaceae, Methanobacteriaceae, Methanomassiliicoccaceae, Methanocellaceae and Methanomicrobiaceae. The origi…

Selenium contents of human milk and infant formulas in Spain.

1999

The selenium content of Spanish human milk samples and different milk-based and soy-based infant formulas has been estimated by using a flow injection hydride atomic absorption spectrometric method after microwave digestion of the organic matter. Mean values of 11.4 +/- 3.7 and 10.7 +/- 4.6 ng/ml for colostrum and transitional milk, 8.4 +/- 3.4 and 5.3 +/- 1.9 ng/ml for mature milk at 1 month and up to 2 months respectively, was obtained. These values are close to those reported by others authors in Europe, and lower than the ones from the US, Japan and Korea. Selenium contents of the analyzed infants' formulas ranged from 2.7 to 9.6 ng/ml and from 1.8 to 7.5 ng/ml for soy and milk-based in…

Use of delamanid for M/XDR-TB treatment under programmatic conditions in Latvia

2016

Introduction. Since 2014 Delamanid (Dlm) was introduced in M/XDR-TB treatment under programmatic conditions. The aim of this study was to analyse experience of Dlm use. Methods. For all MDR-TB cases treatment regimens (Tx) are designed by MDR-TB expert committee. Dlm is prescribed for patients with risk of failure of Tx due to resistance or severe intolerance of key 2 nd line drugs. We analysed clinical and demographic data for all patients who received Dlm during the period of July 2014 to February 2016. Data were collected from patient9s medical records. Results. In total 20 MDR TB patients received Dlm: 3 female and 17 male with median age 46 years, including 2 children (11 and 13 years …

The main determinant of furosemide inhibition on GABA(A) receptors is located close to the first transmembrane domain.

1998

Inhibitory GABA(A) receptors are regulated by numerous allosteric modulators, the most receptor-subtype specific of which is furosemide. It recognises receptors of the subunit composition alpha6beta2/3gamma2, restricted to cerebellar granule cells. To locate furosemide's site of action we constructed chimeras of the furosemide-sensitive alpha6 and the furosemide-insensitive alpha1 subunit, and expressed and studied them together with the beta3 and gamma2 subunits in Xenopus oocytes by the two-electrode voltage clamp technique. The inhibition of GABA-induced currents by furosemide mainly depended on a short domain proximal to the first transmembrane region of the alpha6 subunit.

Evaluation of the IKKβ Binding of Indicaxanthin by Induced-Fit Docking, Binding Pose Metadynamics, and Molecular Dynamics

2021

Background: Indicaxanthin, a betaxanthin belonging to the betalain class of compounds, has been recently demonstrated to exert significant antiproliferative effects inducing apoptosis of human melanoma cells through the inhibition of NF-κB as the predominant pathway. Specifically, Indicaxanthin inhibited IκBα degradation in A375 cells. In resting cells, NF-κB is arrested in the cytoplasm by binding to its inhibitor protein IκBα. Upon stimulation, IκBα is phosphorylated by the IKK complex, and degraded by the proteasome, liberating free NF-κB into the nucleus to initiate target gene transcription. Inhibition of the IKK complex leads to the arrest of the NF-κB pathway.Methods: To acquire deta…

Single-digit nanomolar inhibitors lock the aromatase active site via a dualsteric targeting strategy

2022

The most frequently diagnosed breast cancer (BC) type in women expresses estrogen receptor (ER) , depends on estrogens for its growth, being classified as ER positive (ER+). The gold standard therapy for the treatment of this tumor relies on the inhibition of the aromatase enzyme, which catalyzes estrogen biosynthesis. Despite the clinical success of current aromatase inhibitors (AIs), after prolonged therapeutic regimens, BC ER + patients experience acquired resistance and disease relapse. This points up the urgent need for a newer generation of AIs able to overcome resistance issues, while mitigating toxicity and side effects of current therapies. Here we performed the synthesis, biologic…