Search results for "Lps"

LPS challenge regulates gene expression and tissue localization of a Ciona intestinalis gene through an alternative polyadenylation mechanism

2013

Characterization of a novel LPS-induced peptide from Ciona intestinalis with immune modulatory activities

2015

Porphyromonas gingivalis, Periodontal and Systemic Implications: A Systematic Review

2019

In recent scientific literature, oral infections and systemic manifestations, or correlations between oral health and systemic diseases are a topic of discussion. Porphyromonas gingivalis is one of the bacteria implicated in the biofilm formation of bacterial plaque, and plays an important role in the progression of periodontal disease. In this systematic review authors have evaluated the literature of the last 10 years on P. gingivalis and all the systemic implications proven. This study therefore evaluates all the districts of the organism in which this bacterium may have implications. From the results it emerges that P. gingivalis has implications in the onset of different systemic patho…

Transcriptomic and Bioinformatic Analyses Identifying a Central Mif-Cop9-Nf-kB Signaling Network in Innate Immunity Response of Ciona robusta

2023

The Ascidian C. robusta is a powerful model for studying innate immunity. LPS induction activates inflammatory-like reactions in the pharynx and the expression of several innate immune genes in granulocyte hemocytes such as cytokines, for instance, macrophage migration inhibitory factors (CrMifs). This leads to intracellular signaling involving the Nf-kB signaling cascade that triggers downstream pro-inflammatory gene expression. In mammals, the COP9 (Constitutive photomorphogenesis 9) signalosome (CSN) complex also results in the activation of the NF-kB pathway. It is a highly conserved complex in vertebrates, mainly engaged in proteasome degradation which is essential for maintaining proc…

Attività immunomodulatoria del peptide PAR37 derivato dall'allergene maggiore di Parietaria judaica ParJ1.0101

2013

Fattore anti-lps da Parietaria Judaica

2009

L’endotossina è una tossina microbica, parte integrante della membrana esterna della parete di batteri Gram-negativi che viene rilasciata completamente con la lisi del batterio. È costituita da sub-unita’ molecolari che hanno dimensioni comprese tra 10.000 e 20.000 Daltons, mentre le aggregazioni hanno dimensioni di circa 100.000 Daltons. Un esempio tipico di endotossina è rappresentato dal lipopolisaccaride (LPS) che è presente nella membrana esterna dei batteri Gram-negativi. Le endotossine sono le principali responsabili delle conseguenze cliniche delle infezioni da batteri gram-negativi. Infatti, l’endotossina è ritenuta responsabile della patogenesi della sepsi, dello shock settico e d…

β - glikānu pretiekaisuma efektu pārbaude in vitro

2020

β – glikāni ir dabiski polisaharīdi un ir atrodami baktēriju un sēnīšu šūnu sieniņās. Tie ir sastopami dažādos avotos, kā, piemērām, sēnēs, baktērijās un graudaugos. Tieši dažādo avotu strukturālās atšķirības ir saistītas ar dažādām farmakoloģiskām īpašībām un bioloģiskām aktivitātēm. Visi β – glikāni ir glikozes polimēri, kas savienoti ar 1 → 3 lineāru β – glikozīdisku ķēdi, taču sānu ķēdes saites katram β – glikānam ir atsķirīgas un specifiskas. Uzskata, ka uzturā bieži lietotajiem un labi zināmiem β – glikāniem, kas ir iegūti no rauga un auzām, piemīt imūnsistēmu stiprinoša darbība un tie aktivizē imūnsistēmas šūnas organismā. Darba mērķis bija noskaidrot vai dabīgajiem rauga un auzu β –…

Phenotype characterization of the anti-inflammatory behavior of decidual endothelial cells

2009

COX-2 ekspresijas inducēšana LPS iekaisuma modelī neiroblastomas SH-SY5Y šūnu līnijā

2015

COX-2 ir COX izoenzīms, kas atbild par arahidonskābes metabolismu, sintezējot bioloģiski aktīvus iekaisuma mediatorus – prostanoīdus. Perifērijā COX-2 ir inducējama izoforma, bet smadzenēs – konstitutīva. Šī darba mērķis bija izpētīt LPS spēju inducēt COX-2 ekspresiju neiroblastomas SH-SY5Y šūnās. COX-2 ekspresijas noteikšanai tika izmantota imūnfluorescences metode. Rezultāti uzrādīja SH-SY5Y šūnu morfoloģijas izmaiņas dažādu LPS koncentrāciju (5 – 800 ng/ml) ietekmē. Pētījumā novēroja COX-2 ekspresiju kontroles šūnās normālos apstākļos, LPS koncentrācijā 5 ng/ml COX-2 ekspresijas indukciju, bet koncentrācijā 25 ng/ml un 50 ng/ml – supresiju. Statistiski ticamu rezultātu uzrādīja LPS konce…

Phenoloxidases of different sizes are modulated by LPS inoculation into ciona intestinalis tunic and pharynx

2015

In the present study, to further characterize the pro-phenoloxidase (proPO) and active phenoloxidase (PO) involved in the Ciona intestinalis inflammatory response, tunic and pharynx homogenate supernatants were separated on high pressure liquid chromatography and fractions were assayed for the PO activity before and after LPS inoculation, as well as before and after trypsin treatment which activates proPO. The LPS inoculation per se did not significantly change the basal PO activity of the tunic homogenate supernatant (THS) and pharynx homogenate supernatant (PHS) restricted in two confluent peaks, whereas a significant enhancement was observable after the trypsin treatment. This trypsin ef…