Search results for "M2"

Paclitaxel and beta-lapachone synergistically induce apoptosis in human retinoblastoma Y79 cells by downregulating the levels of phospho-Akt.

2009

Paclitaxel (PTX) and beta-lapachone (LPC) are naturally occurring compounds that have shown a large spectrum of anticancer activity. In this article we show for the first time that PTX/LPC combination induces potent synergistic apoptotic effects in human retinoblastoma Y79 cells. Combination of suboptimal doses of PTX (0.3 nM) and LPC (1.5 microM) caused biochemical and morphological signs of apoptosis at 48 h of treatment. These effects were accompanied by potent lowering in inhibitor of apoptosis proteins and by activation of Bid and caspases 3 and 6 with lamin B and PARP breakdown. PTX/LPC combination acted by favoring p53 stabilization through a lowering in p-Akt levels and in ps166-MDM…

Trace elemental composition of PM2.5 atmospheric aereosol in Palermo urban area

2009

Insulin-like growth factor 1 differentially regulates estrogen receptor-dependent transcription at estrogen response element and AP-1 sites in breast…

2007

Cross-talk between insulin-like growth factor 1 (IGF-1) and estrogen receptor alpha (ER) regulates gene expression in breast cancer cells, but the underlying mechanisms remain unclear. Here, we studied how 17-beta-estradiol (E2) and IGF-1 affect ER transcriptional machinery in MCF-7 cells. E2 treatment stimulated ER loading on the estrogen response element (ERE) in the pS2 promoter and on the AP-1 motif in the cyclin D1 promoter. On ERE, similar amounts of liganded ER were found at 1-24-h time points, whereas on AP-1, ER binding fluctuated over time. At 1 h, liganded ER was recruited to ERE together with histone acetyltransferases SRC-1 and p300, ubiquitin ligase E6-AP, histone methyltransf…

Analysis of p53 and mdm2 proteins in malignant fibrous histiocytoma in absence of gene alteration: prognostic significance.

2000

TP53 and MDM2 genes and their protein expression were evaluated in frozen and paraffin-embedded tissue from 27 patients with malignant fibrous histiocytoma to elucidate the relationship between them, their implication in tumor progression mechanisms and their possible diagnostic-prognostic value in malignant fibrous histiocytoma. Single-strand conformation polymorphism analysis and direct sequencing of polymerase chain reaction-amplified DNA were used to establish two TP53 mutations (7.4%): a point mutation and a 63-bp duplication. Amplification of the MDM2 gene was observed in two tumors (7.4%) by means of Southern-blot analysis, one of them also carrying the TP53 point mutation. Immunohis…

Analysis of the p53 and MDM-2 gene in acute myeloid leukemia

1996

The MDM-2 (murine double minute 2) gene codes for a cellular protein that can bind to the p53 tumor suppressor gene product, thereby functioning as a negative regulator of p53. In order to define the role of the MDM-2 gene in the pathogenesis of human acute myeloid leukemia, the expression and the sequence of the MDM-2 gene were examined in samples of bone marrow and/or peripheral mononuclear cells of 38 patients by using immunostaining, polymerase chain reaction (PCR), single strand conformation polymorphism, and sequencing. Immunohistochemical staining detected a weak accumulation of the MDM-2 protein in AML patients of FAB classification M4 and M5. RT-PCR analysis revealed a heterogeneou…

Stress darbavietā un tā mazināšanas iespējas SIA CENTRUM EUROPA.

2019

Stress darba vietā ir aktuāla problēma, ko pierāda veikto pētījumu rezultāti, ne tikai Latvijā, bet visā pasaulē. Stresa pārvaldība darba vietā var ievērojami samazināt izmaksas, kas ir saistītas ar psihoemocionālajiem darba vides riska faktoriem. Bakalaura darbā tiek aprakstītas populārākās teorijas un aktuālākā informācijas, kas ir saistīta ar stresu, tā cēloņiem un sekām darba vietā un ārpus tās. Teorētiskās literatūras analīzes laikā tika analizētas populārākās metodes stresa līmeņa samazināšanai darba vietā. Bakalaura darba pētījumā tika pielietota metode: informatīvo materiālu par stresu un tā pārvarēšanas metodēm ieviešana. Veikti divi mērījumu, kuru rezultāts ir darbinieku stresa lī…

Governance of global value chains after the Covid-19 pandemic: A new wave of regionalization?

2021

The disruption of the trade and investment activities of multinational enterprises as a consequence of the Covid-19 pandemic has reinvigorated the debate on the configuration of global value chains (GVCs) as well as the risks and challenges associated with offshoring. This article depicts how the pandemic might affect GVC configuration by driving a trend toward a more regional footprint in industries in which resilience and reliability are critical. Such a shift would create new opportunities for reshoring, and affect both the types of upgrading trajectories and the governance systems in value chains. The article also draws from the intersection of the global-strategy and value-chain field…

Changes in atmospheric pollutants during COVID-19

2022

Bakalaura darba “Atmosfēras piesārņojuma izmaiņas COVID-19 laikā” mērķis –Identificēt un izpētīt ar COVID-19 pandēmiju saistītās izmaiņas, konkrēti, pārvietošanās ierobežojumu un dzīves veida izmaiņu, ietekmi attiecībā uz atmosfēras piesārņojuma koncentrāciju rādītājiem Latvijas Republikas galvaspilsētā Rīgā. Pētījumā analizēti dati no Latvijas Ģeoloģijas un Meteoroloģijas (LVĢMC) centra novērojumu stacijām. COVID-19 pandēmijas ietekme bija jūtama visā pasaulē, tostarp arī Eiropā, jo ārkārtas stāvokļa un pārvietošanās ierobežojumu rezultātā bija vērojamas izmaiņas emisiju dinamikā atmosfērā. Sakarā ar izmaiņām darba norisē un darbinieku pāreju uz attālināto darbu, kā arī mācību un studiju p…

Cieto daļiņu atmosfēras piesārņojuma īstermiņa prognostiskā modeļa izstrāde Rīgas aglomerācijai

2016

Maģistra darba ietvaros izstrādāts cieto daļiņu atmosfēras piesārņojuma īstermiņa prognostiskais modelis diennakts periodam Rīgas aglomerācijai. Rīga ir viena no pilsētām, kurā ilgtermiņā novērojami cieto daļiņu normatīva pārsniegumi, tikai Dānijā, Igaunijā, Somijā, Īrijā, Luksemburgā un Apvienotā Karalistē pēdējos gados normatīvs nav ticis pārsniegts (EEA, 2015) Eiropā. Atmosfēras piesārņojuma līmeņa regulēšanā un uzlabošanā iespējams izmantot dažāda līmeņa pasākumus: drastiskus, regulējošus un informatīvus. Informatīvo pasākumu kopums ietver ne tikai informācijas sniegšanu par piesārņojuma līmeni, bet prognostisko modeļu izstrādi un iedzīvotāju brīdināšanu par sagaidāmo piesārņojuma līmen…

First Dark Matter Search Results from the XENON1T Experiment

2017

We report the first dark matter search results from XENON1T, a ∼2000-kg-target-mass dual-phase (liquid-gas) xenon time projection chamber in operation at the Laboratori Nazionali del Gran Sasso in Italy and the first ton-scale detector of this kind. The blinded search used 34.2 live days of data acquired between November 2016 and January 2017. Inside the (1042±12)-kg fiducial mass and in the [5,40] keVnr energy range of interest for weakly interacting massive particle (WIMP) dark matter searches, the electronic recoil background was (1.93±0.25)×10-4 events/(kg×day×keVee), the lowest ever achieved in such a dark matter detector. A profile likelihood analysis shows that the data are consisten…